This is a review about MEK1, based on 44 published articles (read how Labome selects the articles), using MEK1 antibody in experiments. It is aimed to help Labome visitors find the most suited MEK1 antibody.
MEK1 synonym: CFC3; MAPKK1; MEK1; MKK1; PRKMK1; ERK activator kinase 1; MAPK/ERK kinase 1; MAPKK 1; MEK 1; dual specificity mitogen-activated protein kinase kinase 1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

  • Millipore polyclonal anti-MEK1 antibody was used to carry out western blot assays in order to investigate the molecular mechanisms of GRASP55 and GRASP65 in Golgi cisternal stacking. (catalog: 07-641) ncbi, more
  • suppliernumreagent linkcited catalog
    Santa Cruz Biotechnology11MEK1 antibodysc-219
    EMD Millipore4MEK1 antibody07-641
    Enzo Life Sciences1MEK1 antibody
    Cell Signaling Technology31MEK1 antibody9121S
    BD Biosciences4MEK1 antibody610122
    Dako1
    Table 1. MEK1 antibody western blot.
  • Upstate Technologies MAPKK1 antibody was used in western blot in order to demonstrate that celastrol inhibits lethal toxin-induced death of RAW264.7 murine macrophagesncbi, more
  • Calbiochem anti-phosphorylated-MEK-1 antibody was used in western blot to study up-regulation of endothelial nitric-oxide synthase promoter in the phosphatidylinositol 3-kinase gamma /Janus kinase 2/MEK-1-dependent pathway.ncbi, more
  • Upstate Biotechnology anti-MEK-1 antibody was used in western blot to study up-regulation of endothelial nitric-oxide synthase promoter in the phosphatidylinositol 3-kinase gamma /Janus kinase 2/MEK-1-dependent pathway.ncbi, more
  • Santa Cruz Biotechnology rabbit polyclonal antibody against MEK1/2 was used in western blot to investigate the phosphatidylinositol 3-kinase-PKC-zeta-MEK-ERK signaling pathway mediated by Kaposi's sarcoma-associated herpesvirus early during infection of target cells. (catalog: sc-219) ncbi, more
  • Santa Cruz Biotechnology anti-MEK1 antibody was used to perform western blot and flow cytometry in order to study the signaling mechanism in the resistance of T cells from asthmatic patients against suppression by TGFbeta and IL-10.ncbi, more
  • suppliernumreagent link
    Santa Cruz Biotechnology1MEK1 antibody
    Cell Signaling Technology2MEK1 antibody
    Table 2. MEK1 antibody flow cytometry.
  • Santa Cruz Biotechnology anti-human MEK-1 (H-8) monoclonal antibody was used to perform western blot in order to study the role of calmodulin kinase in ligand-independent EGFR degradation in pregnancy.ncbi, more
  • Santa Cruz anti-phospho-MEK1/2 antibody (S217/221) and anti-MEK1/2 antibody (H8) were used in western blot and ELISA to study the potential binding partners to Cot and their region of association by separating proteins that were pulled-down with various Cot constructs following immunoprecipiation in human HEK293 cells and Hela cells.ncbi, more
  • SCBT MEK1 polyclonal antibody was used to investigate the role of GIT1 in ERK1/2 activation during focal adhesionsncbi, more
  • Santa Cruz Biotechnology anti-MEK antibody was used in western blot to study the immortalization of bovine lens epithelial cells by human telomerase reverse transcriptasencbi, more
  • Santa Cruz Biotechnology mouse monoclonal anti-MEK1 antibody was used in western blot to study a New Negative Regulator of A-Raf Kinase, trihydrophobin 1.ncbi, more
  • suppliernumreagent link
    Santa Cruz Biotechnology1MEK1 antibody
    Cell Signaling Technology1MEK1 antibody
    Table 3. MEK1 antibody immunocytochemistry.
