This is a review about DNA-PKcs, based on 24 published articles (read how Labome selects the articles), using DNA-PKcs antibody in all methods. It is aimed to help Labome visitors find the most suited DNA-PKcs antibody.
DNA-PKcs synonym: DNA-PKcs; DNAPK; DNPK1; HYRC; HYRC1; IMD26; XRCC7; p350; DNA-PK catalytic subunit; hyper-radiosensitivity of murine scid mutation, complementing 1; p460

Abcam
rabbit polyclonal
  • WB; human
In order to examine the role of NF-kappaB during the human papillomavirus life cycle, Abcam DNA-PKcs antibody (Abcam, Ab18192) was used in western blot on human samples. J Virol (2015) ncbi
rabbit polyclonal
  • WB; human
Abcam DNA-PKcs antibody (Abcam, ab18192) was used in western blot on human samples. Histochem Cell Biol (2014) ncbi
mouse monoclonal (18-2)
  • WB; human; 1:5000; fig s6
Abcam DNA-PKcs antibody (Abcam, ab1832) was used in western blot on human samples at 1:5000 (fig s6). Nat Commun (2014) ncbi
mouse monoclonal (18-2)
  • WB; human
Abcam DNA-PKcs antibody (Abcam, 18-2) was used in western blot on human samples. Nucleic Acids Res (2014) ncbi
mouse monoclonal (18-2)
  • WB; human
Abcam DNA-PKcs antibody (Abcam, ab1832) was used in western blot on human samples. Nucleic Acids Res (2014) ncbi
rabbit polyclonal
  • IC; human
In order to study the role of an amino terminal FANCD2 nuclear localization signal in the coordinated nuclear localization of both FANCD2 and FANCI, Abcam DNA-PKcs antibody (Abcam, ab18192) was used in immunocytochemistry on human samples. PLoS ONE (2013) ncbi
mouse monoclonal (18-2)
  • WB; human
In order to investigate the role of TRF2/RAP1 and DNA-PK at telomeric fusion, Abcam DNA-PKcs antibody (Abcam, 18.2) was used in western blot on human samples. EMBO J (2010) ncbi
rabbit polyclonal
  • IC; human; fig 5
In order to show that camptothecin-induced activation of ATM requires transcription, Abcam DNA-PKcs antibody (Abcam, ab18192) was used in immunocytochemistry on human samples (fig 5). J Biol Chem (2010) ncbi
rabbit polyclonal
  • WB; human; fig 2
In order to investigate the mechanism of proteasome-dependent RPA2 phosphorylation, Abcam DNA-PKcs antibody (abcam, ab18192) was used in western blot on human samples (fig 2). DNA Repair (Amst) (2010) ncbi
not indicated
  • WB; human
  • IC; human
Abcam rabbit polyclonal anti-phosph-DNA-PKcs (Thr2609) antibody was used to carry out western blot analysis and immunostaining assays in order to clarify the activation of DNA-PK by protein phosphatase 6 in response to ionizing radiation.PLoS ONE (2009) ncbi
rabbit polyclonal
  • WB; human
Abcam anti-P-DNA-PK S2056 antibody was used in western blot in order to study the role of TRAIL for activation of DNA damage response pathways with histone H2AX and Chk2 phosphorylations.Mol Cell Biol (2009) ncbi
Santa Cruz Biotechnology
mouse monoclonal (G-4)
  • WB; African green monkey; fig 4a
  • IC; African green monkey; fig 4c
Santa Cruz Biotechnology DNA-PKcs antibody (Santa, sc-5282) was used in western blot on African green monkey samples (fig 4a) and in immunocytochemistry on African green monkey samples (fig 4c). PLoS Pathog (2014) ncbi
mouse monoclonal (G-4)
  • IHC; human; 1:50
Santa Cruz Biotechnology DNA-PKcs antibody (Santa Cruz Biotech, sc-5282) was used in immunohistochemistry on human samples at 1:50. Biochem Biophys Res Commun (2014) ncbi
goat polyclonal (C-19)
  • WB; human
Santa Cruz Biotechnology DNA-PKcs antibody (Santa Cruz, sc-1552) was used in western blot on human samples. PLoS ONE (2014) ncbi
not indicated
  • IP; human
  • IC; human
Santa Cruz Biotechnology mouse monoclonal anti-DNA-PKcs antibody was used to carry out immunoprecipitation and immunostaining assays in order to clarify the activation of DNA-PK by protein phosphatase 6 in response to ionizing radiation.PLoS ONE (2009) ncbi
