This is a review about GJB2, based on 38 published articles (read how Labome selects the articles), using GJB2 antibody in all methods. It is aimed to help Labome visitors find the most suited GJB2 antibody.
GJB2 synonym: CX26; DFNA3; DFNA3A; DFNB1; DFNB1A; HID; KID; NSRD1; PPK; connexin 26; gap junction protein, beta 2, 26kDa; mutant gap junction protein beta 2

Invitrogen
rabbit polyclonal
  • IC; human
In order to study the synthesis and localization of connexin 30, Invitrogen GJB2 antibody (Invitrogen, 51-2800) was used in immunocytochemistry on human samples. J Cell Sci (2015) ncbi
rabbit polyclonal
  • IHC; mouse; 1:200
In order to study vestibular dysfunction and organ pathology in connexin-26 knock out mice, Invitrogen GJB2 antibody (Invitrogen, 51-2800) was used in immunohistochemistry on mouse samples at 1:200. Hear Res (2015) ncbi
rabbit polyclonal
  • IHC-P; mouse; 1 ug/ml
  • WB; mouse; 1:1000
In order to test if K5-Cx26 mothers fail to eject the milk, Invitrogen GJB2 antibody (Invitrogen, Cat#51-2800) was used in immunohistochemistry - paraffin section on mouse samples at 1 ug/ml and in western blot on mouse samples at 1:1000. Dev Biol (2015) ncbi
rabbit polyclonal
  • IC; mouse; 1:200
In order to show that Gjb2 is required for normal Corti development in mice, Invitrogen GJB2 antibody (Invitrogen, 51-2800) was used in immunocytochemistry on mouse samples at 1:200. Neurobiol Dis (2015) ncbi
mouse monoclonal (CX-12H10)
  • IHC; rat; 1:100; fig 2
In order to examine the relationship between Cx26 and presbycusis, Invitrogen GJB2 antibody (Invitrogen, 138100) was used in immunohistochemistry on rat samples at 1:100 (fig 2). Biochem Biophys Res Commun (2014) ncbi
rabbit polyclonal
  • IC; mouse; fig 1
In order to determine if patients with loss-of-function Cx26 mutations have a greater susceptibility to impaired breast development, Invitrogen GJB2 antibody (Invitrogen, 51-2800) was used in immunocytochemistry on mouse samples (fig 1). PLoS ONE (2014) ncbi
mouse monoclonal (CX-12H10)
  • WB; mouse; 1:500
  • IHC; mouse; 1:500
In order to investigate the effect of the Clouston syndrome mutation connexin30 A88V on the proliferation of sebaceous glands and hearing in mice, Invitrogen GJB2 antibody (Invitrogen, 13-8100) was used in western blot on mouse samples at 1:500 and in immunohistochemistry on mouse samples at 1:500. FEBS Lett (2014) ncbi
mouse monoclonal (CX-12H10)
  • IHC-F; human; 1:100
In order to investigate the expression of gap junction proteins in Dupuytren disease, Invitrogen GJB2 antibody (Invitrogen, 13-8100) was used in immunohistochemistry - frozen section on human samples at 1:100. Acta Orthop (2014) ncbi
rabbit polyclonal
  • IHC; mouse; 1:200
In order to characterize mice that lack connexin 26 in the non-sensory cells flanking the auditory epithelium, Invitrogen GJB2 antibody (Invitrogen, 51-2800) was used in immunohistochemistry on mouse samples at 1:200. Hear Res (2014) ncbi
rabbit polyclonal
  • IC; mouse
  • IC; rat
In order to characterize connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus, Invitrogen GJB2 antibody (Life Technologies, 51-2800) was used in immunocytochemistry on mouse samples and on rat samples. Eur J Neurosci (2014) ncbi
rabbit polyclonal
  • IC; mouse; 1:200
In order to study the role of connexins in melanoma tumorigenesis and assess their status during cancer onset and progression, Invitrogen GJB2 antibody (Invitrogen, 51-2800) was used in immunocytochemistry on mouse samples at 1:200. J Biol Chem (2014) ncbi
