This is a review about CTCF, based on 42 published articles (read how Labome selects the articles), using CTCF antibody in all methods. It is aimed to help Labome visitors find the most suited CTCF antibody.
CTCF synonym: MRD21; 11 zinc finger transcriptional repressor; 11-zinc finger protein; CTCFL paralog

Knockout validation
Bethylrabbit polyclonal
  • IHC-P; mouse; 1:500
  • ChIP; mouse
  • WB (knockout); mouse; fig 3
Bethyl CTCF antibody (Bethyl, A300-543A) was used in immunohistochemistry - paraffin section on mouse samples at 1:500, in chromatin immunoprecipitation on mouse samples, and in western blot (knockout) on mouse samples (fig 3). Dev Cell (2010) ncbi
EMD Millipore
rabbit polyclonal
  • ChIP-Seq; mouse
  • WB; mouse; 1:2000
EMD Millipore CTCF antibody (Millipore, 07-729) was used in ChIP-Seq on mouse samples and in western blot on mouse samples at 1:2000. Proc Natl Acad Sci U S A (2015) ncbi
rabbit polyclonal
  • ChIP; human; fig 10
EMD Millipore CTCF antibody (Millipore, 07-729) was used in chromatin immunoprecipitation on human samples (fig 10). PLoS Pathog (2015) ncbi
rabbit polyclonal
  • ChIP; mouse
In order to study the effect of pulsatile hormone stimulations on gene and regulatory element activation, EMD Millipore CTCF antibody (Millipore, 07-729) was used in chromatin immunoprecipitation on mouse samples. Genome Res (2015) ncbi
rabbit polyclonal
  • IP; human
EMD Millipore CTCF antibody (Upstate, 07-729) was used in immunoprecipitation on human samples. Stem Cell Reports (2014) ncbi
rabbit polyclonal
  • ChIP; human
EMD Millipore CTCF antibody (Millipore, 07-729) was used in chromatin immunoprecipitation on human samples. Nucleic Acids Res (2014) ncbi
rabbit polyclonal
  • WB; human; 1:1000
EMD Millipore CTCF antibody (Millipore, 07-729) was used in western blot on human samples at 1:1000. PLoS ONE (2014) ncbi
rabbit polyclonal
  • ChIP; human
EMD Millipore CTCF antibody (Millipore, 07-729) was used in chromatin immunoprecipitation on human samples. Mol Cell Biol (2014) ncbi
rabbit polyclonal
  • ChIP; mouse
EMD Millipore CTCF antibody (Millipore, 07-729) was used in chromatin immunoprecipitation on mouse samples. Genes Immun (2014) ncbi
rabbit polyclonal
  • ChIP; human
  • WB; human
EMD Millipore CTCF antibody (Millipore, 07-729) was used in chromatin immunoprecipitation on human samples and in western blot on human samples. Nucleic Acids Res (2014) ncbi
rabbit polyclonal
  • ChIP; human
EMD Millipore CTCF antibody (Millipore, 07-729) was used in chromatin immunoprecipitation on human samples. PLoS ONE (2014) ncbi
rabbit polyclonal
  • ChIP-Seq; human
EMD Millipore CTCF antibody (Millipore, 07-729) was used in ChIP-Seq on human samples. Nucleic Acids Res (2014) ncbi
rabbit polyclonal
  • ChIP; human
EMD Millipore CTCF antibody (Millipore, 07-729) was used in chromatin immunoprecipitation on human samples. Gene (2014) ncbi
rabbit polyclonal
  • WB; human; 1:2000
EMD Millipore CTCF antibody (Millipore, 07-729) was used in western blot on human samples at 1:2000. Mol Cell Biol (2014) ncbi
rabbit polyclonal
  • ChIP; human
EMD Millipore CTCF antibody (Millipore, 07-729) was used in chromatin immunoprecipitation on human samples. Epigenetics Chromatin (2014) ncbi
rabbit polyclonal
  • ChIP; human
  • WB; human
  • WB; mouse
