This is a review about H3, based on 42 published articles (read how Labome selects the articles), using H3 antibody in all methods. It is aimed to help Labome visitors find the most suited H3 antibody.
H3 synonym: H3.2; H3/M; H3F2; H3FM; H3FN; H3 histone family, member M; H3 histone, family 2; histone 2, H3c; histone H3/m

Santa Cruz Biotechnology
goat polyclonal (C-16)
  • WB; human; fig 7.a,b
In order to study the effects of cocaine on HIV-infected myeloid cells, Santa Cruz Biotechnology H3 antibody (Santa Cruz, SC-8654) was used in western blot on human samples (fig 7.a,b). Virology (2015) ncbi
goat polyclonal (C-16)
  • IHC-P; human
  • ChIP; human
Santa Cruz Biotechnology H3 antibody (Santa Cruz, sc-8654) was used in immunohistochemistry - paraffin section on human samples and in chromatin immunoprecipitation on human samples. Nucleic Acids Res (2015) ncbi
goat polyclonal (C-16)
  • WB; human
In order to demonstrate that SNW1 and PRPF8 are essential for sister chromatid cohesion in human cells, Santa Cruz Biotechnology H3 antibody (Santa Cruz, sc-8654) was used in western blot on human samples. EMBO J (2014) ncbi
goat polyclonal (C-16)
  • WB; human
In order to study the role of p19INK4d in the repair machinery for DNA damage, Santa Cruz Biotechnology H3 antibody (Santa Cruz Biotechnology, sc-8654-R) was used in western blot on human samples. Mol Cell Biochem (2015) ncbi
rabbit polyclonal (FL-136)
  • WB; human
Santa Cruz anti-H3 antibody was used to perform western blot in order to study the expression of Aurora A kinase substrates in estrogen-induced ectopic uterine stem cell tumors.Adv Exp Med Biol (2008) ncbi
Invitrogen
rabbit polyclonal
  • WB; human
In order to show that silencing of p21(Cip1) results in MEP50-mediated enhancement of keratinocyte proliferation and survival, Invitrogen H3 antibody (Thermo Fisher Scientific, PA5-27039) was used in western blot on human samples. J Biol Chem (2015) ncbi
rabbit polyclonal
  • ChIP; mouse
In order to elucidate the role of E-cadherin in pluripotency, mESC differentiation, and tumor metastasis, Invitrogen H3 antibody (Pierce, PA5-27029) was used in chromatin immunoprecipitation on mouse samples. Stem Cells (2014) ncbi
Active Motif
rabbit polyclonal
  • WB; human
  • ChIP; human
Active Motif anti-H3K9Ac was used to perform western blot and ChIP assay in order to show that circadian clock could be regulated by JARID1a-induced CLOCK-BMAL1 activation.Science (2011) ncbi
rabbit polyclonal
  • WB; human
  • ChIP; human
Active Motif anti-H3K4me3 was used to perform western blot and ChIP assay in order to show that circadian clock could be regulated by JARID1a-induced CLOCK-BMAL1 activation.Science (2011) ncbi
EMD Millipore
not indicated
  • IP; human
Upstate anti-H3K4 antibody was used to perform RNA immunoprecipitation in order to analyze histone methylation at transcription start sites in human-specific prefrontal neurons of brain.PLoS Biol (2012) ncbi
rabbit polyclonal
  • WB; human
Millipore anti-H3 antibody was used to perform western blot in order to study the DNA damage and response by UV.PLoS Biol (2011) ncbi
not indicated
  • WB; human
  • ChIP; human
Upstate anti-H3K9Ac was used to perform western blot and ChIP assay in order to show that circadian clock could be regulated by JARID1a-induced CLOCK-BMAL1 activation.Science (2011) ncbi
not indicated
  • WB; human
Millipore histone H3 Ser10 antibody was used to perform western blot in order to investigate the relations between SGI-1776 and chronic lymphocytic leukemiaBlood (2009) ncbi
not indicated
  • WB; human
