This is a review about MLH1, based on 28 published articles (read how Labome selects the articles), using MLH1 antibody in all methods. It is aimed to help Labome visitors find the most suited MLH1 antibody.
MLH1 synonym: COCA2; FCC2; HNPCC; HNPCC2; hMLH1; mutL homolog 1, colon cancer, nonpolyposis type 2

Santa Cruz Biotechnology
rabbit polyclonal (C-20)
  • IHC-P; human; fig  1
  • WB; human; fig  1
Santa Cruz Biotechnology MLH1 antibody (Santa Cruz Biotechnology, sc-582) was used in immunohistochemistry - paraffin section on human samples (fig  1) and in western blot on human samples (fig  1). PLoS ONE (2015) ncbi
not indicated
  • WB; human
Santa Cruz Biotechnology anti-MLH1 antibody was used for western blot in order to investigate the roles of MutSalpha and MutLalpha in the regulation of ATR-Chk1 pathway.J Biol Chem (2010) ncbi
rabbit polyclonal (C-20)
  • WB; human
Santa Cruz Biotechnology anti-MLH1 antibody (sc-582) was used in western blot to study rapid alkylation-induced recruitment of DNA repair proteins to chromosomal DNA within synchronized populations of MMR proficient cells (HeLa MR) after MNNG treatment.DNA Repair (Amst) (2008) ncbi
not indicated
  • IC; human
Santa Cruz Biotechnology anti-hMLH1 antibody was used in immunocytochemistry (1:25) to demonstrate that heparanase was expressed in lymphocytes of breast cancer PBMC.Neoplasia (2007) ncbi
Invitrogen
mouse monoclonal (ZM001)
  • IHC-P; human; 1:150; tbl 2
In order to assess the use of the estrogen and progesterone receptors as prognostic factors in endometrioid endometrial adenocarcinoma, Invitrogen MLH1 antibody (Zymed, 39-3200) was used in immunohistochemistry - paraffin section on human samples at 1:150 (tbl 2). Gynecol Oncol (2013) ncbi
EMD Millipore
not indicated
  • WB; human
  • IP; human
Calbiochem anti-MLH1 antibody (1:75) was used to perform western blot and immunoprecipitation in order to study the function of small ubiquitin-related modifier (SUMO) 1, SUMO-2/3 and SUMOylation.Hum Reprod (2008) ncbi
BD Biosciences
mouse monoclonal (G168-15)
  • IHC; human; 1:50
In order to compare the immunohistochemical and genetic profiles found in the three types of endometrial carcinomas, BD Biosciences MLH1 antibody (BD, G168-15) was used in immunohistochemistry on human samples at 1:50. Gynecol Oncol (2015) ncbi
mouse monoclonal (G168-15)
  • IHC-P; human
BD Biosciences MLH1 antibody (BD Pharmingen, 550838) was used in immunohistochemistry - paraffin section on human samples. Cancer Genet (2014) ncbi
mouse monoclonal (G168-15)
  • WB; human
In order to explore DNA repair responses in human pluripotent stem cells, BD Biosciences MLH1 antibody (BD Biosciences, 550838) was used in western blot on human samples. J Biol Chem (2014) ncbi
mouse monoclonal (G168-15)
  • IHC-P; human; 1:100
BD Biosciences MLH1 antibody (PharMingen, G168-15) was used in immunohistochemistry - paraffin section on human samples at 1:100. PLoS ONE (2014) ncbi
mouse monoclonal (G168-15)
  • IHC-P; human; 1:30
In order to discuss the diagnosis of colorectal cancer and the need for genetic testing for Lynch Syndrome in young patients, BD Biosciences MLH1 antibody (Biosciences Company, 551092) was used in immunohistochemistry - paraffin section on human samples at 1:30. BMC Surg (2014) ncbi
mouse monoclonal (G168-728)
  • IP; human
  • WB; human
BD Biosciences MLH1 antibody (BD Biosciences, G168-728) was used in immunoprecipitation on human samples and in western blot on human samples. Mol Cancer (2014) ncbi
mouse monoclonal (G168-15)
  • IHC; human
BD Biosciences MLH1 antibody (BD Bio sciences, G-168-15) was used in immunohistochemistry on human samples. Br J Cancer (2014) ncbi
mouse monoclonal (G168-15)
  • IHC-P; human; fig 2
In order to report the first case of filiform polyposis associated with adenomas developed on filiform polyps and invasive colonic adenocarcinoma in a patient without history of inflammatory bowel disease, BD Biosciences MLH1 antibody (BD, G168-15) was used in immunohistochemistry - paraffin section on human samples (fig 2). Pathol Res Pract (2014) ncbi
