This is a review about H2AX, based on 54 published articles (read how Labome selects the articles), using H2AX antibody in experiments. It is aimed to help Labome visitors find the most suited H2AX antibody.
H2AX synonym: H2A.X; H2A/X; H2AX; H2AX histone; histone H2A.x; histone H2AX

  • Upstate anti-gamma-H2AX was used to perform immunocytochemistry in order to show that RHINO is important for ATR activation during DNA damage responses. (catalog: 05-636) ncbi, more , figure 1
  • Figure 1
    H2AX antibody immunocytochemistry EMD Millipore
    H2AX antibody immunocytochemistry EMD Millipore
    Hus1-dependent RHINO recruitment to sites of DSBs. MEFs (WT, RAD18-/-, HUS1-/-, and HUS1-/- reconstituted with HUS1) expressing GFP-RHINO had DSBs introduced by UV laser treatment, and RHINO recruitment was assessed.
    Cecilia Cotta-Ramusino et al. (2011). "A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1 interacting protein required for ATR signaling".PMID 21659603
  • Millipore Gamma-H2AX antibody was used to perform western blot assay in order to study the requirement of RSFI in DSB repair.ncbi, more , figure 2
  • Figure 2
    H2AX antibody western blot EMD Millipore
    H2AX antibody western blot EMD Millipore
    RSF1 is required for the recruitment of CENPS/MHF1, CENPX/MHF2, FANCD2, and FANCI to IRIF. (A-F) Immunofluorescence of the indicated proteins 60 min after IR (4 Gy) in the indicated siRNA-treated U2OS cells: (A) p-ATM and 53BP1, (B) MHF1 and Gamma-H2AX,
    Fabio Pessina et al. (2014). "The RSF1 histone-remodelling factor facilitates DNA double-strand break repair by recruiting centromeric and Fanconi Anaemia proteins".PMID 24800743
  • Upstate rabbit anti-phosph-H2AX antibody was used to carry out western blot analysis and immunofluorescence assays in order to clarify the mechanism for SIRT6 promoting DNA end resection. (catalog: 05-636) ncbi, more
  • Upstate anti-gammaH2AX was used to perform immunocytochemistry in order to show that chromosomal structural aberrations could be caused by chromosome segregation errors. (catalog: 05-636) ncbi, more
  • Upstate anti-H2AFX antibody 1:1,000 was used for western blot in order to investigate the roles of H3K9Ac and H3K56Ac in the regulation of DNA-damage-responsive in human cells (catalog: 05-636) ncbi, more
  • Upstate anti-phospho-H2AX antibody was used to carry out western blot analysis in order to determine the histone H3 lysine 56 acetylation by histone acetyl transferases CBP/p300. (catalog: 07-164) ncbi, more
  • Upstate rabbit polyclonal anti-phosph-H2AX antibody was used to carry out western blot analysis in order to investigate the role of DNA polymerase mu in physiological hematopoietic development. (catalog: 07-164) ncbi, more
  • Upstate anti-H2AX and anti-H2AX antibodies were used in western blot in order to study the role of TRAIL for activation of DNA damage response pathways with histone H2AX and Chk2 phosphorylations. (catalog: 05-636,07-627) ncbi, more
  • suppliernumreagent linkcited catalog
    EMD Millipore23H2AX antibody05-636, 07-164, 05-636,07-627
    Abcam3H2AX antibodyab8108
    R and D Systems1H2AX antibody
    Novus Biologicals1H2AX antibody
    Cell Signaling Technology4H2AX antibody
    Trevigen1
    New England Biolabs1
    Table 1. H2AX antibody western blot.