rabbit polyclonal (H-163)
  • WB; human
Santa Cruz Biotechnology DNA-PKcs (sc-9051) antibody was used in western blot (1:400) and EMSA to identify proteins associated with the osmoregulatory transcription factor TonEBP/OREBPstudyAm J Physiol Renal Physiol (2007) ncbi
Invitrogen
mouse monoclonal (3H6)
  • IC; human; fig 4
In order to study the response to double-stranded breaks at the nucleolar organizer regions, Invitrogen DNA-PKcs antibody (Thermo Scientific, MA5-15813) was used in immunocytochemistry on human samples (fig 4). Genes Dev (2015) ncbi
mouse monoclonal (18-2 + 25-4 + 42-psc)
  • WB; mouse; 1:500
In order to evaluate the role of Atm and DNA-PKcs kinases during chromosomal signal joint formation, Invitrogen DNA-PKcs antibody (Thermo-Fisher, MS-423-P1) was used in western blot on mouse samples at 1:500. Proc Natl Acad Sci U S A (2011) ncbi
mouse monoclonal (18-2 + 25-4 + 42-psc)
  • IC; human; fig 5
In order to show that camptothecin-induced activation of ATM requires transcription, Invitrogen DNA-PKcs antibody (Thermo Scientific, Ab-4) was used in immunocytochemistry on human samples (fig 5). J Biol Chem (2010) ncbi
mouse monoclonal (18-2 + 25-4 + 42-psc)
  • WB; human; fig 2
In order to investigate the mechanism of proteasome-dependent RPA2 phosphorylation, Invitrogen DNA-PKcs antibody (Thermo SCIENTIFIC, Ab-4) was used in western blot on human samples (fig 2). DNA Repair (Amst) (2010) ncbi
not indicated
  • WB; human
  • IC; human
NeoMarkers anti-DNA-PKcs antibody was used to carry out western blot analysis and immunofluorescence assays in order to investigate the effect of adeno-associated virus and adenovirus coinfection on inducing cellular DNA damage response.J Virol (2009) ncbi
GeneTex
rabbit polyclonal
  • IC; human; 1:500
GeneTex DNA-PKcs antibody (Genetex, GTX-24194) was used in immunocytochemistry on human samples at 1:500. Radiother Oncol (2012) ncbi
EMD Millipore
not indicated
  • IC; human
Millipore anti-DNA-PKcs antibody was used to perform western blot and immunofluorescence in order to study IRF-3-dependent innate immunityelife (2012) ncbi
not indicated
  • WB; human
Calbiochem anti-DNA-PK antibody was used in western blot in order to study the role of TRAIL for activation of DNA damage response pathways with histone H2AX and Chk2 phosphorylations.Mol Cell Biol (2009) ncbi
not indicated
  • WB; human
Upstate DNA-PKcs antibody was used in western blot to study androgen receptor in prostate cancer cellsJ Biol Chem (2005) ncbi
Cell Signaling Technology
rabbit polyclonal
  • IC; human
Cell Signaling Technology DNA-PKcs antibody (Cell Signaling Technology, 4602) was used in immunocytochemistry on human samples. Int J Oncol (2015) ncbi
Roche Applied Science
not indicatedRoche DNA-PK kinase assay kit was used to test the DNA-PK activity in cell lysate in order to clarify the activation of DNA-PK by protein phosphatase 6 in response to ionizing radiation.PLoS ONE (2009) ncbi
GE Healthcare Life Biosciences
not indicatedDharmacon DNA-PK siRNA was used to perform Luciferase assay in order to show that chromosomal structural aberrations could be caused by chromosome segregation errors.Science (2011) ncbi
not indicatedDharmacon siRNA targeting DNA-PK was used to silence DNA-PK in order to study the role of TRAIL for activation of DNA damage response pathways with histone H2AX and Chk2 phosphorylations.Mol Cell Biol (2009) ncbi
others
not indicatedKuDos DNA-PK inhibitor NU7026 (10 uM) was used in cell culture in order to identify ataxia-telangiectasia mutated (ATM) as a further component of the complex signalling network of radiation-induced DNA damage in non-targeted bystander cells downstream of ataxia-telangiectasia and Rad3-related (ATR)Cancer Res (2008) ncbi