  • Santa Cruz Biotechnology anti-Mek-1 antibody was used in thyroid TAD-2 cells and the hepatoma cell line Hep3B and in immunoprecipitation to study the interaction of calcium/calmodulin-dependent protein kinase II with Raf-1 and the effect of it on integrin-stimulated ERK activation.ncbi, more
  • Santa Cruz Biotechnology anti-MEK antibody was used in western blot to study whether RKIP functions as a suppressor of prostate cancer metastasis in human prostate cancer cells.ncbi, more
  • Santa Cruz Biotechnology anti-MEK-1 polyclonal antibody was used in western blot to study the regulation of activity of the human tissue factor pathway inhibitor-2 promoter by the ERK/MAPK pathway.ncbi, more
  • Santa Cruz MEK1 antibody was used in western blot to study the downregulation of the Ras-mitogen-activated protein kinase pathway by the EphB2 receptor tyrosine kinase.ncbi, more
  • Santa Cruz Biotechnology rabbit polyclonal Mek1 antibody was used in western blot to study Rap1 signaling activation in endosomes by nerve growth factorncbi, more
  • suppliernumreagent link
    Santa Cruz Biotechnology1MEK1 antibody
    Cell Signaling Technology1MEK1 antibody
    Table 4. MEK1 antibody immunoprecipitation.
  • StressGen Biotechnologies anti-MEK1 monoclonal antibody was used in western blot to study SP/NK1R-induced cell death mediated by a MAP kinase activation pathway involving Raf-1, MEK2, and extracellular signal-regulated protein kinase 2 (ERK2)ncbi, more
  • Transduction Laboratories anti-MEK1 was used to perform western blot in order to show that MEK1 or MEK2 activation is sufficient for metastatic tumor formation.ncbi, more , figure 1
  • Figure 1
    MEK1 antibody western blot BD Biosciences
    MEK1 antibody western blot BD Biosciences
    Immunoblot analysis of MEK1, MEK2 and phospho-MEK1/2 expression in the human colon carcinoma cell lines HCT116, HT-29 and SW480, and in the breast adenocarcinoma line MDA-MB-231. Lysates were prepared from cells deprived of serum for 24 h (c), restimulated with serum for 5 min (+), or proliferating exponentially (exp).
    Laure Voisin et al. (2008). "Activation of MEK1 or MEK2 isoform is sufficient to fully transform intestinal epithelial cells and induce the formation of metastatic tumors".PMID 19014680
  • BD mouse anti-MEK1 antibody (catalog no:610122) was used in western blot to combine computational simulations, experimental testing of simulation results, and finally reverse engineering of a protein interaction network to define potential therapeutic strategies for de novo trastuzumab resistant breast cancer (catalog: 610122) ncbi, more
  • Transduction Laboratories monoclonal anti-MEK1 antibody was used in western blot to detect MEK1 in A549 cells.ncbi, more
  • Transduction Laboratories phospho-MEK1 antibody was used in western blot to study the downregulation of the Ras-mitogen-activated protein kinase pathway by the EphB2 receptor tyrosine kinase.ncbi, more
  • Cell Signaling anti-phospho-MEK1/2 was used to perform western blot in order to show that MEK1 or MEK2 activation is sufficient for metastatic tumor formation.ncbi, more , figure 2
  • Figure 2
    MEK1 antibody western blot Cell Signaling Technology
    MEK1 antibody western blot Cell Signaling Technology
    Immunoblot analysis of MEK1, MEK2 and phospho-MEK1/2 expression in the human colon carcinoma cell lines HCT116, HT-29 and SW480, and in the breast adenocarcinoma line MDA-MB-231. Lysates were prepared from cells deprived of serum for 24 h (c), restimulated with serum for 5 min (+), or proliferating exponentially (exp).