Upstate anti-acetyl H3 antibody was used in western blot in order to explain that PDCD5 can maintain the histone acetyltransferase activity of the Tip60 protein and promote DNA damage-induced apoptosis as a Tip60 coactivator.Neoplasia (2009) ncbi
not indicated
  • IP; human
Upstate Biotechnologies anti-phospho-H3 antibody was used to do immunoprecipitation in order to study the role of MERIT40 in BRCA1 localization and DNA damage repairGenes Dev (2009) ncbi
not indicated
  • IHC; human
Upstate anti-phosphohistone H3 antibody was used to perform immunohistochemistry in order to study the role of mitochondrial flavoprotein apoptosis inducing factor.PLoS ONE (2009) ncbi
not indicated
  • ChIP; human
Upstate anti-H3 antibody was used to perform ChIP assays in order to study the association between the papillomavirus E2 proteins, the host Brd4 protein and the transcriptionally active cellular genes.J Virol (2009) ncbi
not indicated
  • IP; human
Upstate anti-Ac-H3 antibody was used to perform immunoprecipitation in order to study the association between ING4 and the activity of NF-kappa B molecules bound with target gene promoters.Mol Cell Biol (2008) ncbi
not indicated
  • WB; human
Upstate Biotechnology H3K4me1 antibody was used in western blot in order to demonstrate that AIRE binds to histone H3 through its first PHD finger (AIRE PHD1)EMBO Rep (2008) ncbi
not indicated
  • WB; human
Upstate Biotechnology H3K9me3 antibody was used in western blot in order to demonstrate that AIRE binds to histone H3 through its first PHD finger (AIRE PHD1)EMBO Rep (2008) ncbi
not indicated
  • WB; human
  • IP; human
Upstate Biotechnology AcH3 (K9K14), PAcH3 (K14S10), PH3 (S10) antibodies were used in western blot and immunoprecipitation to demonstrate that neural agrin-dependent reprogramming of myonuclei involves chromatin remodelling, histone hyperacetylation, and histone hyperphosphorylation.EMBO J (2007) ncbi
not indicated
  • ChIP; human
Upstate anti-H3 K9 acetylation antibody was used in ChIP assay to study the molecular mechanism of gene activation and repression by Pax6in mouse alphaTN4-1 cells.J Mol Biol (2005) ncbi
Abcam
rabbit polyclonal
  • ChIP; human
Abcam anti-H3 was used to perform chromatin immunoprecipitation in order to study the role of deacetylase inhibitors to ING2 subunit from the Sin3 complex.Chem Biol (2010) ncbi
not indicated
  • WB; human
Abcam anti-H3 antibody 1:25,000 was used for western blot in order to investigate the roles of H3K9Ac and H3K56Ac in the regulation of DNA-damage-responsive in human cellsEMBO J (2009) ncbi
not indicated
  • WB; human
Abcam rabbit polyclonal anti-H3 antibody was used to carry out western blot analysis in order to investigate the role of DNA polymerase mu in physiological hematopoietic development.PLoS Genet (2009) ncbi
not indicated
  • ChIP; human
Abcam anti-H3 antibody was used to perform ChIP assays in order to study the function of snail1 in controlling bone mass during osteoblast differentiation.EMBO J (2009) ncbi
not indicated
  • WB; human
Abcam H3K4me3 antibody was used in western blot in order to demonstrate that AIRE binds to histone H3 through its first PHD finger (AIRE PHD1)EMBO Rep (2008) ncbi
rabbit polyclonal
  • WB; human
Abcam H3 antibody was used in western blot in order to demonstrate that AIRE binds to histone H3 through its first PHD finger (AIRE PHD1)EMBO Rep (2008) ncbi
BioLegend
not indicated
  • FC; human
Biolegend anti-H3 (pS28) was used in 0.5ug/ml to perform mass cytometry in order to show that different stimulation effect of human hematopoietic cells could be analysized by single-cell mass cytometry.Science (2011) ncbi
Sigma-Aldrich
