This is a review about Parkin, based on 18 published articles (read how Labome selects the articles), using Parkin antibody in all methods. It is aimed to help Labome visitors find the most suited Parkin antibody.
Parkin synonym: AR-JP; LPRS2; PDJ; PRKN; Parkinson disease (autosomal recessive, juvenile) 2, parkin; parkinson juvenile disease protein 2; parkinson protein 2 E3 ubiquitin protein ligase; parkinson protein 2, E3 ubiquitin protein ligase (parkin)

Knockout validation
Cell Signaling Technologyrabbit polyclonal
  • WB (knockout); mouse; 1:1000; fig 4
In order to investigate PINK1-Mfn2-Parkin-mediated mitophagy in mouse hearts, Cell Signaling Technology Parkin antibody (Cell Signaling, 2132) was used in western blot (knockout) on mouse samples at 1:1000 (fig 4). Science (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (PRK8)
  • WB; mouse
In order to study the effect of parkin deletion and acute knockdown on acetaminophen-induced mitophagy and liver injury in mice, Santa Cruz Biotechnology Parkin antibody (Santa Cruz Biotechnology, SC-32282) was used in western blot on mouse samples. J Biol Chem (2015) ncbi
mouse monoclonal (PRK8)
  • WB; rat; 1:200
  • IC; rat; 1:20
Santa Cruz Biotechnology Parkin antibody (Santa Cruz, sc-32282) was used in western blot on rat samples at 1:200 and in immunocytochemistry on rat samples at 1:20. Neurobiol Dis (2015) ncbi
rabbit polyclonal (H-300)
  • WB; human
In order to explore the parkin-dependent regulation of apoptosis and the turnover of damaged mitochondria in various cell types, Santa Cruz Biotechnology Parkin antibody (Santa Cruz Biotechnology, sc-30130) was used in western blot on human samples. Cell Death Dis (2014) ncbi
mouse monoclonal (PRK8)
  • WB; human
In order to explore the parkin-dependent regulation of apoptosis and the turnover of damaged mitochondria in various cell types, Santa Cruz Biotechnology Parkin antibody (Santa Cruz Biotechnology, sc-32282) was used in western blot on human samples. Cell Death Dis (2014) ncbi
rabbit polyclonal (H-300)
  • IC; chicken
  • WB; chicken
Santa Cruz Biotechnology Parkin antibody (Santa Cruz, sc-30130) was used in immunocytochemistry on chicken samples and in western blot on chicken samples. G3 (Bethesda) (2014) ncbi
mouse monoclonal (PRK8)
  • WB; human
Santa Cruz Biotechnology Parkin antibody (Santa Cruz Biotechnology, sc32282) was used in western blot on human samples. FASEB J (2014) ncbi
rabbit polyclonal (H-300)
  • WB; mouse
In order to study the role of p53 mitochondrial translocation in modulating mitochondrial stress induced by differentiation and the significance for neuronal cell fate, Santa Cruz Biotechnology Parkin antibody (Santa Cruz Biotechnology, sc-30130) was used in western blot on mouse samples. Antioxid Redox Signal (2014) ncbi
mouse monoclonal (PRK8)
  • WB; human
  • WB; rat
In order to study the existance of alpha- and beta-synuclein predomonantly as oligomers in normal neuronal and non-neuronal cells, Santa Cruz Biotechnology Parkin antibody (Santa Cruz, PRK8) was used in western blot on human samples and on rat samples. J Biol Chem (2013) ncbi
EMD Millipore
rabbit polyclonal
  • IP; human; fig 1
EMD Millipore Parkin antibody (Millipore, AB5112) was used in immunoprecipitation on human samples (fig 1). Tumour Biol (2015) ncbi
mouse monoclonal (PRK8)
  • WB; mouse
EMD Millipore Parkin antibody (Millipore, MAB5512) was used in western blot on mouse samples. Diabetologia (2013) ncbi
not indicated
  • WB; human
  • IC; human
Chemicon polyclonal anti-Parkin antibodies were used in western blot to detect Parkin in HEK293 cells as well as in mouse and rat brain cortex, and in immunocytochemistry to stain for Parkin in human dopaminergic neuroblastoma (SH-SY5Y) cell lines.J Biol Chem (2006) ncbi
Abnova
rabbit polyclonal
  • IP; human; fig 1
Abnova Parkin antibody (Abnova, PAB1105) was used in immunoprecipitation on human samples (fig 1). Tumour Biol (2015) ncbi
Abcam
rabbit polyclonal
  • IHC-P; human; 1:300
Abcam Parkin antibody (Abcam, ab55426) was used in immunohistochemistry - paraffin section on human samples at 1:300. Genes Chromosomes Cancer (2013) ncbi
Cell Signaling Technology
rabbit polyclonal
  • WB (knockout); mouse; 1:1000; fig 4
In order to investigate PINK1-Mfn2-Parkin-mediated mitophagy in mouse hearts, Cell Signaling Technology Parkin antibody (Cell Signaling, 2132) was used in western blot (knockout) on mouse samples at 1:1000 (fig 4). Science (2015) ncbi
mouse monoclonal (Prk8)
  • WB; mouse; 1:1000
In order to assess the effect of PINK1-deficiency on mitochondrial quality control in myocytes, Cell Signaling Technology Parkin antibody (Cell Signaling, 4211) was used in western blot on mouse samples at 1:1000. PLoS ONE (2015) ncbi