    Laure Voisin et al. (2008). "Activation of MEK1 or MEK2 isoform is sufficient to fully transform intestinal epithelial cells and induce the formation of metastatic tumors".PMID 19014680
  • Cell Signaling anti-p-MEK1/2 (no. 9121S) antibody was used in Western blot to study the biological function of TRAIL induced apoptosis in human colon cells. (catalog: 9121S) ncbi, more
  • Cell Signaling Technology rabbit antibody against the phosphorylated form of MEK1/2 (Ser217/221 phospho-MEK1/2) was used in western blot to study the role for PI 3-kinase in HHV-8 entry into the target cells and the role for PKC-zeta, MEK, and ERK at a post-viral entry stage of infection.ncbi, more
  • Cell Signaling Technology anti-MEK1 antibody was used to perform flow cytometry and immunochemistry in order to study the signaling mechanism in the resistance of T cells from asthmatic patients against suppression by TGFbeta and IL-10.ncbi, more
  • Cell Signaling Technology anti-MEK1/2 antibody was used to perform western blot and flow cytometry in order to study the signaling mechanism in the resistance of T cells from asthmatic patients against suppression by TGFbeta and IL-10.ncbi, more
  • Cell Signaling Technology anti-MEK1 and anti-phospho-MEK1 antibodies were used in western blot and immunoprecipitation in order to study the role of Beclin 1 in the regulation of autophagy.ncbi, more
  • Cell Signaling anti-phospho-MEK antibody was used for western blot in order to investigate the role of BRAF in the regulation of RAF-MEK signaling pathway.ncbi, more
  • suppliernumreagent link
    Cell Signaling Technology1MEK1 antibody
    Table 5. MEK1 antibody EMSA.
  • Cell Signaling anti-total MEK and anti-phospho-MEK antibodies were used in western blot to test the hypothesis that additional RAS mutations outside of codons 12, 13, and 61 may account for oncogenesis in myeloid malignancies.ncbi, more
  • Cell Signaling anti-p-MEK1 antibody was used in western blot to study the role for Hsp72 in suppression of the oncogene-induced senescence.ncbi, more
  • Cell Signaling Technology MEK1/2 antibody was used in western blot in order to show that Hsp90 co-chaperone CDC37 can maintain the oncogenic protein kinase clients.ncbi, more
  • Cell Signaling Technology anti-Mek1/2 antibody was used to perform western blot in order to study the role of Raf-1 in glioma formation.ncbi, more
  • Cell Signaling Technology ERK phospho-MEK antibody was used in western blot in order to study the role of PAX4 in the beta-cell differentiation of human embryonic stem cellsncbi, more
  • suppliernumreagent link
    Cell Signaling Technology1MEK1 antibody
    Table 6. MEK1 antibody immunohistochemistry.
  • Cell Signaling Technology anti-MEK1 and anti-phospho-MEK1 (Ser217/221) antibodies were used in human 1HAEo cell line and in western blot to study the link between the PI3K-Akt pathway and the cigarette smoke (CS) stimulated epidermal growth factor receptor-mediated FRA-1 induction in non-oncogenic HBE cells.ncbi, more
  • Cell Signaling Technology anti-phospho-MEK antibody was used in western blot to study heterogeneous de novo missense mutations in three genes within the mitogen-activated protein kinase (MAPK) pathway.ncbi, more
  • Cell Signaling anti-MEK1/2 was used in western blot to detect MEK expression and MEK phosphorylation status in human A549 lung adenocarcinoma cells.ncbi, more
  • Cell Signaling anti-phospho-MEK1 antibody was used in western blot to detect phospho-MEK1 in HT-29 cells treated with NMT1-1 siRNA or NMT2-4 siRNA.ncbi, more
  • Cell Signaling anti-phospho-MEK1/2 (Ser-217/221) antibody was used to investigate the activation of Erk1/2 by intracellular M. lepraencbi, more
  • suppliernumreagent link
    Santa Cruz Biotechnology1MEK1 antibody
    Table 7. MEK1 antibody ELISA.
