This is a review about MSH6, based on 14 published articles (read how Labome selects the articles), using MSH6 antibody in all methods. It is aimed to help Labome visitors find the most suited MSH6 antibody.
MSH6 synonym: GTBP; HNPCC5; HSAP; DNA mismatch repair protein Msh6; G/T mismatch-binding protein; GTMBP; hMSH6; mutS-alpha 160 kDa subunit; p160; sperm-associated protein

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AbD SerotecAbD Serotec MSH6 product
  • immunoprecipitationIn 2001, H E Kleczkowska et al. used AbD Serotec MSH6 antibody to study the role of PCNA in mismatch repair by interaction with hMSH3 and hMSH6 [ncbi , more ].
  • western blotIn 2001, H E Kleczkowska et al. used AbD Serotec MSH6 antibody to study the role of PCNA in mismatch repair by interaction with hMSH3 and hMSH6 [ncbi , more ].
Life Technologies CorporationLife Technologies Corporation MSH6 product
  • immunohistochemistryIn 2008, Kellie S Matthews et al. employed Life Technologies Corporation MSH6 antibody to perform immunohistochemistry in order to show that women diagnosed with endometrial cancer before age 50 may have a mismatch repair deficiency [ncbi , more ].
Santa Cruz BiotechnologySanta Cruz Biotechnology MSH6 product
  • western blotIn 2010, Yiyong Liu et al. used Santa Cruz Biotechnology MSH6 antibody to investigate the roles of MutSalpha and MutLalpha in the regulation of ATR-Chk1 pathway [ncbi , more ].
BD Biosciences
  • ELISAIn 2005, Kevin M Doherty et al. applied BD Biosciences MSH6 antibody to study the interaction between RECQ1 helicase and human mismatch repair factors [ncbi , more ].
  • immunocytochemistryAllen G Schroering et al. applied BD Biosciences MSH6 antibody to study rapid alkylation-induced recruitment of DNA repair proteins to chromosomal DNA within synchronized populations of MMR proficient cells (HeLa MR) after MNNG treatment [ncbi , more ] in 2008 and Ching Tai Lin et al. used BD Biosciences MSH6 antibody to show the effect of VPA and cisplatin on ovarian cancer cells [ncbi , more ].
  • immunohistochemistryIn 2009, Søren A Jensen et al. applied BD Biosciences MSH6 antibody to evaluate the association of MSI and MMR status with outcome and with thymidylate synthase (TS) and dihydropyrimidine dehydrogenase (DPD) expression in colorectal cancer [ncbi , more ]. Barbara I Oberschmid et al. used BD Biosciences MSH6 antibody to study expression of secreted frizzled receptor protein 1 in patients with hyperplastic polyposis coli syndrome. [ncbi , more ] in 2004 and Jens Plaschke et al. used BD Biosciences MSH6 antibody to study the biological function of MSH3 expression [ncbi , more ].
  • western blotIn 2008, Allen G Schroering et al. applied BD Biosciences MSH6 antibody to study rapid alkylation-induced recruitment of DNA repair proteins to chromosomal DNA within synchronized populations of MMR proficient cells (HeLa MR) after MNNG treatment [ncbi , more ]. In 2007, Kwon Tae You et al. applied BD Biosciences MSH6 antibody to perform western blot in order to show that in tumors the tranlation of some NMD-escape PTC-containing mRNAs could be repressed selectively [ncbi , more , fig ]. In 2005, Kevin M Doherty et al. applied BD Biosciences MSH6 antibody to study the interaction between RECQ1 helicase and human mismatch repair factors [ncbi , more ]. Salvatore Cortellino et al. utilized BD Biosciences MSH6 antibody to study the role of MED1 in DNA damage response [ncbi , more ] in 2003 and Hideki Shimodaira et al. used BD Biosciences MSH6 antibody to study the role for the mismatch repair protein PMS2 in the regulation of apoptosis by interacting with the p53-related transcription factor p73 [ncbi , more ]. In 2002, Guido Plotz et al. applied BD Biosciences MSH6 antibody to study the biological function of hMutS alpha on DNA binding [ncbi , more ].