mouse monoclonal (CX-12H10)
  • IHC-P; human; 1:250
  • WB; human; 1:500
In order to investigate the expression of connexin 43 in eutopic endometrium and cultured endometrial stromal cells, Invitrogen GJB2 antibody (Life Technologies, 13-8100) was used in immunohistochemistry - paraffin section on human samples at 1:250 and in western blot on human samples at 1:500. Mol Hum Reprod (2014) ncbi
mouse monoclonal (CX-12H10)
  • WB; mouse; fig 1
In order to study the role of improved intercellular gap junction communication in the mechanism by which tanshinone IIA enhances the bystander effect during HSV-thymidine kinase/ganciclovire gene therapy, Invitrogen GJB2 antibody (Zymed, CX-12H10) was used in western blot on mouse samples (fig 1). PLoS ONE (2013) ncbi
rabbit polyclonal
  • WB; mouse; 1:500; fig 4
In order to assess the effect of the +/G60S mutation in connexin 43 following stroke, Invitrogen GJB2 antibody (Zymed, 51-2800) was used in western blot on mouse samples at 1:500 (fig 4). Neuropharmacology (2013) ncbi
mouse monoclonal (CX-12H10)
  • IHC-P; mouse; fig 1
  • WB; human; fig 2
  • IC; human; fig 2
In order to elucidate the role of p63 in deafness, Invitrogen GJB2 antibody (Invitrogen, 13-8100) was used in immunohistochemistry - paraffin section on mouse samples (fig 1), in western blot on human samples (fig 2), and in immunocytochemistry on human samples (fig 2). Proc Natl Acad Sci U S A (2013) ncbi
rabbit polyclonal
  • IHC-F; mouse; 3 ug/ml; fig 2
In order to study localization of connexins in the brain, Invitrogen GJB2 antibody (Invitrogen, 51-2800) was used in immunohistochemistry - frozen section on mouse samples at 3 ug/ml (fig 2). Eur J Neurosci (2011) ncbi
rabbit polyclonal
  • IHC; mouse; fig 5
In order to study the expression and function of connexin26 and connexin30 in the ears of transgenic mice, Invitrogen GJB2 antibody (Invitrogen, 51-2800) was used in immunohistochemistry on mouse samples (fig 5). PLoS ONE (2011) ncbi
rabbit polyclonal
  • IHC-F; mouse; 1:25; fig 7
In order to examine the function of focal adhesion kinase in cortical interneurons and pyramidal cells, Invitrogen GJB2 antibody (Zymed, 51-2800) was used in immunohistochemistry - frozen section on mouse samples at 1:25 (fig 7). J Neurosci (2011) ncbi
mouse monoclonal (CX-12H10)
  • IC; mouse; 1:500; fig 3
In order to study connexin 26 and connexin 32 isoforms in the cochlea, Invitrogen GJB2 antibody (Invitrogen, 13-8100) was used in immunocytochemistry on mouse samples at 1:500 (fig 3). Eur J Cell Biol (2011) ncbi
rabbit polyclonal
  • WB; mouse; fig 1
In order to study connexin 26 in astrocytes, Invitrogen GJB2 antibody (Invitrogen, 51-2800) was used in western blot on mouse samples (fig 1). Eur J Neurosci (2011) ncbi
rabbit polyclonal
  • WB; rat; 1:500; tbl 1
In order to investigate the role of connexins in the production of reactive oxygen-nitrogen species and astrocytic gap junctional communication in diabetic rats, Invitrogen GJB2 antibody (Invitrogen, 51-2800) was used in western blot on rat samples at 1:500 (tbl 1). J Neurosci Res (2011) ncbi
not indicated
  • IP; human
Zymed Laboratories anti-Cxs26 was used as primary antibody in immunoprecipitation in order to prove that the mutations at the two contributor genes of non-syndromic deafness can cause hearing loss in a heterozygous condition.Hum Genet (2009) ncbi
rabbit polyclonal
  • IHC; mouse; tbl 1