EMD Millipore CTCF antibody (Millipore, 07-729) was used in chromatin immunoprecipitation on human samples and in western blot on human samples and on mouse samples. PLoS ONE (2014) ncbi
rabbit polyclonal
  • IC; human
EMD Millipore CTCF antibody (Millipore, 07-729) was used in immunocytochemistry on human samples. PLoS Genet (2014) ncbi
rabbit polyclonal
  • WB; mouse; 1:5000
EMD Millipore CTCF antibody (Upstate, 07-729) was used in western blot on mouse samples at 1:5000. PLoS ONE (2014) ncbi
rabbit polyclonal
  • ChIP; mouse
EMD Millipore CTCF antibody (Upstate, 07?C729) was used in chromatin immunoprecipitation on mouse samples. PLoS ONE (2014) ncbi
rabbit polyclonal
  • ChIP; human
EMD Millipore CTCF antibody (Millipore, 07-729) was used in chromatin immunoprecipitation on human samples. Mol Oncol (2014) ncbi
rabbit polyclonal
  • ChIP-Seq; human
EMD Millipore CTCF antibody (Millipore, 07-729) was used in ChIP-Seq on human samples. Nat Biotechnol (2014) ncbi
rabbit polyclonal
  • ChIP; mouse; 10 ul
In order to study the roles of Brg1, Chd4 and Snf2h in genome-wide remodeling of chromatin, EMD Millipore CTCF antibody (Millipore, 07-729) was used in chromatin immunoprecipitation on mouse samples at 10 ul. Nat Struct Mol Biol (2014) ncbi
rabbit polyclonal
  • WB; human; 1:1000
EMD Millipore CTCF antibody (Millipore, 07-729) was used in western blot on human samples at 1:1000. BMC Cell Biol (2013) ncbi
rabbit polyclonal
  • ChIP-Seq; human
EMD Millipore CTCF antibody (Millipore, 07-729) was used in ChIP-Seq on human samples. Nucleic Acids Res (2014) ncbi
rabbit polyclonal
  • ChIP-Seq; mouse
EMD Millipore CTCF antibody (Millipore, 07-729) was used in ChIP-Seq on mouse samples. Nucleic Acids Res (2014) ncbi
rabbit polyclonal
  • ChIP; mouse
EMD Millipore CTCF antibody (Millipore, 07-729) was used in chromatin immunoprecipitation on mouse samples. Mol Cell Biol (2013) ncbi
rabbit polyclonal
  • IHC; human
  • WB; human; 1:2000
EMD Millipore CTCF antibody (Millipore, 07-729) was used in immunohistochemistry on human samples and in western blot on human samples at 1:2000. Front Oncol (2013) ncbi
rabbit polyclonal
  • ChIP; human; 20 ul
EMD Millipore CTCF antibody (Millipore, 07-729) was used in chromatin immunoprecipitation on human samples at 20 ul. Mol Cell Biol (2013) ncbi
rabbit polyclonal
  • ChIP; human
  • WB; human
EMD Millipore CTCF antibody (Millipore, 07-729) was used in chromatin immunoprecipitation on human samples and in western blot on human samples. J Biol Chem (2013) ncbi
rabbit polyclonal
  • ChIP; human
  • ChIP; mouse
EMD Millipore CTCF antibody (Millipore, 07-729) was used in chromatin immunoprecipitation on human samples and on mouse samples. J Immunol (2013) ncbi
rabbit polyclonal
  • ChIP; mouse
EMD Millipore CTCF antibody (Millipore, 07-729) was used in chromatin immunoprecipitation on mouse samples. Nucleic Acids Res (2013) ncbi
not indicated
  • WB; human
Upstate rabbit polyclonal Ab anti-Ctcf was used in western blot in order to show that Parp1 and ADP-ribose polymers localize on the Dnmt1 promoter and Parp1 can protect its unmethylated state.PLoS ONE (2009) ncbi
not indicated
  • IP; human
Upstate Biotechnologies rabbit polyclonal anti-CTCF antibody was used in immunoprecipitation in order to understand the molecular mechanisms leading to FSHDPLoS Genet (2009) ncbi
not indicated
  • EMSA; human
  • ChIP; human