  • Cell Signaling pMEK1/2 polyclonal antibody was used to investigate the role of GIT1 in ERK1/2 activation during focal adhesionsncbi, more
  • Cell Signaling Technology polyclonal anti-phospho-MEK1/2 and anti-MEK1/2 antibodies were used in human umbilical vein endothelial cells (HUVECs) and in western blot to study the effect of enhanced p38 MAPK activity on endothelial cell function.ncbi, more
  • Cell Signaling Technology phospho-MEK1/2 (S217/S221) antibody was used in western blot to study interleukin 7 effects on T cell acute lymphoblastic leukemia cellsncbi, more
  • Cell Signaling rabbit anti-MEK1/2 antibody was used in western blot to study a novel BASH N terminus-associated protein (BNAS2).ncbi, more
  • Cell Signaling Technology anti-MEK1/2 antibody was used in western blot to study SP/NK1R-induced cell death mediated by a MAP kinase activation pathway involving Raf-1, MEK2, and extracellular signal-regulated protein kinase 2 (ERK2)ncbi, more
  • Cell Signaling Technology anti-p-ERK1 antibody (anti-phosphorylated Thr202/204) was used in western blot to study a New Negative Regulator of A-Raf Kinase, trihydrophobin 1.ncbi, more
  • Cell Signaling Technology anti-p-MEK antibody was used in western blot to study whether RKIP functions as a suppressor of prostate cancer metastasis in frozen sections from human tissue.ncbi, more
  • Cell Signaling Technology anti-MEK1 and phospho-MEK1 antibody was used in western blot to study the expression of hypertonicity-induced aquaporin-1 (AQP1) which is mediated by the activation of MAPK pathways and hypertonicity-responsive element in the AQP1 gene.ncbi, more
  • New England Biolabs anti-phospho-MEK1/2 antibody was used in western blot to study the phosphorylation of several members of the MAPK pathways.ncbi, more
  • Cell Signaling Technology rabbit anti-phospho-MEK1/2 (Ser-217/Ser-221) antibody was used in western blot to study the role of RSK in signaling chemokine responses and synthesis in astrocytes.ncbi, more
  • New England Biolabs anti-phospho-MEK1/2 antibody was used in human astrocytoma cell line 1321N1 and in western blot to study the ability of lead to activate the MAPK cascade and the role of PKC in this signal transduction pathway.ncbi, more
  • Cell Signaling Technologies phospho-MEK1 antibody was used in western blot to study the downregulation of the Ras-mitogen-activated protein kinase pathway by the EphB2 receptor tyrosine kinase.ncbi, more
  • NEB PhosphoPlus MEK-1/2 (Ser217/221) antibody kit was used in western blot to identify arsenic trioxide as a potent inhibitor of the interaction of SMRT corepressor with its transcription factor partners such as T3Rs and RARs in human acute promyelocytic leukemia.ncbi, more
  • New England Biolabs anti-MEK1/2 and anti-phospho-MEK1/2 antibodies were used in western blot to study ERK2 nuclear shuttling in RBL-2H3 cells.ncbi, more
  • Cell Signaling Technology phospho-MEK1/2 (catalog no. 91215) and MEK1/2 (catalog no. 9122) antibodies were used in western blot to study the role of CD45 in monocytic cells.ncbi, more
  • New England Biolabs rabbit polyclonal antibody recognizing the phosphorylated forms of MEK (anti-phospo-Ser217/221 MEK1/2) was used in western blot to study borna disease virus persistent infection in the central nervous system.ncbi, more
Dako
  • Cell Signaling Technology rabbit polyclonal anti-MAP kinase p44/p42 antibody was used to perform westn blot in order to investigate the relations between TGFbeta1 and TNFa.ncbi, more
Articles Reviewed
  1. Qiaoling Liang et al. (2010). "IL-2 and IL-4 stimulate MEK1 expression and contribute to T cell resistance against suppression by TGF-beta and IL-10 in asthma".PMID 20926789