  • Upstate Biotechnology anti-phospho-H2AX antibody (catalog no. 07-164) was used in western blot to study the function of Frag1 protein in higher eukaryotes. (catalog: 07-164) ncbi, more
  • Upstate Biotechnology mouse monoclonal anti-phospho-H2AX (catalog number 05-636) antibody was used in western blot to study the benzo[a]pyrene-dihydrodiol epoxide (BPDE) induced S-phase checkpoint (catalog: 05-636) ncbi, more
  • Millipore mouse anti-gammaH2AX antibody was used to perform western blot in order to study the mechanism through which doxorubicin suppressed tumor growth.ncbi, more
  • Upstate Biotechnology gamma-H2AX mouse antibody was used in immunohistochemistry in order to study the effect of the DNA damage on HCC development and recurrence.ncbi, more
  • Upstate gammaH2AX antibody was used in immunohistochemistry in order to show the importance of protection of the ATM-mediated DDR-p53 signaling pathway for breast cancer prevention.ncbi, more
  • suppliernumreagent linkcited catalog
    EMD Millipore19H2AX antibody05-636
    Abcam3H2AX antibodyab22551, ab8108
    Novus Biologicals1H2AX antibody
    BioLegend1H2AX antibody
    Cell Signaling Technology3H2AX antibody
    Sigma-Aldrich1
    New England Biolabs1
    Table 2. H2AX antibody immunocytochemistry.
  • Millipore mouse FITC anti-gamma-H2AX antibody was used in western blot to investigate the role of RNF8 and RNF168 in the regulation of class switch recombination.ncbi, more
  • Upstate Biotechnology gamma-H2AX antibody was used in western blot to investigate the role of RNF8 and RNF168 in the regulation of class switch recombination.ncbi, more
  • Upstate anti-phospho-Histone H2AX antibody was used to perform western blot in order to study the interaction between F1L and endogenous Bak.ncbi, more
  • Upstate anti--phopho-H2AX antibody was used to carry out immunofluorescence assays in order to investigate the effect of adeno-associated virus and adenovirus coinfection on inducing cellular DNA damage response.ncbi, more
  • Upstate Biotechnologies anti-gamma H2AX antibody was used in immunocytochemistry to study the role for NBA1 in IR resistance, cell cycle regulation, and the recruitment of BRCA1 to sites of DNA damage.ncbi, more
  • suppliernumreagent link
    EMD Millipore3H2AX antibody
    Table 3. H2AX antibody immunohistochemistry.
  • Upstate Biotechnologies mouse monoclonal anti-gammaH2AX antibody (clone JBW301) was used in immunocytochemistry to study the role of MERIT40 in controlling BRCA1 Rap80 complex integrity and recruitment to DNA double-strand breaks.ncbi, more
  • Upstate Biotechnology rabbit anti-H2AX antibody was used for western blot in order to investigate the role of Syce1 in the regulation of Synapsis and DNA Repair.ncbi, more
  • Upstate Biotechnology mouse monoclonal anti-H2AX antibody was used for western blot in order to investigate the role of Aurora B in the regulation of postmitotic endoreduplication checkpoint.ncbi, more
  • Upstate anti-gamma-H2AX antibody was used to perform immunohistochemistry in order to show that the p53 signature and endometrial glandular dysplasia often present at the beginning of serous intraepithelial carcinoma.ncbi, more
  • Upstate anti-phospho-histone H2A.X monoclonal antibody was used to perform western blot in order to illustrate that the Bmi1 functions on neurons oxidative metabolism are associated with repression of p53 pro-oxidant activity.ncbi, more
  • suppliernumreagent link
    EMD Millipore1H2AX antibody
    Abcam1H2AX antibody
    Table 4. H2AX antibody ChIP assay.
  • Upstate Biotechnology mouse monoclonal anti-phospho-histone H2AX antibody was used in flow cytometry to study the role of TLS DNA polymerases eta and kappa in the regulation in human cancer cells.ncbi, more
  • Upstate anti-gammaH2AX antibody was used in immunocytochemistry in order to determine the effect of HMGN1 on ATM activation by regulating the levels of histone modifications.ncbi, more
  • Upstate anti-phosphorylated histone H2AX antibody was used in immunocytochemistry in order to study the role for FA pathways in the maintenance of differentiation-dependent cell cycle exitncbi, more
  • Millipore monoclonal anti-gamma-H2AX antibody (JBW301) was used in western blot to study the effect of simian virus 40 large T antigen on genome integrity and DNA damage response.ncbi, more
  • Upstate H2AX p139S antibody was used in immunocytochemistry in order to identify ataxia-telangiectasia mutated (ATM) as a further component of the complex signalling network of radiation-induced DNA damage in non-targeted bystander cells downstream of ataxia-telangiectasia and Rad3-related (ATR)ncbi, more
  • suppliernumreagent link
    EMD Millipore1H2AX antibody
    Table 5. H2AX antibody immunoprecipitation.