mouse monoclonal (AH3-120)
  • WB; human; 1:500
Sigma-Aldrich H3 antibody (Sigma Aldrich, H0913) was used in western blot on human samples at 1:500. Biotechnol Bioeng (2015) ncbi
rabbit polyclonal
  • WB; human; 1:5000
Sigma-Aldrich H3 antibody (Sigma Aldrich, H0164) was used in western blot on human samples at 1:5000. Biotechnol Bioeng (2015) ncbi
rat monoclonal (HTA28)
  • IHC; mouse; 1:50; fig 5
In order to test if extracellular signals orient the mitotic spindle of cells in the spinal cord, Sigma-Aldrich H3 antibody (Sigma, HTA28) was used in immunohistochemistry on mouse samples at 1:50 (fig 5). Nat Commun (2015) ncbi
rabbit polyclonal
  • IHC; zebrafish; 1:200; fig 6
Sigma-Aldrich H3 antibody (Sigma, H0164) was used in immunohistochemistry on zebrafish samples at 1:200 (fig 6). Development (2015) ncbi
rabbit polyclonal
  • IHC; fruit fly; 1:2000
In order to assess the developmental origin of subcompartments in axons and dendrites, Sigma-Aldrich H3 antibody (Sigma, H0164) was used in immunohistochemistry on fruit fly samples at 1:2000. Development (2015) ncbi
rat monoclonal (HTA28)
  • IHC; mouse
Sigma-Aldrich H3 antibody (Sigma-Aldrich, H9908) was used in immunohistochemistry on mouse samples. PLoS ONE (2014) ncbi
rabbit polyclonal
  • WB; domestic silkworm
Sigma-Aldrich H3 antibody (Sigma-Aldrich, H0164) was used in western blot on domestic silkworm samples. Insect Biochem Mol Biol (2014) ncbi
mouse monoclonal (AH3-120)
  • IC; human; 1:200
Sigma-Aldrich H3 antibody (Sigma, H0913) was used in immunocytochemistry on human samples at 1:200. Cryobiology (2014) ncbi
mouse monoclonal (AH3-120)
  • IHC-P; human; fig 2
  • ChIP; human; 2-5 ug/ChIP; fig 2
In order to investigate the role of repressor element 1-silencing transcription factor in neurodegeneration during ageing, Sigma-Aldrich H3 antibody (Sigma, H0913) was used in immunohistochemistry - paraffin section on human samples (fig 2) and in chromatin immunoprecipitation on human samples at 2-5 ug/ChIP (fig 2). Nature (2014) ncbi
rat monoclonal (HTA28)
  • IC; human
In order to identify QARS (encoding glutaminyl-tRNA synthetase [QARS]) mutations in two unrelated families affected by progressive microcephaly, Sigma-Aldrich H3 antibody (Sigma-Aldrich, H9908) was used in immunocytochemistry on human samples. Am J Hum Genet (2014) ncbi
rat monoclonal (HTA28)
  • IC; Planorbella trivolvis; 1:1000
Sigma-Aldrich H3 antibody (Sigma-Aldrich, H9908) was used in immunocytochemistry on Planorbella trivolvis samples at 1:1000. BMC Dev Biol (2014) ncbi
rat monoclonal (HTA28)
  • IHC-F; mouse; 1:100
Sigma-Aldrich H3 antibody (Sigma-Aldrich, H9908) was used in immunohistochemistry - frozen section on mouse samples at 1:100. Biol Reprod (2013) ncbi
rat monoclonal (HTA28)
  • WB; human
Sigma-Aldrich H3 antibody (Sigma-Aldrich, H9908) was used in western blot on human samples. Nucleic Acids Res (2013) ncbi
Cell Signaling Technology
rabbit monoclonal (C36B11)
  • ChIP; human
Cell Signaling anti-H3K27me3 was used to perform ChIP assay in order to show that H3K27 methylation was regulated by PRC2 through dense chromatin.Science (2012) ncbi
  • WB; human
Cell Signaling anti-H3 was used to perform western blot in order to show that DNA repair could be promoted by SIRT6 through PARP1 activation under oxidative stress.Science (2011) ncbi
not indicated
  • WB; human
Cell Signaling anti-H3 was used to perform western blot in order to show that lysosomal biogenesis could be induced by TFEB and lead to autophagyScience (2011) ncbi
not indicated
  • WB; human