Upstate anti-CTCF antibody (07-729) was used in ChIP assay and EMSA to study regulation of expression of the CFTR geneNucleic Acids Res (2009) ncbi
not indicated
  • ChIP; human
Upstate Biotechnology anti-CTCF antibody was used in ChIP assays in order to study the role of CTCF in sister chromatid cohesionProc Natl Acad Sci U S A (2008) ncbi
not indicated
  • ChIP; human
Upstate(Millipore) anti-human CTCF antibody (Catalog #,07729)was used to perform ChIP assays in order to study the functional mechanisms of interaction between cohesins and CTCF on mammalian chromosome arms.Cell (2008) ncbi
not indicated
  • WB; human
  • IP; human
Upstate Biotechnology rabbit anti-CTCF polyclonal antibody (catalog no. 06-917) was used in western blot and immunoprecipitation to study the interaction between human enhancer blocker CTC-binding factor and transcription factor KaisoJ Biol Chem (2005) ncbi
Abcam
rabbit polyclonal
  • ChIP; human
Abcam CTCF antibody (Abcam, ab70303) was used in chromatin immunoprecipitation on human samples. Am J Hum Genet (2015) ncbi
rabbit polyclonal
  • WB; mouse; fig 6c
Abcam CTCF antibody (Abcam, Ab70303) was used in western blot on mouse samples (fig 6c). elife (2014) ncbi
not indicated
  • WB; human
Abcam anti-CTCF polyclonal antibody was used in western blot to study the growth and erythroid differentiation of human myeloid leukemia cellsJ Biol Chem (2005) ncbi
Santa Cruz Biotechnology
goat polyclonal (C-20)
  • IP; mouse
Santa Cruz Biotechnology CTCF antibody (Santa, SC15914) was used in immunoprecipitation on mouse samples. Proc Natl Acad Sci U S A (2015) ncbi
rabbit polyclonal (H-280)
  • IHC; mouse; 1:500
Santa Cruz Biotechnology CTCF antibody (Santa Cruz, sc-28198) was used in immunohistochemistry on mouse samples at 1:500. PLoS ONE (2014) ncbi
not indicated
  • IP; human
Santa Cruz Biotechnology anti-CTCF antibody was used to perform immunoprecipitation in order to demonstrate that physical barrier due to the folding of chromatin in domain II can give rise to the depletion of RNA polymerase II.Mol Cell Biol (2009) ncbi
Bethyl
rabbit polyclonal
  • IHC-P; mouse; 1:500
  • ChIP; mouse
  • WB (knockout); mouse; fig 3
Bethyl CTCF antibody (Bethyl, A300-543A) was used in immunohistochemistry - paraffin section on mouse samples at 1:500, in chromatin immunoprecipitation on mouse samples, and in western blot (knockout) on mouse samples (fig 3). Dev Cell (2010) ncbi
Cell Signaling Technology
rabbit polyclonal
  • WB; human; fig 9
  • IC; human; 1:50; fig 7
Cell Signaling Technology CTCF antibody (Cell Signaling, 2899) was used in western blot on human samples (fig 9) and in immunocytochemistry on human samples at 1:50 (fig 7). PLoS Pathog (2015) ncbi
rabbit polyclonal
  • ChIP-Seq; human; fig 4
Cell Signaling Technology CTCF antibody (Cell Signaling, 2899) was used in ChIP-Seq on human samples (fig 4). Nature (2015) ncbi
BD Biosciences
mouse monoclonal (48/CTCF)
  • WB; human; fig 9
  • IC; human; 1:50; fig 7
BD Biosciences CTCF antibody (BD, 612149) was used in western blot on human samples (fig 9) and in immunocytochemistry on human samples at 1:50 (fig 7). PLoS Pathog (2015) ncbi
mouse monoclonal (48/CTCF)
  • IC; human
BD Biosciences CTCF antibody (BD, 612149) was used in immunocytochemistry on human samples. PLoS Genet (2014) ncbi
not indicated
  • WB; human