In order to study the extent of coupling in microvessels of the rodent brainstem and retina and the subtypes of intracellular calcium stores that mediate astrocytic signaling, Invitrogen GJB2 antibody (Zymed, 51-2800) was used in immunohistochemistry on mouse samples (tbl 1). J Comp Neurol (2008) ncbi
rabbit polyclonal
  • WB; mouse; fig 1
In order to study of the spatial and temporal pattern of connexin expression in the developing brain, Invitrogen GJB2 antibody (Zymed, 51-2800) was used in western blot on mouse samples (fig 1). J Comp Neurol (2007) ncbi
not indicated
  • IC; human
Zymed Laboratories Cx26 antibody (1:200) was used in immunocytochemistry to demonstrate that up-regulation of Cx26 or slowing down its protein degradation might be a therapeutic strategy to prevent and treat deafness caused by Cx30 mutations.Proc Natl Acad Sci U S A (2007) ncbi
rabbit polyclonal
  • IHC-F; mouse; 1:200
In order to examine the connexin associations at oligodendrocyte/astrocyte gap junctions on oligodendrocytes in normal and Cx32 knockout mice, Invitrogen GJB2 antibody (Zymed, 51?C2800) was used in immunohistochemistry - frozen section on mouse samples at 1:200. Glia (2003) ncbi
mouse monoclonal (CX-12H10)
  • IC; human; fig 3
In order to examine the relationships among HPV infection, gap junctions, and tumor progression, Invitrogen GJB2 antibody (Zymed, 13-8100) was used in immunocytochemistry on human samples (fig 3). Oncogene (2003) ncbi
mouse monoclonal (CX-12H10)
  • IC; human; fig 4
In order to study connexins and gap junction proteins in endometrial carcinoma cells, Invitrogen GJB2 antibody (Zymed, CX-12H10) was used in immunocytochemistry on human samples (fig 4). Carcinogenesis (2003) ncbi
mouse monoclonal (CX-12H10)
  • IHC; rat; 10 ug/ml
In order to study connexins in retinal glial cells, Invitrogen GJB2 antibody (Zymed, 13-8100) was used in immunohistochemistry on rat samples at 10 ug/ml. J Comp Neurol (2003) ncbi
rabbit polyclonal
  • WB; mouse; fig 2
In order to assess the regional, cellular, and subcellular localization of Cx26 in mouse and rat neural tissue, Invitrogen GJB2 antibody (Zymed, 51-2800) was used in western blot on mouse samples (fig 2). J Comp Neurol (2001) ncbi
mouse monoclonal (CX-12H10)
  • IHC; human
In order to investigate the effects of mutations to connexin-26 on hearing impairment, Invitrogen GJB2 antibody (Zymed, 13-8100) was used in immunohistochemistry on human samples. J Cell Sci (2001) ncbi
mouse monoclonal (CX-12H10)
  • WB; human; fig 3
In order to study intercellular adherens junctions and gap junctions in human gingival fibroblasts, Invitrogen GJB2 antibody (Zymed, CX-12H10) was used in western blot on human samples (fig 3). Am J Physiol Cell Physiol (2000) ncbi
mouse monoclonal (CX-12H10)
  • IHC-P; human; fig 5
  • IHC-P; mouse
  • WB; mouse; 1:300; fig 1
In order to study the role of Cx26 in intravasation and extravasation of tumor cells, Invitrogen GJB2 antibody (Zymed, CX-12H10) was used in immunohistochemistry - paraffin section on human samples (fig 5) and on mouse samples and in western blot on mouse samples at 1:300 (fig 1). J Clin Invest (2000) ncbi
mouse monoclonal (CX-12H10)
  • IHC-F; rat
In order to study the transport function and subcellular localization of mrp6, Invitrogen GJB2 antibody (Zymed, CX 12H10) was used in immunohistochemistry - frozen section on rat samples. Mol Pharmacol (2000) ncbi
Santa Cruz Biotechnology
rabbit polyclonal (O-24)
  • IHC; human; 1:500