Cell Signaling H3 antibody was used in western blot to investigate the role of hippo signaling in the regulation of mammalian organ size.Proc Natl Acad Sci U S A (2010) ncbi
not indicated
  • WB; human
Cell Signaling total histone H3 antibody was used to perform western blot in order to investigate the relations between SGI-1776 and chronic lymphocytic leukemiaBlood (2009) ncbi
not indicated
  • ChIP; human
Cell signaling rabbit anti-H3Me2Lys27 was used to perform ChIP assays in order to illustrate that the Bmi1 functions on neurons oxidative metabolism are associated with repression of p53 pro-oxidant activity.J Neurosci (2009) ncbi
not indicated
  • WB; human
Cell Signaling anti-acetyl-histone H3 was used to perform western blot in order to study cytosine arabinoside could regulate EWS/FLI in ewing sarcoma by screening signature-based small molecule.PLoS Med (2007) ncbi
Articles Reviewed
  1. Simon Fischer et al. (2015). "miR-2861 as novel HDAC5 inhibitor in CHO cells enhances productivity while maintaining product quality".PMID 25997799
  2. Geetaram Sahu et al. (2015). "Cocaine promotes both initiation and elongation phase of HIV-1 transcription by activating NF-κB and MSK1 and inducing selective epigenetic modifications at HIV-1 LTR".PMID 25980739
  3. Kamalika Saha et al. (2015). "Methylosome Protein 50 and PKCδ/p38δ Protein Signaling Control Keratinocyte Proliferation via Opposing Effects on p21Cip1 Gene Expression".PMID 25851901
  4. Kee Beom Kim et al. (2015). "H3K9 methyltransferase G9a negatively regulates UHRF1 transcription during leukemia cell differentiation".PMID 25765655
  5. Elise Arbeille et al. (2015). "Cerebrospinal fluid-derived Semaphorin3B orients neuroepithelial cell divisions in the apicobasal axis".PMID 25721514
  6. Vinitha Jacob et al. (2015). "DNA hypomethylation induces a DNA replication-associated cell cycle arrest to block hepatic outgrowth in uhrf1 mutant zebrafish embryos".PMID 25564650
  7. Jiae Lee et al. (2015). "Coordinate control of terminal dendrite patterning and dynamics by the membrane protein Raw".PMID 25480915
  8. Darci M Fink et al. (2014). "Nerve growth factor regulates neurolymphatic remodeling during corneal inflammation and resolution".PMID 25383879
  9. Zhiqing Li et al. (2014). "Dynamics of polycomb proteins-mediated histone modifications during UV irradiation-induced DNA damage".PMID 25308962
  10. Azizollah Bakhtari et al. (2014). "The interfering effects of superovulation and vitrification upon some important epigenetic biomarkers in mouse blastocyst".PMID 25307438
  11. Petra van der Lelij et al. (2014). "SNW1 enables sister chromatid cohesion by mediating the splicing of sororin and APC2 pre-mRNAs".PMID 25257309
  12. Silvina V Sonzogni et al. (2015). "Nuclear translocation of p19INK4d in response to oxidative DNA damage promotes chromatin relaxation".PMID 25204969
  13. Kate Hawkins et al. (2014). "Novel cell lines isolated from mouse embryonic stem cells exhibiting de novo methylation of the E-cadherin promoter".PMID 25074424
  14. Tao Lu et al. (2014). "REST and stress resistance in ageing and Alzheimer's disease".PMID 24670762
  15. Xiaochang Zhang et al. (2014). "Mutations in QARS, encoding glutaminyl-tRNA synthetase, cause progressive microcephaly, cerebral-cerebellar atrophy, and intractable seizures".PMID 24656866
  16. Konstantin Glebov et al. (2014). "Mechanisms underlying dual effects of serotonin during development of Helisoma trivolvis (Mollusca)".PMID 24625099
  17. Nataki C Douglas et al. (2013). "Investigating the role of tbx4 in the female germline in mice".PMID 24089201
  18. Priscilla Nga Ieng Lau et al. (2013). "Elucidating combinatorial histone modifications and crosstalks by coupling histone-modifying enzyme with biotin ligase activity".PMID 23258705