BD Biosciences anti-CTCF monoclonal antibody was used in western blot to study the growth and erythroid differentiation of human myeloid leukemia cellsJ Biol Chem (2005) ncbi
GE Healthcare Life Biosciences
not indicatedDharmacon human CTCF (M-020165-01) was used in RNA Interference in order to understand the molecular mechanisms leading to FSHDPLoS Genet (2009) ncbi
Articles Reviewed
  1. Kelan Chen et al. (2015). "Genome-wide binding and mechanistic analyses of Smchd1-mediated epigenetic regulation".PMID 26091879
  2. Kavi Mehta et al. (2015). "Human papillomaviruses activate and recruit SMC1 cohesin proteins for the differentiation-dependent life cycle through association with CTCF insulators".PMID 25875106
  3. Diana A Stavreva et al. (2015). "Dynamics of chromatin accessibility and long-range interactions in response to glucocorticoid pulsing".PMID 25677181
  4. Amita Singh et al. (2015). "TFIIH subunit alterations causing xeroderma pigmentosum and trichothiodystrophy specifically disturb several steps during transcription".PMID 25620205
  5. Eliezer Calo et al. (2015). "RNA helicase DDX21 coordinates transcription and ribosomal RNA processing".PMID 25470060
  6. Michal Avitzour et al. (2014). "FMR1 epigenetic silencing commonly occurs in undifferentiated fragile X-affected embryonic stem cells".PMID 25418717
  7. Oliver Weth et al. (2014). "CTCF induces histone variant incorporation, erases the H3K27me3 histone mark and opens chromatin".PMID 25294833
  8. Justyna Zaborowska et al. (2014). "Herpes Simplex Virus 1 (HSV-1) ICP22 protein directly interacts with cyclin-dependent kinase (CDK)9 to inhibit RNA polymerase II transcription elongation".PMID 25233083
  9. Miao Xu et al. (2014). "CTCF controls HOXA cluster silencing and mediates PRC2-repressive higher-order chromatin structure in NT2/D1 cells".PMID 25135475
  10. S Lohsen et al. (2014). "Common distal elements orchestrate CIITA isoform-specific expression in multiple cell types".PMID 25101797
  11. Nehal Gosalia et al. (2014). "Architectural proteins CTCF and cohesin have distinct roles in modulating the higher order structure and expression of the CFTR locus".PMID 25081205
  12. Cassandra Mostocotto et al. (2014). "Poly(ADP-ribosyl)ation is required to modulate chromatin changes at c-MYC promoter during emergence from quiescence".PMID 25047032
  13. Kyoko Matsumoto et al. (2014). "Construction of mate pair full-length cDNAs libraries and characterization of transcriptional start sites and termination sites".PMID 25034687
  14. Sowmya Jairam et al. (2014). "An enhancer-blocking element regulates the cell-specific expression of alcohol dehydrogenase 7".PMID 24971505
  15. Tomasz Chelmicki et al. (2014). "MOF-associated complexes ensure stem cell identity and Xist repression".PMID 24842875
  16. Justine Quintin et al. (2014). "Dynamic estrogen receptor interactomes control estrogen-responsive trefoil Factor (TFF) locus cell-specific activities".PMID 24752895
  17. Franck Court et al. (2014). "The PEG13-DMR and brain-specific enhancers dictate imprinted expression within the 8q24 intellectual disability risk locus".PMID 24667089
  18. Bing Yang et al. (2014). "A novel pathway links oxidative stress to loss of insulin growth factor-2 (IGF2) imprinting through NF-κB activation".PMID 24558376
  19. Jessica Zuin et al. (2014). "A cohesin-independent role for NIPBL at promoters provides insights in CdLS".PMID 24550742