  • Upstate Biotechnology mouse monoclonal anti-gamma-H2AX antibody was used in immunocytochemistry to study the role for DEAD Box 1 in the repair of transcriptionally active regions of the genome.ncbi, more
  • Upstate Biotechnology anti-pS139 H2AX antibody was used to carry out western blot analysis in order to investigate the role for WRN in ATM pathway activation and the S-phase checkpoint in cells exposed to DNA interstrand cross-link-induced double-strand breaks.ncbi, more
  • Upstate monoclonal H2AX antibody was used in western blot in order to demonstrate that ATM undergoes an activating phosphorylation in syncytia elicited by the envelope glycoprotein complex (Env) of human immunodeficiency virus-1 (HIV-1) in vitroncbi, more
  • Calbiochem anti-gamma-H2AX antibody was used in immunocytochemistry to show the synergistic effect of VPA and cisplatin on ovarian cancer cells.ncbi, more
  • Up-state Biotechnology anti-gamma-H2AX antibody was used to perform immunocytochemistry in order to show that BRIT1 can regulate DNA damage as a tumor suppressor.ncbi, more
  • suppliernumreagent link
    EMD Millipore1H2AX antibody
    Table 6. H2AX antibody flow cytometry.
  • Upstate 1:200 diluted antiphospho-histone H2AX (Ser-139) antibody was used in immunocytochemistry to reveal the status of histone H3 and H2AX phosphorylation on Ser-10 and Ser-139 as well as ATM activation through phosphorylation on Ser-1981 during PCC.ncbi, more
  • Upstate cell signaling solutions antibody against gamma-H2AX was used in western blot and immunocytochemistry to detect gamma-H2AX in human fibroblast cell line DR95.ncbi, more
  • Upstate Biotechnology monoclonal anti-gammaH2AX antibody was used in western blot to demonstrate that BRIT1 acts as a regulator of both the intra-S and G2/M checkpoints and the checkpoint defects in the absence of BRIT1 are likely to result from its regulation of Nbs1, BRCA1, and Chk1.ncbi, more
  • Upstate Biotechnology antibody against phospho-H2AX was used in western blot to detect H2AX phosphorylation in human HCT116 and 293T cellsncbi, more
  • Upstate anti-phosphohistone H2A.X antibody (S139) antibody was used in immunocytochemistry to identify ATM domains for nuclear localization and chromatin associationncbi, more
  • Upstate Cell Signaling Solutions anti-phospho-H2AX monoclonal antibodies were used in immunocytochemistry to detect the localization of phosphorylated (gamma) H2AX in human HeLa cells, MEF (mouse embryonic fibroblast) cells, and mouse NIH3T3 cellsncbi, more
  • Upstate H2AX-S139 antibody was used in western blot to study the effect of Epstein-Barr virus lytic replication on ATM checkpoint signal transductionncbi, more
  • Cell Signaling anti-gamma-H2AX antibody was used in immunocytochemistry to study the regulation of human Rif1, ortholog of a yeast telomeric protein by ATM and 53BP1 and functions in the S-phase checkpoint.ncbi, more
  • Upstate Biotechnology rabbit anti-gamma-H2AX and mouse anti-gamma-H2AX (clone S139) antibodies were used in immunoprecipitation, immunocytochemistry, and ChIP assay to study the replicative senescence triggered by DNA damage checkpoint kinase Chk2.ncbi, more
  • Upstate Cell Signaling Solutions anti-gammaH2AX was used in immunocytochemistry to stain for gammaH2AX localization in HeLa cells.ncbi, more
  • Upstate Biotech anti-gammaH2AX polyclonal antibody was used in western blot to study the DNA damage response and MCL-1 destruction initiating apoptosis in adenovirus-infected cells.ncbi, more
  • Abcam rabbit anti-CHK2 antibody was used to carry out western blot analysis and immunofluorescence assays in order to clarify the mechanism for SIRT6 promoting DNA end resection. (catalog: ab8108) ncbi, more
  • Abcam rabbit anti-H2AX antibody was used to carry out western blot analysis in order to clarify the mechanism for SIRT6 promoting DNA end resection.ncbi, more