  19. Hennady P Shulha et al. (2012). "Human-specific histone methylation signatures at transcription start sites in prefrontal neurons".PMID 23185133
  20. Wen Yuan et al. (2012). "Dense chromatin activates Polycomb repressive complex 2 to regulate H3 lysine 27 methylation".PMID 22923582
  21. Jia Fei et al. (2011). "Regulation of nucleotide excision repair by UV-DDB: prioritization of damage recognition to internucleosomal DNA".PMID 22039351
  22. Luciano DiTacchio et al. (2011). "Histone lysine demethylase JARID1a activates CLOCK-BMAL1 and influences the circadian clock".PMID 21960634
  23. Zhiyong Mao et al. (2011). "SIRT6 promotes DNA repair under stress by activating PARP1".PMID 21680843
  24. Carmine Settembre et al. (2011). "TFEB links autophagy to lysosomal biogenesis".PMID 21617040
  25. Sean C Bendall et al. (2011). "Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum".PMID 21551058
  26. Karen T Smith et al. (2010). "Deacetylase inhibitors dissociate the histone-targeting ING2 subunit from the Sin3 complex".PMID 20142042
  27. Li Lu et al. (2010). "Hippo signaling is a potent in vivo growth and tumor suppressor pathway in the mammalian liver".PMID 20080689
  28. Lisa S Chen et al. (2009). "Pim kinase inhibitor, SGI-1776, induces apoptosis in chronic lymphocytic leukemia cells".PMID 19734450
  29. Jorrit V Tjeertes et al. (2009). "Screen for DNA-damage-responsive histone modifications identifies H3K9Ac and H3K56Ac in human cells".PMID 19407812
  30. Lanjun Xu et al. (2009). "PDCD5 interacts with Tip60 and functions as a cooperator in acetyltransferase activity and DNA damage-induced apoptosis".PMID 19308289
  31. Lin Feng et al. (2009). "MERIT40 facilitates BRCA1 localization and DNA damage repair".PMID 19261748
  32. Daniel Lucas et al. (2009). "Altered hematopoiesis in mice lacking DNA polymerase mu is due to inefficient double-strand break repair".PMID 19229323
  33. Fabienne T Schulthess et al. (2009). "Deletion of the mitochondrial flavoprotein apoptosis inducing factor (AIF) induces beta-cell apoptosis and impairs beta-cell mass".PMID 19197367
  34. Cristina A de Frutos et al. (2009). "Snail1 controls bone mass by regulating Runx2 and VDR expression during osteoblast differentiation".PMID 19197242
  35. Wassim Chatoo et al. (2009). "The polycomb group gene Bmi1 regulates antioxidant defenses in neurons by repressing p53 pro-oxidant activity".PMID 19144853
  36. Moon Kyoo Jang et al. (2009). "Papillomavirus E2 proteins and the host BRD4 protein associate with transcriptionally active cellular chromatin".PMID 19129460
  37. Susan Nozell et al. (2008). "The ING4 tumor suppressor attenuates NF-kappaB activity at the promoters of target genes".PMID 18779315
  38. Adrianne E Hontz et al. (2008). "Expression of selected Aurora A kinase substrates in solely estrogen-induced ectopic uterine stem cell tumors in the Syrian hamster kidney".PMID 18497064
  39. Tõnis Org et al. (2008). "The autoimmune regulator PHD finger binds to non-methylated histone H3K4 to activate gene expression".PMID 18292755
  40. Kimberly Stegmaier et al. (2007). "Signature-based small molecule screening identifies cytosine arabinoside as an EWS/FLI modulator in Ewing sarcoma".PMID 17425403
  41. Aymeric Ravel-Chapuis et al. (2007). "Postsynaptic chromatin is under neural control at the neuromuscular junction".PMID 17304221
  42. Ying Yang et al. (2005). "Tissue-specific regulation of the mouse alphaA-crystallin gene in lens via recruitment of Pax6 and c-Maf to its promoter".PMID 16023139