rabbit polyclonal
  • WB; human; 1:500; fig 2
Cell Signaling Technology Parkin antibody (Cell Signaling, #2132) was used in western blot on human samples at 1:500 (fig 2). Biochim Biophys Acta (2014) ncbi
mouse monoclonal (Prk8)
  • WB; rat; 1:1000
Cell Signaling Technology Parkin antibody (Cell Signaling, Prk8) was used in western blot on rat samples at 1:1000. J Neurochem (2014) ncbi
not indicated
  • WB; human
  • IC; human
Cell Signaling polyclonal anti-Parkin antibodies were used in western blot to detect Parkin in HEK293 cells as well as in mouse and rat brain cortex, and in immunocytochemistry to stain for Parkin in human dopaminergic neuroblastoma (SH-SY5Y) cell lines.J Biol Chem (2006) ncbi
Sigma-Aldrich
mouse monoclonal (PRK8)
  • WB; human; 1:2000
In order to report PINK1 phosphorylates ubiquitin which results in parkin activation, Sigma-Aldrich Parkin antibody (Sigma, PRK8) was used in western blot on human samples at 1:2000. Nature (2014) ncbi
mouse monoclonal (PRK8)
  • IC; African green monkey
  • WB; mouse
Sigma-Aldrich Parkin antibody (Sigma-Aldrich, PRK8) was used in immunocytochemistry on African green monkey samples and in western blot on mouse samples. J Biol Chem (2014) ncbi
GE Healthcare Life Biosciences
not indicatedDharmacon siRNA targeting Parkin was used to identify a functional ubiquitin E3 ligase complex consisting of PD-associated Parkin, PINK1, and DJ-1 to promote degradation of un-/misfolded proteins.J Clin Invest (2009) ncbi
Articles Reviewed
  1. Guohua Gong et al. (2015). "Parkin-mediated mitophagy directs perinatal cardiac metabolic maturation in mice".PMID 26785495
  2. Dieter A Kubli et al. (2015). "PINK1 Is Dispensable for Mitochondrial Recruitment of Parkin and Activation of Mitophagy in Cardiac Myocytes".PMID 26110811
  3. Jessica A Williams et al. (2015). "Chronic Deletion and Acute Knockdown of Parkin Have Differential Responses to Acetaminophen-induced Mitophagy and Liver Injury in Mice".PMID 25752611
  4. Agata Grazia D'Amico et al. (2015). "Expression pattern of parkin isoforms in lung adenocarcinomas".PMID 25656612
  5. Victor S Van Laar et al. (2015). "Glutamate excitotoxicity in neurons triggers mitochondrial and endoplasmic reticulum accumulation of Parkin, and, in the presence of N-acetyl cysteine, mitophagy".PMID 25478815
  6. R A Charan et al. (2014). "Inhibition of apoptotic Bax translocation to the mitochondria is a central function of parkin".PMID 24991765
  7. Daniel Chauss et al. (2014). "Differentiation state-specific mitochondrial dynamic regulatory networks are revealed by global transcriptional analysis of the developing chicken lens".PMID 24928582
  8. Giulia Ambrosi et al. (2014). "Bioenergetic and proteolytic defects in fibroblasts from patients with sporadic Parkinson's disease".PMID 24854107
  9. Fumika Koyano et al. (2014). "Ubiquitin is phosphorylated by PINK1 to activate parkin".PMID 24784582
  10. Gilles Gouspillou et al. (2014). "Increased sensitivity to mitochondrial permeability transition and myonuclear translocation of endonuclease G in atrophied muscle of physically active older humans".PMID 24371120
  11. Joana M Xavier et al. (2014). "Mitochondrial translocation of p53 modulates neuronal fate by preventing differentiation-induced mitochondrial stress".PMID 24329038
  12. Sébastien Brot et al. (2014). "Collapsin response mediator protein 5 (CRMP5) induces mitophagy, thereby regulating mitochondrion numbers in dendrites".PMID 24324268
  13. Bryan Killinger et al. (2014). "Co-administration of betulinic acid and methamphetamine causes toxicity to dopaminergic and serotonergic nerve terminals in the striatum of late adolescent rats".PMID 24151877
  14. W Mandemakers et al. (2013). "Co-regulation of intragenic microRNA miR-153 and its host gene Ia-2 β: identification of miR-153 target genes with functions related to IA-2β in pancreas and brain".PMID 23595248
  15. Ulf Dettmer et al. (2013). "In vivo cross-linking reveals principally oligomeric forms of α-synuclein and β-synuclein in neurons and non-neural cells".PMID 23319586
  16. Marieta I Toma et al. (2013). "PARK2 and PACRG are commonly downregulated in clear-cell renal cell carcinoma and are associated with aggressive disease and poor clinical outcome".PMID 23125027
  17. Hui Xiong et al. (2009). "Parkin, PINK1, and DJ-1 form a ubiquitin E3 ligase complex promoting unfolded protein degradation".PMID 19229105
  18. Ji Won Um et al. (2006). "Parkin ubiquitinates and promotes the degradation of RanBP2".PMID 16332688