Dako
  • immunohistochemistryIn 2004, Jens Plaschke et al. employed Dako MSH6 antibody to study the biological function of MSH3 expression [ncbi , more ].
Biocare Medical
  • immunohistochemistryIn 2008, Vishes Chhibber et al. applied Biocare Medical MSH6 antibody to perform immunohistochemistry in order to study the frequency of additional mismatch repair protein MSH-6 mutation in sebaceous gland neoplasms [ncbi , more ].
Figure
BD Bioscience anti-hMSH6 was used to perform western blot in order to show that in tumors the tranlation of some NMD-escape PTC-containing mRNAs could be repressed selectively.
MSH6 antibody western blot BD Biosciences
Western Blotting Analysis of NMD-Sensitive and NMD-Escape Proteins Truncated proteins were not detected in NMD-sensitive (hRad50 and hMSH6) genes, whereas wild-type proteins were detected in the cell lines containing the wild-type allele. In the NMD-escape hMSH3 gene, wild-type hMSH3 proteins were detected in the cell lines containing the wild-type allele. However, no truncated proteins of hMSH3 were detected in the cell lines with frameshift mutation. GAPDH was used as a loading control. The single asterisk indicates mutation status of each gene: (−1) denotes a 1-bp deletion in the cMNR, (w) denotes no mutation in the cMNR, and (+1) denotes 1-bp insertion in the cMNR. The double asterisk indicates frameshift mutations of the other coding regions present in both alleles of DLD-1 [38]. The dagger indicates that the expected size of the truncated protein is indicated by the arrow.
Kwon Tae You et al. (2007). "Selective translational repression of truncated proteins from frameshift mutation-derived mRNAs in tumors".PMID 17456004
Articles Reviewed
  1. Yiyong Liu et al. (2010). "Interactions of human mismatch repair proteins MutSalpha and MutLalpha with proteins of the ATR-Chk1 pathway".PMID 20029092
  2. Søren A Jensen et al. (2009). "Microsatellite instability in colorectal cancer and association with thymidylate synthase and dihydropyrimidine dehydrogenase expression".PMID 19154585
  3. Allen G Schroering et al. (2008). "Rapid induction of chromatin-associated DNA mismatch repair proteins after MNNG treatment".PMID 18468964
  4. Kellie S Matthews et al. (2008). "Lynch syndrome in women less than 50 years of age with endometrial cancer".PMID 18448750
  5. Ching Tai Lin et al. (2008). "Valproic acid resensitizes cisplatin-resistant ovarian cancer cells".PMID 18429963
  6. Vishes Chhibber et al. (2008). "MSH-6: extending the reliability of immunohistochemistry as a screening tool in Muir-Torre syndrome".PMID 18065960
  7. Kwon Tae You et al. (2007). "Selective translational repression of truncated proteins from frameshift mutation-derived mRNAs in tumors".PMID 17456004
  8. Kevin M Doherty et al. (2005). "RECQ1 helicase interacts with human mismatch repair factors that regulate genetic recombination".PMID 15886194
  9. Barbara I Oberschmid et al. (2004). "Distinct secreted Frizzled receptor protein 1 staining pattern in patients with hyperplastic polyposis coli syndrome".PMID 15335268
  10. Jens Plaschke et al. (2004). "Loss of MSH3 protein expression is frequent in MLH1-deficient colorectal cancer and is associated with disease progression".PMID 14871813
  11. Salvatore Cortellino et al. (2003). "The base excision repair enzyme MED1 mediates DNA damage response to antitumor drugs and is associated with mismatch repair system integrity".PMID 14614141
  12. Hideki Shimodaira et al. (2003). "Interaction of mismatch repair protein PMS2 and the p53-related transcription factor p73 in apoptosis response to cisplatin".PMID 12601175
  13. Guido Plotz et al. (2002). "hMutSalpha forms an ATP-dependent complex with hMutLalpha and hMutLbeta on DNA".PMID 11809883
  14. H E Kleczkowska et al. (2001). "hMSH3 and hMSH6 interact with PCNA and colocalize with it to replication foci".PMID 11274057