  2. Yi Xiang et al. (2010). "GRASP55 and GRASP65 play complementary and essential roles in Golgi cisternal stacking".PMID 20083603
  3. Jianrong Wang et al. (2009). "A non-canonical MEK/ERK signaling pathway regulates autophagy via regulating Beclin 1".PMID 19520853
  4. Xiaoying Liu et al. (2009). "Transforming growth factor beta1-mediated activation of the smooth muscle alpha-actin gene in human pulmonary myofibroblasts is inhibited by tumor necrosis factor-alpha via mitogen-activated protein kinase kinase 1-dependent induction of the Egr-1 transcr".PMID 19261809
  5. Christine A Pratilas et al. (2009). "(V600E)BRAF is associated with disabled feedback inhibition of RAF-MEK signaling and elevated transcriptional output of the pathway".PMID 19251651
  6. Ozgur Sahin et al. (2009). "Modeling ERBB receptor-regulated G1/S transition to find novel targets for de novo trastuzumab resistance".PMID 19118495
  7. Jeffrey W Tyner et al. (2009). "High-throughput sequencing screen reveals novel, transforming RAS mutations in myeloid leukemia patients".PMID 19075190
  8. Laure Voisin et al. (2008). "Activation of MEK1 or MEK2 isoform is sufficient to fully transform intestinal epithelial cells and induce the formation of metastatic tumors".PMID 19014680
  9. Vladimir L Gabai et al. (2009). "Heat shock protein Hsp72 controls oncogene-induced senescence pathways in cancer cells".PMID 19001088
  10. J R Smith et al. (2009). "Silencing the cochaperone CDC37 destabilizes kinase clients and sensitizes cancer cells to HSP90 inhibitors".PMID 18931700
  11. Tushar B Deb et al. (2008). "Pregnancy-upregulated nonubiquitous calmodulin kinase induces ligand-independent EGFR degradation".PMID 18562482
  12. Yelena Lyustikman et al. (2008). "Constitutive activation of Raf-1 induces glioma formation in mice".PMID 18472967
  13. Takashi Nakakuki et al. (2008). "Topological analysis of MAPK cascade for kinetic ErbB signaling".PMID 18335053
  14. Binhui Wu et al. (2008). "Proteomics analysis of immunoprecipitated proteins associated with the oncogenic kinase cot".PMID 18319612
  15. Sarah Chapelsky et al. (2008). "Inhibition of anthrax lethal toxin-induced cytolysis of RAW264.7 cells by celastrol".PMID 18183301
  16. Qin Zhang et al. (2006). "A Phosphatidylinositol 3-kinase-regulated Akt-independent signaling promotes cigarette smoke-induced FRA-1 expression".PMID 16490785
  17. Pablo Rodriguez-Viciana et al. (2006). "Germline mutations in genes within the MAPK pathway cause cardio-facio-cutaneous syndrome".PMID 16439621
  18. Anna Moshnikova et al. (2006). "The growth and tumor suppressor NORE1A is a cytoskeletal protein that suppresses growth by inhibition of the ERK pathway".PMID 16421102
  19. Charles E Ducker et al. (2005). "Two N-myristoyltransferase isozymes play unique roles in protein myristoylation, proliferation, and apoptosis".PMID 16123142
  20. Nikos Tapinos et al. (2005). "Insights into regulation of human Schwann cell proliferation by Erk1/2 via a MEK-independent and p56Lck-dependent pathway from leprosy bacilli".PMID 15967991
  21. Guoyong Yin et al. (2005). "GIT1 is a scaffold for ERK1/2 activation in focal adhesions".PMID 15923189
  22. Juan Wang et al. (2005). "Human telomerase reverse transcriptase immortalizes bovine lens epithelial cells and suppresses differentiation through regulation of the ERK signaling pathway".PMID 15849192
  23. Meghan E McMullen et al. (2005). "Activation of p38 has opposing effects on the proliferation and migration of endothelial cells".PMID 15790570
  24. Konstantinos G Drosopoulos et al. (2005). "Transformation by oncogenic RAS sensitizes human colon cells to TRAIL-induced apoptosis by up-regulating death receptor 4 and death receptor 5 through a MEK-dependent pathway".PMID 15757891