Articles Reviewed
  1. Marjolein Van Sluis et al. (2015). "A localized nucleolar DNA damage response facilitates recruitment of the homology-directed repair machinery independent of cell cycle stage".PMID 26019174
  2. Yifei Wang et al. (2015). "The cranberry flavonoids PAC DP-9 and quercetin aglycone induce cytotoxicity and cell cycle arrest and increase cisplatin sensitivity in ovarian cancer cells".PMID 25776829
  3. Tomomi Nakahara et al. (2015). "Activation of NF-κB by human papillomavirus 16 E1 limits E1-dependent viral replication through degradation of E1".PMID 25717108
  4. Gregory A Sowd et al. (2014). "SV40 utilizes ATM kinase activity to prevent non-homologous end joining of broken viral DNA replication products".PMID 25474690
  5. Ravi Kanth Rao Saini et al. (2014). "Proteomics of dedifferentiation of SK-N-BE2 neuroblastoma cells".PMID 25450381
  6. Michael Osterman et al. (2014). "Elevated DNA damage response in pancreatic cancer".PMID 25002126
  7. Anne Fernandez-Vidal et al. (2014). "A role for DNA polymerase θ in the timing of DNA replication".PMID 24989122
  8. Pierre Alexandre Vidi et al. (2014). "NuMA promotes homologous recombination repair by regulating the accumulation of the ISWI ATPase SNF2h at DNA breaks".PMID 24753406
  9. Francois Belanger et al. (2014). "A majority of human melanoma cell lines exhibits an S phase-specific defect in excision of UV-induced DNA photoproducts".PMID 24416382
  10. Yi Zhou et al. (2014). "Quantitation of DNA double-strand break resection intermediates in human cells".PMID 24362840
  11. Rebecca A Boisvert et al. (2013). "Coordinate nuclear targeting of the FANCD2 and FANCI proteins via a FANCD2 nuclear localization signal".PMID 24278431
  12. Brian J Ferguson et al. (2012). "DNA-PK is a DNA sensor for IRF-3-dependent innate immunity".PMID 23251783
  13. John S Jarboe et al. (2012). "Kinomic profiling approach identifies Trk as a novel radiation modulator".PMID 22561027
  14. Aniek Janssen et al. (2011). "Chromosome segregation errors as a cause of DNA damage and structural chromosome aberrations".PMID 21960636
  15. Eric J Gapud et al. (2011). "Ataxia telangiectasia mutated (Atm) and DNA-PKcs kinases have overlapping activities during chromosomal signal joint formation".PMID 21245316
  16. Oriane Bombarde et al. (2010). "TRF2/RAP1 and DNA-PK mediate a double protection against joining at telomeric ends".PMID 20407424
  17. Ryo Sakasai et al. (2010). "Transcription-dependent activation of ataxia telangiectasia mutated prevents DNA-dependent protein kinase-mediated cell death in response to topoisomerase I poison".PMID 20304914
  18. Ryo Sakasai et al. (2010). "Proteasome inhibition suppresses DNA-dependent protein kinase activation caused by camptothecin".PMID 19959400
  19. Rachel A Schwartz et al. (2009). "Adeno-associated virus replication induces a DNA damage response coordinated by DNA-dependent protein kinase".PMID 19339345
  20. Jun Mi et al. (2009). "Activation of DNA-PK by ionizing radiation is mediated by protein phosphatase 6".PMID 19198648
  21. Stéphanie Solier et al. (2009). "Death receptor-induced activation of the Chk2- and histone H2AX-associated DNA damage response pathways".PMID 18955500
  22. Susanne Burdak-Rothkamm et al. (2008). "ATM acts downstream of ATR in the DNA damage response signaling of bystander cells".PMID 18757420
  23. Ye Chen et al. (2007). "Proteomic identification of proteins associated with the osmoregulatory transcription factor TonEBP/OREBP: functional effects of Hsp90 and PARP-1".PMID 17148781
  24. Greg L Mayeur et al. (2005). "Ku is a novel transcriptional recycling coactivator of the androgen receptor in prostate cancer cells".PMID 15640154