  • Abcam anti-gammaH2Ax was used to perform immunocytochemistry in order to show that DNA repair could be promoted by SIRT6 through PARP1 activation under oxidative stress. (catalog: ab22551) ncbi, more
  • Abcam anti-H2AX antibody 1:1,000 was used for western blot in order to investigate the roles of H3K9Ac and H3K56Ac in the regulation of DNA-damage-responsive in human cellsncbi, more
  • Abcam anti-H2A-pS121 antibody was used to carry out ChIP assay and immunostaining assays in order to investigate the mechanism for how inner centromere is established and how the CPC is targeted to this site.ncbi, more
  • R&D Systems H2AX histone antibody was used in western blot in order to identify host genes specifically targeted by K12 by comparing their responses with responses elicited by pathogensncbi, more
  • Novus Biologicals anti-phosph H2AX antibody was used to carry out immunofluorescence assays in order to investigate the role of EBNA-1 in inducing chromosomal aberrations by production of reactive oxygen species.ncbi, more
  • Novus anti-H2AX antibody was used to carry out western blot analysis in order to investigate the role for WRN in ATM pathway activation and the S-phase checkpoint in cells exposed to DNA interstrand cross-link-induced double-strand breaks.ncbi, more
  • BioLegend mouse monoclonal anti-phospho-specific (Ser-139) histone H2AX (gammaH2AX) was used in 1:200 dilution to perform immunocytochemistry in order to show that in A549 cells the molecular machinery such as the recruitment of Mre11 and phosphorylation of ATM, Chk2, and H2AX (gammaH2AX) induced under oxidative stress.ncbi, more
  • Cell Signaling Technology rabbit anti-phosph-H2AX antibody was used to carry out western blot analysis and immunofluorescence assays in order to clarify the mechanism for SIRT6 promoting DNA end resection.ncbi, more
  • Cell Signaling anti-gammaH2AX was used to perform immunocytochemistry in order to show that chromosomal structural aberrations could be caused by chromosome segregation errors.ncbi, more
  • Cell Signalling Technology monoclonal anti-gammaH2AX was used to perform western blot and immunocytochemistry in order to show that cell abscission mediated by Aurora B was regulated by ESCRT-III.ncbi, more
  • Cell Signaling phospho-H2ax antibody (Ser139) was used to perform western blot in order to clarify that Skp2 involved in the p19Arf-p53 pathway independent cellular senescence.ncbi, more
  • Cell Signaling anti-H2AFX antibody was used for western blot in order to investigate the role of Chk2 in the regulation of HSV-1 ICP0-mediated G2/M arrest and virus growthncbi, more
Sigma-Aldrich
  • Sigma anti-Gamma H2AX monoclonal antibody was used to carry out immunofluorescence assays in order to investigate the role for aprataxin in mouse primary neural cells lacking Tdp1 during chromosomal single strand break repair.ncbi, more
LP BIO
  • LP Bio anti-gamma-H2AX antibody was used in western blot in order to investigate the mechanism of how the embryonic stem cells chromatin structure change was regulated by condensin complexes during mitotic progression.ncbi, more
New England Biolabs
  • NEB polyclonal anti-gammaH2AX was used to perform western blot and immunocytochemistry in order to show that cell abscission mediated by Aurora B was regulated by ESCRT-III.ncbi, more
Trevigen
  • Trevigen gamma-H2AX antibody was used in western blot to study p53 mitochondria translocationncbi, more
Articles Reviewed
  1. Fabio Pessina et al. (2014). "The RSF1 histone-remodelling factor facilitates DNA double-strand break repair by recruiting centromeric and Fanconi Anaemia proteins".PMID 24800743
  2. Bray Denard et al. (2012). "Doxorubicin blocks proliferation of cancer cells through proteolytic activation of CREB3L1".PMID 23256041
  3. Jeremy G Carlton et al. (2012). "ESCRT-III governs the Aurora B-mediated abscission checkpoint through CHMP4C".PMID 22422861