  25. Joao T Barata et al. (2004). "Activation of PI3K is indispensable for interleukin 7-mediated viability, proliferation, glucose use, and growth of T cell acute lymphoblastic leukemia cells".PMID 15353558
  26. Yasuhiro Imamura et al. (2004). "Identification and characterization of a novel BASH N terminus-associated protein, BNAS2".PMID 15087455
  27. Susana Castro-Obregon et al. (2004). "Alternative, nonapoptotic programmed cell death: mediation by arrestin 2, ERK2, and Nur77".PMID 14769794
  28. Weicheng Liu et al. (2004). "Trihydrophobin 1 is a new negative regulator of A-Raf kinase".PMID 14684750
  29. Maddalena Illario et al. (2003). "Calcium/calmodulin-dependent protein kinase II binds to Raf-1 and modulates integrin-stimulated ERK activation".PMID 12954639
  30. Zheng Fu et al. (2003). "Effects of raf kinase inhibitor protein expression on suppression of prostate cancer metastasis".PMID 12813171
  31. Fuminori Umenishi et al. (2003). "Hypertonicity-induced aquaporin-1 (AQP1) expression is mediated by the activation of MAPK pathways and hypertonicity-responsive element in the AQP1 gene".PMID 12600999
  32. Pramod P Naranatt et al. (2003). "Kaposi's sarcoma-associated herpesvirus induces the phosphatidylinositol 3-kinase-PKC-zeta-MEK-ERK signaling pathway in target cells early during infection: implications for infectivity".PMID 12502866
  33. Christina Kast et al. (2003). "The ERK/MAPK pathway regulates the activity of the human tissue factor pathway inhibitor-2 promoter".PMID 12446683
  34. Diane Lejeune et al. (2002). "Interleukin-22 (IL-22) activates the JAK/STAT, ERK, JNK, and p38 MAP kinase pathways in a rat hepatoma cell line. Pathways that are shared with and distinct from IL-10".PMID 12087100
  35. Ye Zhang et al. (2002). "RANTES-mediated chemokine transcription in astrocytes involves activation and translocation of p90 ribosomal S6 protein kinase (RSK)".PMID 11893739
  36. Jianguo Gu et al. (2002). "Laminin-10/11 and fibronectin differentially prevent apoptosis induced by serum removal via phosphatidylinositol 3-kinase/Akt- and MEK1/ERK-dependent pathways".PMID 11891225
  37. Hailing Lu et al. (2002). "Inorganic lead activates the mitogen-activated protein kinase kinase-mitogen-activated protein kinase-p90(RSK) signaling pathway in human astrocytoma cells via a protein kinase C-dependent mechanism".PMID 11861786
  38. S Elowe et al. (2001). "Downregulation of the Ras-mitogen-activated protein kinase pathway by the EphB2 receptor tyrosine kinase is required for ephrin-induced neurite retraction".PMID 11585923
  39. S H Hong et al. (2001). "Arsenic trioxide is a potent inhibitor of the interaction of SMRT corepressor with Its transcription factor partners, including the PML-retinoic acid receptor alpha oncoprotein found in human acute promyelocytic leukemia".PMID 11585900
  40. C Wu et al. (2001). "Nerve growth factor activates persistent Rap1 signaling in endosomes".PMID 11466412
  41. T Furuno et al. (2001). "Nuclear shuttling of mitogen-activated protein (MAP) kinase (extracellular signal-regulated kinase (ERK) 2) was dynamically controlled by MAP/ERK kinase after antigen stimulation in RBL-2H3 cells".PMID 11254696
  42. E L Deszo et al. (2001). "CD45 negatively regulates monocytic cell differentiation by inhibiting phorbol 12-myristate 13-acetate-dependent activation and tyrosine phosphorylation of protein kinase Cdelta".PMID 11124968
  43. A Hans et al. (2001). "Borna disease virus persistent infection activates mitogen-activated protein kinase and blocks neuronal differentiation of PC12 cells".PMID 11073944
  44. K Cieslik et al. (2001). "Up-regulation of endothelial nitric-oxide synthase promoter by the phosphatidylinositol 3-kinase gamma /Janus kinase 2/MEK-1-dependent pathway".PMID 11042169