  20. Martina Dluhosova et al. (2014). "Epigenetic control of SPI1 gene by CTCF and ISWI ATPase SMARCA5".PMID 24498324
  21. Pascale Hermant et al. (2014). "Human but not mouse hepatocytes respond to interferon-lambda in vivo".PMID 24498220
  22. David A Quigley et al. (2014). "The 5p12 breast cancer susceptibility locus affects MRPS30 expression in estrogen-receptor positive tumors".PMID 24388359
  23. Lars Anders et al. (2014). "Genome-wide localization of small molecules".PMID 24336317
  24. Stephanie A Morris et al. (2014). "Overlapping chromatin-remodeling systems collaborate genome wide at dynamic chromatin transitions".PMID 24317492
  25. Babatunji W Ogunkolade et al. (2013). "BORIS/CTCFL is an RNA-binding protein that associates with polysomes".PMID 24279897
  26. Mathieu Rousseau et al. (2014). "Hox in motion: tracking HoxA cluster conformation during differentiation".PMID 24174538
  27. Robert N Plasschaert et al. (2014). "CTCF binding site sequence differences are associated with unique regulatory and functional trends during embryonic stem cell differentiation".PMID 24121688
  28. Joanna R DiSpirito et al. (2013). "Pruning of the adipocyte peroxisome proliferator-activated receptor γ cistrome by hematopoietic master regulator PU.1".PMID 23775123
  29. Zhiqing Huang et al. (2013). "Increased Intragenic IGF2 Methylation is Associated with Repression of Insulator Activity and Elevated Expression in Serous Ovarian Carcinoma".PMID 23745176
  30. Da Hai Yu et al. (2013). "Developmentally programmed 3' CpG island methylation confers tissue- and cell-type-specific transcriptional activation".PMID 23459939
  31. Nehal Gosalia et al. (2013). "Coordinate regulation of the gel-forming mucin genes at chromosome 11p15.5".PMID 23303185
  32. Yougui Xiang et al. (2013). "Vκ gene repertoire and locus contraction are specified by critical DNase I hypersensitive sites within the Vκ-Jκ intervening region".PMID 23296705
  33. Isabel Iglesias-Platas et al. (2013). "Imprinting at the PLAGL1 domain is contained within a 70-kb CTCF/cohesin-mediated non-allelic chromatin loop".PMID 23295672
  34. Natalia Soshnikova et al. (2010). "Functional analysis of CTCF during mammalian limb development".PMID 21145498
  35. Michele Zampieri et al. (2009). "Parp1 localizes within the Dnmt1 promoter and protects its unmethylated state by its enzymatic activity".PMID 19262751
  36. Alexandre Ottaviani et al. (2009). "The D4Z4 macrosatellite repeat acts as a CTCF and A-type lamins-dependent insulator in facio-scapulo-humeral dystrophy".PMID 19247430
  37. Neil P Blackledge et al. (2009). "An insulator element 3' to the CFTR gene binds CTCF and reveals an active chromatin hub in primary cells".PMID 19129223
  38. Chia Hsin Su et al. (2009). "p53 chromatin epigenetic domain organization and p53 transcription".PMID 18936172
  39. Eric D Rubio et al. (2008). "CTCF physically links cohesin to chromatin".PMID 18550811
  40. Vania Parelho et al. (2008). "Cohesins functionally associate with CTCF on mammalian chromosome arms".PMID 18237772
  41. Pierre Antoine Defossez et al. (2005). "The human enhancer blocker CTC-binding factor interacts with the transcription factor Kaiso".PMID 16230345
  42. Verónica Torrano et al. (2005). "CTCF regulates growth and erythroid differentiation of human myeloid leukemia cells".PMID 15941718