mouse monoclonal (G168-15)
  • IHC-P; human; 1:30
BD Biosciences MLH1 antibody (BD Biosciences, G168-15) was used in immunohistochemistry - paraffin section on human samples at 1:30. PLoS ONE (2013) ncbi
mouse monoclonal (G168-15)
  • IHC-P; human; 1:25
BD Biosciences MLH1 antibody (BD Pharmingen, G168-15) was used in immunohistochemistry - paraffin section on human samples at 1:25. BMC Gastroenterol (2013) ncbi
mouse monoclonal (G168-15)
  • IHC-P; mouse
BD Biosciences MLH1 antibody (BD Biosciences, G168-15) was used in immunohistochemistry - paraffin section on mouse samples. PLoS Genet (2013) ncbi
not indicated
  • WB; human
BD Biosciences mouse anti-MLH1 antibody was used for western blot in order to investigate the role of Syce1 in the regulation of Synapsis and DNA Repair.PLoS Genet (2009) ncbi
not indicated
  • IHC; human
BD PharMingen monoclonal anti-hMLH1 antibody (clone G168-15) was used in immunohistochemistry to evaluate the association of MSI and MMR status with outcome and with thymidylate synthase (TS) and dihydropyrimidine dehydrogenase (DPD) expression in colorectal cancerBMC Cancer (2009) ncbi
mouse monoclonal (G168-728)
  • IC; human
BD Biosciences anti-MLH1 antibody (554073) was used in immunocytochemistry to study rapid alkylation-induced recruitment of DNA repair proteins to chromosomal DNA within synchronized populations of MMR proficient cells (HeLa MR) after MNNG treatment.DNA Repair (Amst) (2008) ncbi
not indicated
  • IHC; human
BD Pharmingen anti-human mouse MLH1 (clone G168-728) antibody was used to perform immunohistochemistry in order to show that women diagnosed with endometrial cancer before age 50 will have a mismatch repair deficiency.Obstet Gynecol (2008) ncbi
not indicated
  • IC; human
PharMingen anti-hMLH1 antibody was used in immunocytochemistry to show the synergistic cytotoxicity of VPA and cisplatin in ovarian cancer cells.Cancer Sci (2008) ncbi
not indicated
  • IHC; human
Pharmingen mouse monoclonal anti-human MLH-1 (clone G168-15, 1:50) antibody was used to perform immunohistochemistry in order to study the frequency of additional mismatch repair protein MSH-6 mutation in sebaceous gland neoplasms.Mod Pathol (2008) ncbi
mouse monoclonal (G168-15)
  • IC; mouse; 1:50
BD Biosciences MLH1 antibody (BD Pharmingen, G168-1) was used in immunocytochemistry on mouse samples at 1:50. Development (2007) ncbi
not indicated
  • IC; human
Pharmingen monoclonal MLH1 antibody (SWE38) was used in immunocytochemistry to study the role of RAD51C and XRCC3 in genetic recombination and DNA repairJ Biol Chem (2007) ncbi
not indicated
  • WB; human
  • ELISA; human
BD Biosciences mouse anti-MLH1 monoclonal antibody was used in western blot and ELISA to study the interaction between RECQ1 helicase and human mismatch repair factorsJ Biol Chem (2005) ncbi
not indicated
  • IHC; human
Pharmingen monoclonal hMLH1 antibody was used in immunohistochemistry to study the biological features of hereditary nonpolyposis colorectal cancer (HNPCC) in Chinese population.World J Gastroenterol (2005) ncbi
Cell Signaling Technology
mouse monoclonal (4C9C7)
  • WB; human; 1:1000; fig 1g
In order to elucidate the function of TRIM29 in double stranded break repair, Cell Signaling Technology MLH1 antibody (Cell signalling, 3,515) was used in western blot on human samples at 1:1000 (fig 1g). Nat Commun (2015) ncbi
mouse monoclonal (4C9C7)
  • WB; human
Cell Signaling Technology MLH1 antibody (Cell Signaling Technology, 3515) was used in western blot on human samples. elife (2014) ncbi
Articles Reviewed
  1. Yasushi Masuda et al. (2015). "TRIM29 regulates the assembly of DNA repair proteins into damaged chromatin".PMID 26095369
  2. Sanmitra Basu et al. (2015). "A study of molecular signals deregulating mismatch repair genes in prostate cancer compared to benign prostatic hyperplasia".PMID 25938433