  4. Aniek Janssen et al. (2011). "Chromosome segregation errors as a cause of DNA damage and structural chromosome aberrations".PMID 21960636
  5. Zhiyong Mao et al. (2011). "SIRT6 promotes DNA repair under stress by activating PARP1".PMID 21680843
  6. Cecilia Cotta-Ramusino et al. (2011). "A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1 interacting protein required for ATR signaling".PMID 21659603
  7. Yuya Yamagishi et al. (2010). "Two histone marks establish the inner centromere and chromosome bi-orientation".PMID 20929775
  8. Abderrahmane Kaidi et al. (2010). "Human SIRT6 promotes DNA end resection through CtIP deacetylation".PMID 20829486
  9. Hui Kuan Lin et al. (2010). "Skp2 targeting suppresses tumorigenesis by Arf-p53-independent cellular senescence".PMID 20237562
  10. Thomas G Fazzio et al. (2010). "Condensin complexes regulate mitotic progression and interphase chromatin structure in embryonic stem cells".PMID 20176923
  11. Hila Barash et al. (2010). "Accelerated carcinogenesis following liver regeneration is associated with chronic inflammation-induced double-strand DNA breaks".PMID 20133864
  12. Jay P Reddy et al. (2010). "Defining the ATM-mediated barrier to tumorigenesis in somatic mammary cells following ErbB2 activation".PMID 20133707
  13. Shaliny Ramachandran et al. (2010). "The RNF8/RNF168 ubiquitin ligase cascade facilitates class switch recombination".PMID 20080757
  14. Stephanie Campbell et al. (2010). "Vaccinia virus F1L interacts with Bak using highly divergent Bcl-2 homology domains and replaces the function of Mcl-1".PMID 19955184
  15. Jorrit V Tjeertes et al. (2009). "Screen for DNA-damage-responsive histone modifications identifies H3K9Ac and H3K56Ac in human cells".PMID 19407812
  16. Rachel A Schwartz et al. (2009). "Adeno-associated virus replication induces a DNA damage response coordinated by DNA-dependent protein kinase".PMID 19339345
  17. Sherif F El-Khamisy et al. (2009). "Synergistic decrease of DNA single-strand break repair rates in mouse neural cells lacking both Tdp1 and aprataxin".PMID 19303373
  18. Chandrima Das et al. (2009). "CBP/p300-mediated acetylation of histone H3 on lysine 56".PMID 19270680
  19. Bin Wang et al. (2009). "NBA1, a new player in the Brca1 A complex, is required for DNA damage resistance and checkpoint control".PMID 19261749
  20. Genze Shao et al. (2009). "MERIT40 controls BRCA1-Rap80 complex integrity and recruitment to DNA double-strand breaks".PMID 19261746
  21. Ewelina Bolcun-Filas et al. (2009). "Mutation of the mouse Syce1 gene disrupts synapsis and suggests a link between synaptonemal complex structural components and DNA repair".PMID 19247432
  22. Daniel Lucas et al. (2009). "Altered hematopoiesis in mice lacking DNA polymerase mu is due to inefficient double-strand break repair".PMID 19229323
  23. Jayasree S Nair et al. (2009). "Aurora B kinase regulates the postmitotic endoreduplication checkpoint via phosphorylation of the retinoblastoma protein at serine 780".PMID 19225156
  24. Elke A Jarboe et al. (2009). "Evidence for a latent precursor (p53 signature) that may precede serous endometrial intraepithelial carcinoma".PMID 19151662
  25. Wassim Chatoo et al. (2009). "The polycomb group gene Bmi1 regulates antioxidant defenses in neurons by repressing p53 pro-oxidant activity".PMID 19144853
  26. Bettina Gruhne et al. (2009). "The Epstein-Barr virus nuclear antigen-1 promotes genomic instability via induction of reactive oxygen species".PMID 19139406
  27. Remy Betous et al. (2009). "Role of TLS DNA polymerases eta and kappa in processing naturally occurring structured DNA in human cells".PMID 19117014
  28. Yong Chul Kim et al. (2009). "Activation of ATM depends on chromatin interactions occurring before induction of DNA damage".PMID 19079244
  29. E E Hoskins et al. (2009). "Fanconi anemia deficiency stimulates HPV-associated hyperplastic growth in organotypic epithelial raft culture".PMID 19015634