Santa Cruz Biotechnology GJB2 antibody (Santa Cruz Biotechnology, sc-130729) was used in immunohistochemistry on human samples at 1:500. Lung Cancer (2014) ncbi
not indicated
  • IHC; human
Santa Cruz Biotechnology goat polyclonal anti-Cx26 antibody was used to study the localization and expression of cx-26, Bcl-xL, and Bax proteins in colorectal cancerWorld J Gastroenterol (2005) ncbi
Abcam
rabbit polyclonal
  • WB; mouse; 1:500
Abcam GJB2 antibody (Abcam, ab38584) was used in western blot on mouse samples at 1:500. Transgenic Res (2013) ncbi
Sigma-Aldrich
mouse monoclonal (CXN-32)
  • WB; mouse; 1:500
In order to study the regulation of autophagosome biogenesis by connexins, Sigma-Aldrich GJB2 antibody (Sigma-Aldrich, C6344) was used in western blot on mouse samples at 1:500. Nat Cell Biol (2014) ncbi
Articles Reviewed
  1. John J Kelly et al. (2015). "Cx30 exhibits unique characteristics including a long half-life when assembled into gap junctions".PMID 26359304
  2. Min Young Lee et al. (2015). "Mice with conditional deletion of Cx26 exhibit no vestibular phenotype despite secondary loss of Cx30 in the vestibular end organs".PMID 26232528
  3. Rana Mroue et al. (2015). "Asymmetric expression of connexins between luminal epithelial- and myoepithelial- cells is essential for contractile function of the mammary gland".PMID 25500615
  4. Qing Chang et al. (2015). "Timed conditional null of connexin26 in mice reveals temporary requirements of connexin26 in key cochlear developmental events before the onset of hearing".PMID 25251605
  5. Xia Wu et al. (2014). "Reduced expression of Connexin26 and its DNA promoter hypermethylation in the inner ear of mimetic aging rats induced by d-galactose".PMID 25159847
  6. Michael K G Stewart et al. (2014). "Mammary gland specific knockdown of the physiological surge in Cx26 during lactation retains normal mammary gland development and function".PMID 24988191
  7. Seon Sook Han et al. (2014). "RNA sequencing identifies novel markers of non-small cell lung cancer".PMID 24751108
  8. Eloy Bejarano et al. (2014). "Connexins modulate autophagosome biogenesis".PMID 24705551
  9. Felicitas Bosen et al. (2014). "The Clouston syndrome mutation connexin30 A88V leads to hyperproliferation of sebaceous glands and hearing impairments in mice".PMID 24685692
  10. Lukas A Holzer et al. (2014). "Expression of gap junction proteins connexins 26, 30, and 43 in Dupuytren's disease".PMID 24359029
  11. Yohei Takada et al. (2014). "Connexin 26 null mice exhibit spiral ganglion degeneration that can be blocked by BDNF gene therapy".PMID 24333301
  12. W Bautista et al. (2014). "Re-evaluation of connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus".PMID 24313680
  13. Mark J Ableser et al. (2014). "Connexin43 reduces melanoma growth within a keratinocyte microenvironment and during tumorigenesis in vivo".PMID 24297173
  14. Jie Yu et al. (2014). "Reduced connexin 43 in eutopic endometrium and cultured endometrial stromal cells from subjects with endometriosis".PMID 24270393
  15. Jianyong Xiao et al. (2013). "Tanshinone IIA increases the bystander effect of herpes simplex virus thymidine kinase/ganciclovir gene therapy via enhanced gap junctional intercellular communication".PMID 23861780
  16. Michael G Kozoriz et al. (2013). "Cerebral ischemic injury is enhanced in a model of oculodentodigital dysplasia".PMID 23727526
  17. Alessandro Terrinoni et al. (2013). "Role of p63 and the Notch pathway in cochlea development and sensorineural deafness".PMID 23589895