  3. Yvette P Geels et al. (2015). "Immunohistochemical and genetic profiles of endometrioid endometrial carcinoma arising from atrophic endometrium".PMID 25773202
  4. Eduardo Vilar et al. (2014). "Role of microsatellite instability-low as a diagnostic biomarker of Lynch syndrome in colorectal cancer".PMID 25432668
  5. Bo Lin et al. (2014). "Human pluripotent stem cells have a novel mismatch repair-dependent damage response".PMID 25012654
  6. Romana Koppensteiner et al. (2014). "Effect of MRE11 loss on PARP-inhibitor sensitivity in endometrial cancer in vitro".PMID 24927325
  7. Jia Chen et al. (2014). "Repair of naturally occurring mismatches can induce mutations in flanking DNA".PMID 24843013
  8. Gian Luca Baiocchi et al. (2014). "Lynch syndrome from a surgeon perspective: retrospective study of clinical impact of mismatch repair protein expression analysis in colorectal cancer patients less than 50 years old".PMID 24533633
  9. Inga Hinrichsen et al. (2014). "Reduced migration of MLH1 deficient colon cancer cells depends on SPTAN1".PMID 24456667
  10. A Malesci et al. (2014). "Molecular heterogeneity and prognostic implications of synchronous advanced colorectal neoplasia".PMID 24434431
  11. Camille Boulagnon et al. (2014). "Filiform polyposis: A benign entity? Case report and literature review".PMID 24315830
  12. Cecilia Egoavil et al. (2013). "Prevalence of Lynch syndrome among patients with newly diagnosed endometrial cancers".PMID 24244552
  13. Jutta Huvila et al. (2013). "Progesterone receptor negativity is an independent risk factor for relapse in patients with early stage endometrioid endometrial adenocarcinoma".PMID 23777659
  14. Marisa Donada et al. (2013). "Management of stage II colon cancer - the use of molecular biomarkers for adjuvant therapy decision".PMID 23446022
  15. Jana Link et al. (2013). "The meiotic nuclear lamina regulates chromosome dynamics and promotes efficient homologous recombination in the mouse".PMID 23382700
  16. Yiyong Liu et al. (2010). "Interactions of human mismatch repair proteins MutSalpha and MutLalpha with proteins of the ATR-Chk1 pathway".PMID 20029092
  17. Ewelina Bolcun-Filas et al. (2009). "Mutation of the mouse Syce1 gene disrupts synapsis and suggests a link between synaptonemal complex structural components and DNA repair".PMID 19247432
  18. Søren A Jensen et al. (2009). "Microsatellite instability in colorectal cancer and association with thymidylate synthase and dihydropyrimidine dehydrogenase expression".PMID 19154585
  19. Petrice W Brown et al. (2008). "Small ubiquitin-related modifier (SUMO)-1, SUMO-2/3 and SUMOylation are involved with centromeric heterochromatin of chromosomes 9 and 1 and proteins of the synaptonemal complex during meiosis in men".PMID 18694876
  20. Allen G Schroering et al. (2008). "Rapid induction of chromatin-associated DNA mismatch repair proteins after MNNG treatment".PMID 18468964
  21. Kellie S Matthews et al. (2008). "Lynch syndrome in women less than 50 years of age with endometrial cancer".PMID 18448750
  22. Ching Tai Lin et al. (2008). "Valproic acid resensitizes cisplatin-resistant ovarian cancer cells".PMID 18429963
  23. Vishes Chhibber et al. (2008). "MSH-6: extending the reliability of immunohistochemistry as a screening tool in Muir-Torre syndrome".PMID 18065960
  24. Thérèse Rachell Theodoro et al. (2007). "Heparanase expression in circulating lymphocytes of breast cancer patients depends on the presence of the primary tumor and/or systemic metastasis".PMID 17603633
  25. Yuki Takada et al. (2007). "Mammalian Polycomb Scmh1 mediates exclusion of Polycomb complexes from the XY body in the pachytene spermatocytes".PMID 17215307
  26. Yilun Liu et al. (2007). "Role of RAD51C and XRCC3 in genetic recombination and DNA repair".PMID 17114795
  27. Kevin M Doherty et al. (2005). "RECQ1 helicase interacts with human mismatch repair factors that regulate genetic recombination".PMID 15886194
  28. Ding Cun Luo et al. (2005). "Clinicopathological and molecular genetic analysis of HNPCC in China".PMID 15786548