This is a review about ROCK2, based on 10 published articles (read how Labome selects the articles), using ROCK2 antibody in all methods. It is aimed to help Labome visitors find the most suited ROCK2 antibody.
ROCK2 synonym: ROCK-II; Rho-associated, coiled-coil containing protein kinase 2; p164 ROCK-2; rho-associated, coiled-coil-containing protein kinase II

Knockout validation
Santa Cruz Biotechnologyrabbit polyclonal (H-85)
  • WB (knockout); mouse; fig 7a
In order to use ROCK1 and ROCK2 knock out mice to study doxorubicin-induced stress fiber disassembly and cell detachment, Santa Cruz Biotechnology ROCK2 antibody (Santa, sc-5561) was used in western blot (knockout) on mouse samples (fig 7a). Cell Cycle (2013) ncbi
Santa Cruz Biotechnology
goat polyclonal (C-20)
  • WB; human
In order to determine if the neurotrophic growth factor signaling pathway was altered in the placenta of selective serotonin reuptake inhibitors-treated, depressed, and healthy mothers, Santa Cruz Biotechnology ROCK2 antibody (SantaCruz, sc-1851) was used in western blot on human samples. PLoS ONE (2015) ncbi
rabbit polyclonal (H-85)
  • WB; human; 1:50-500
Santa Cruz Biotechnology ROCK2 antibody (Santa Cruz Biotechnology, sc5561) was used in western blot on human samples at 1:50-500. Reprod Biol Endocrinol (2013) ncbi
rabbit polyclonal (H-85)
  • WB (knockout); mouse; fig 7a
In order to use ROCK1 and ROCK2 knock out mice to study doxorubicin-induced stress fiber disassembly and cell detachment, Santa Cruz Biotechnology ROCK2 antibody (Santa, sc-5561) was used in western blot (knockout) on mouse samples (fig 7a). Cell Cycle (2013) ncbi
rabbit polyclonal (H-85)
  • WB; mouse
In order to , Santa Cruz Biotechnology ROCK2 antibody (Santa Cruz Biotechnology, sc-5561) was used in western blot on mouse samples. Cell Death Dis (2013) ncbi
not indicated
  • WB; human
Santa Cruz Biotechnology rabbit polyclonal anti-ROCKII antibody (H-85) was used in western blot to study the role for RhoE in keratinocyte differentiation and stratificationMol Biol Cell (2009) ncbi
not indicated
  • WB; human
Santa Cruz Biotechnology anti-ROCK-2 antibody was used in western blot to study the novel mechanism of G protein-dependent VASP phosphorylation.J Biol Chem (2005) ncbi
GeneTex
rabbit polyclonal
  • WB; human
In order to report that inhibition of eIF5A reduces pancreatic ductal adenocarcinoma cell migration, invasion, and metastasis in vitro and in vivo, GeneTex ROCK2 antibody (GeneTex, GTX108247) was used in western blot on human samples. J Biol Chem (2015) ncbi
Invitrogen
rabbit polyclonal
  • WB; human
In order to determine if the neurotrophic growth factor signaling pathway was altered in the placenta of selective serotonin reuptake inhibitors-treated, depressed, and healthy mothers, Invitrogen ROCK2 antibody (Thermo Scientific, PA5-34895) was used in western blot on human samples. PLoS ONE (2015) ncbi
Cell Signaling Technology
rabbit monoclonal (D1B1)
  • WB; human
In order to evaluate the effect of tumor necrosis factor on claudin-5 endothelial tight junction barriers, Cell Signaling Technology ROCK2 antibody (Cell Signaling, 9029) was used in western blot on human samples. PLoS ONE (2015) ncbi
BD Biosciences
not indicated
  • WB; human
BD Transduction Laboratories anit-ROCK2 antibody was used to perform western blot to study the different roles of ROCK1 and ROCK2 in regulating keratinocyte adhesion and terminal differentiation.PLoS ONE (2009) ncbi
not indicated
  • WB; human
BD Biosciences Pharmingen mouse monoclonal anti-ROCK II/ ROKa antibody was used in western blot to study inhibition of RhoA signaling required for both vaccinia morphogenesis and virus-induced cell motility.Science (2006) ncbi
Articles Reviewed
  1. Ken Fujimura et al. (2015). "Eukaryotic Translation Initiation Factor 5A (EIF5A) Regulates Pancreatic Cancer Metastasis by Modulating RhoA and Rho-associated Kinase (ROCK) Protein Expression Levels".PMID 26483550
  2. Paul R Clark et al. (2015). "Tumor necrosis factor disrupts claudin-5 endothelial tight junction barriers in two distinct NF-κB-dependent phases".PMID 25816133
  3. Helena Kaihola et al. (2015). "The effect of antenatal depression and selective serotonin reuptake inhibitor treatment on nerve growth factor signaling in human placenta".PMID 25611484
  4. Margaret O'Brien et al. (2013). "Decreased myometrial p160 ROCK-1 expression in obese women at term pregnancy".PMID 23948067
  5. Jianjian Shi et al. (2013). "Dissecting the roles of ROCK isoforms in stress-induced cell detachment".PMID 23598717
  6. Jianjian Shi et al. (2013). "Distinct roles for ROCK1 and ROCK2 in the regulation of cell detachment".PMID 23392171
  7. Frances E Lock et al. (2009). "Distinct roles for ROCK1 and ROCK2 in the regulation of keratinocyte differentiation".PMID 19997641
  8. Timo Liebig et al. (2009). "RhoE Is required for keratinocyte differentiation and stratification".PMID 18923151
  9. Ferran Valderrama et al. (2006). "Vaccinia virus-induced cell motility requires F11L-mediated inhibition of RhoA signaling".PMID 16424340
  10. Jasmina Profirovic et al. (2005). "A novel mechanism of G protein-dependent phosphorylation of vasodilator-stimulated phosphoprotein".PMID 16046415