  18. Sabine G√ľnther et al. (2013). "Effects of a murine germ cell-specific knockout of Connexin 43 on Connexin expression in testis and fertility".PMID 23188169
  19. B D Lynn et al. (2011). "Ablation of connexin30 in transgenic mice alters expression patterns of connexin26 and connexin32 in glial cells and leptomeninges".PMID 22098503
  20. Giulia Crispino et al. (2011). "BAAV mediated GJB2 gene transfer restores gap junction coupling in cochlear organotypic cultures from deaf Cx26Sox10Cre mice".PMID 21876744
  21. Manuel Valiente et al. (2011). "Focal adhesion kinase modulates radial glia-dependent neuronal migration through connexin-26".PMID 21832197
  22. Joachim Degen et al. (2011). "Connexin32 can restore hearing in connexin26 deficient mice".PMID 21813206
  23. J I Nagy et al. (2011). "Connexin26 expression in brain parenchymal cells demonstrated by targeted connexin ablation in transgenic mice".PMID 21714813
  24. Kelly K Ball et al. (2011). "Reduced gap junctional communication among astrocytes in experimental diabetes: contributions of altered connexin protein levels and oxidative-nitrosative modifications".PMID 21567444
  25. Xue Zhong Liu et al. (2009). "Digenic inheritance of non-syndromic deafness caused by mutations at the gap junction proteins Cx26 and Cx31".PMID 19050930
  26. Ivana Y Kuo et al. (2008). "Limited intravascular coupling in the rodent brainstem and retina supports a role for glia in regional blood flow".PMID 18925566
  27. Cima Cina et al. (2007). "Expression of connexins in embryonic mouse neocortical development".PMID 17640036
  28. Shoeb Ahmad et al. (2007). "Restoration of connexin26 protein level in the cochlea completely rescues hearing in a mouse model of human connexin30-linked deafness".PMID 17227867
  29. Luiza Kanczuga-Koda et al. (2005). "Connexin 26 correlates with Bcl-xL and Bax proteins expression in colorectal cancer".PMID 15770735
  30. J I Nagy et al. (2003). "Coupling of astrocyte connexins Cx26, Cx30, Cx43 to oligodendrocyte Cx29, Cx32, Cx47: Implications from normal and connexin32 knockout mice".PMID 14603462
  31. Trond Aasen et al. (2003). "The relationship between connexins, gap junctions, tissue architecture and tumour invasion, as studied in a novel in vitro model of HPV-16-associated cervical cancer progression".PMID 12970745
  32. Makoto Nishimura et al. (2003). "Suppression of gap junctional intercellular communication via 5' CpG island methylation in promoter region of E-cadherin gene in endometrial cancer cells".PMID 12896902
  33. Kathleen R Zahs et al. (2003). "Connexin immunoreactivity in glial cells of the rat retina".PMID 12508325
  34. J I Nagy et al. (2001). "Connexin26 in adult rodent central nervous system: demonstration at astrocytic gap junctions and colocalization with connexin30 and connexin43".PMID 11745652
  35. F Rouan et al. (2001). "trans-dominant inhibition of connexin-43 by mutant connexin-26: implications for dominant connexin disorders affecting epidermal differentiation".PMID 11493646
  36. K Ko et al. (2000). "Biochemical and functional characterization of intercellular adhesion and gap junctions in fibroblasts".PMID 10898726
  37. A Ito et al. (2000). "A role for heterologous gap junctions between melanoma and endothelial cells in metastasis".PMID 10791993
  38. J Madon et al. (2000). "Transport function and hepatocellular localization of mrp6 in rat liver".PMID 10692506