  30. Stéphanie Solier et al. (2009). "Death receptor-induced activation of the Chk2- and histone H2AX-associated DNA damage response pathways".PMID 18955500
  31. Jennifer Hein et al. (2009). "Simian virus 40 large T antigen disrupts genome integrity and activates a DNA damage response via Bub1 binding".PMID 18922873
  32. Susanne Burdak-Rothkamm et al. (2008). "ATM acts downstream of ATR in the DNA damage response signaling of bystander cells".PMID 18757420
  33. Lei Li et al. (2008). "A role for DEAD box 1 at DNA double-strand breaks".PMID 18710941
  34. Celine Cosseau et al. (2008). "The commensal Streptococcus salivarius K12 downregulates the innate immune responses of human epithelial cells and promotes host-microbe homeostasis".PMID 18625732
  35. Wen Hsing Cheng et al. (2008). "WRN is required for ATM activation and the S-phase checkpoint in response to interstrand cross-link-induced DNA double-strand breaks".PMID 18596239
  36. Jean Luc Perfettini et al. (2008). "Critical involvement of the ATM-dependent DNA damage response in the apoptotic demise of HIV-1-elicited syncytia".PMID 18560558
  37. Ching Tai Lin et al. (2008). "Valproic acid resensitizes cisplatin-resistant ovarian cancer cells".PMID 18429963
  38. Hong Zhao et al. (2008). "Oxidative stress induces cell cycle-dependent Mre11 recruitment, ATM and Chk2 activation and histone H2AX phosphorylation".PMID 18418078
  39. Han Li et al. (2008). "Chk2 is required for HSV-1 ICP0-mediated G2/M arrest and enhancement of virus growth".PMID 18321553
  40. Rekha Rai et al. (2006). "BRIT1 regulates early DNA damage response, chromosomal integrity, and cancer".PMID 16872911
  41. Xuan Huang et al. (2006). "Sequential phosphorylation of Ser-10 on histone H3 and ser-139 on histone H2AX and ATM activation during premature chromosome condensation: relationship to cell-cycle phase and apoptosis".PMID 16528736
  42. Amélie Rodrigue et al. (2006). "Interplay between human DNA repair proteins at a unique double-strand break in vivo".PMID 16395335
  43. Shiaw Yih Lin et al. (2005). "BRIT1/MCPH1 is a DNA damage responsive protein that regulates the Brca1-Chk1 pathway, implicating checkpoint dysfunction in microcephaly".PMID 16217032
  44. Hui Zhong et al. (2005). "Rad50 depletion impacts upon ATR-dependent DNA damage responses".PMID 16087684
  45. Hideshi Ishii et al. (2005). "Frag1, a homolog of alternative replication factor C subunits, links replication stress surveillance with apoptosis".PMID 15983387
  46. David B Young et al. (2005). "Identification of domains of ataxia-telangiectasia mutated required for nuclear localization and chromatin association".PMID 15929992
  47. Fiona Pryde et al. (2005). "53BP1 exchanges slowly at the sites of DNA damage and appears to require RNA for its association with chromatin".PMID 15840649
  48. Xiaohui Bi et al. (2005). "DNA polymerase kappa is specifically required for recovery from the benzo[a]pyrene-dihydrodiol epoxide (BPDE)-induced S-phase checkpoint".PMID 15817457
  49. Yoshimi Arima et al. (2005). "Transcriptional blockade induces p53-dependent apoptosis associated with translocation of p53 to mitochondria".PMID 15753095
  50. Ayumi Kudoh et al. (2005). "Epstein-Barr virus lytic replication elicits ATM checkpoint signal transduction while providing an S-phase-like cellular environment".PMID 15611093
  51. Joshua Silverman et al. (2004). "Human Rif1, ortholog of a yeast telomeric protein, is regulated by ATM and 53BP1 and functions in the S-phase checkpoint".PMID 15342490
  52. Véronique Gire et al. (2004). "DNA damage checkpoint kinase Chk2 triggers replicative senescence".PMID 15192702
  53. Jacob G Robison et al. (2004). "Replication protein A and the Mre11.Rad50.Nbs1 complex co-localize and interact at sites of stalled replication forks".PMID 15180989
  54. Andrea Cuconati et al. (2003). "DNA damage response and MCL-1 destruction initiate apoptosis in adenovirus-infected cells".PMID 14633975