This is a review about BACE, based on 25 published articles (read how Labome selects the articles), using BACE antibody in all methods. It is aimed to help Labome visitors find the most suited BACE antibody.
BACE synonym: ASP2; BACE; HSPC104; APP beta-secretase; asp 2; aspartyl protease 2; beta-secretase 1 precursor variant 1; beta-site APP cleaving enzyme 1; beta-site amyloid beta A4 precursor protein-cleaving enzyme; memapsin-2; membrane-associated aspartic protease 2; transmembrane aspartic proteinase Asp2

Knockout validation
Cell Signaling Technologyrabbit monoclonal (D10E5)
  • IHC-F; mouse; fig 5d
  • WB (knockout); mouse; fig 5a
  • IP; human; fig 2b
  • IP; mouse; fig 1b
  • WB; human; fig 2b
In order to study the effect of bisecting N-acetylglucosamine on beta-site amyloid precursor protein cleaving enzyme-1 (BACE1) activity, Cell Signaling Technology BACE antibody (Cell Signaling Technology, 5606) was used in immunohistochemistry - frozen section on mouse samples (fig 5d), in western blot (knockout) on mouse samples (fig 5a), and in immunoprecipitation on human samples (fig 2b) and on mouse samples (fig 1b). EMBO Mol Med (2015) ncbi
EMD Millipore
rabbit polyclonal
  • WB; mouse; 1:400
EMD Millipore BACE antibody (Merck Millipore, AB-5832) was used in western blot on mouse samples at 1:400. Oxid Med Cell Longev (2015) ncbi
rabbit polyclonal
  • WB; mouse; 1:500; fig 6
In order to characterize cognitive and neuropathological Alzheimer's disease markers in a novel mouse model, EMD Millipore BACE antibody (Millipore, AB5832) was used in western blot on mouse samples at 1:500 (fig 6). Age (Dordr) (2015) ncbi
rabbit polyclonal
  • WB; human; 1:500
In order to investigate the role of repressor element 1-silencing transcription factor in neurodegeneration during ageing, EMD Millipore BACE antibody (Millipore, AB5832) was used in western blot on human samples at 1:500. Nature (2014) ncbi
rabbit polyclonal
  • WB; mouse
In order to study Abeta agggregation and oligomerization induced in a murine model of Alzheimer's disease by experimental traumatic brain injury, EMD Millipore BACE antibody (Millipore, AB5940) was used in western blot on mouse samples. J Neurotrauma (2014) ncbi
rabbit polyclonal
  • WB; rat
  • IC; rat; 1:200
EMD Millipore BACE antibody (Millipore, AB5940) was used in western blot on rat samples and in immunocytochemistry on rat samples at 1:200. BMC Complement Altern Med (2012) ncbi
not indicated
  • WB; human
Chemicon monoclonal anti-BACE antibody was used in western blot in order to study the role of NGF or BDNF signaling in neuronal apoptotic deathProc Natl Acad Sci U S A (2008) ncbi
rabbit polyclonal
  • IHC-F; rat; 1:400
  • WB; rat; 1:1000
EMD Millipore BACE antibody (Chemicon, AB5940) was used in immunohistochemistry - frozen section on rat samples at 1:400 and in western blot on rat samples at 1:1000. J Comp Neurol (2007) ncbi
not indicated
  • WB; human
Calbiochem rabbit antibodies recognizing the proregion of immature BACE (AA 26 45) and the NH2-terminal end of mature BACE (AA 46 65) were used in western blot to study lysosomal BACE degradationJ Biol Chem (2005) ncbi
Abcam
rabbit polyclonal
  • WB; human; 1:1000
In order to examine the importance of iron in relation to amyloid-beta production within the retina, Abcam BACE antibody (Abcam, ab2077) was used in western blot on human samples at 1:1000. Exp Eye Res (2014) ncbi
rabbit polyclonal
  • WB; rat
Abcam BACE antibody (Abcam, ab2077) was used in western blot on rat samples. Exp Neurol (2013) ncbi
rabbit polyclonal
  • WB; mouse; 1 ug/ml
In order to test if proprotein convertase subtilisin/kexin type 9 modulates the levels of low-density lipoprotein receptor and other membrane-bound proteins in the adult mouse brain, Abcam BACE antibody (Abcam, ab2077) was used in western blot on mouse samples at 1 ug/ml. J Lipid Res (2010) ncbi
Santa Cruz Biotechnology
rabbit polyclonal (M-83)
  • WB; mouse
In order to study the beneficial effects of S100A9 deletion on memory and beta-amyloid pathology in a murine model of Alzheimer's disease, Santa Cruz Biotechnology BACE antibody (Santa Cruz Biotechnology, sc-10748) was used in western blot on mouse samples. PLoS ONE (2014) ncbi
goat polyclonal (D-16)
  • WB; mouse
Santa Cruz Biotechnology BACE antibody (Santa Cruz Biotechnology, sc-10053) was used in western blot on mouse samples. J Neural Transm (Vienna) (2014) ncbi
not indicated
  • WB; human
Santa Cruz anti-BACE1 was used in 1:1,000 dilution to perform western blot in order to show that new BACE1 substrates were identified by quantitative proteomics.PLoS ONE (2009) ncbi
not indicated
  • WB; human
Santa Cruz Biotechnology polyclonal BACE antibody (1:200) was used for western blot in order to indentify new biomarker of Alzheimer's disease.PLoS ONE (2009) ncbi
Invitrogen
rabbit polyclonal
  • WB; human
Invitrogen BACE antibody (Thermo Scientific, PA1?C757) was used in western blot on human samples. Biochemistry (2015) ncbi
not indicated
  • WB; human
Affinity BioReagents anti-BACE1 antibody (PA1 757) was used in human cells and in western blot to study the role for ubiquilin 1 in modulating amyloid precursor protein trafficking and Abeta secretion.J Biol Chem (2006) ncbi
not indicated
  • WB; human
Affinity Bioreagents rabbit anti-BACE COOH-terminal antibody was used in western blot to study lysosomal BACE degradationJ Biol Chem (2005) ncbi
Epitomics
rabbit monoclonal
  • WB; mouse; 1:1000
Epitomics BACE antibody (Epitomics, 2882?C1) was used in western blot on mouse samples at 1:1000. Proc Natl Acad Sci U S A (2015) ncbi
BioLegend
rabbit polyclonal (Poly8401)
  • IHC-F; rat; 1:2000
  • WB; rat; 1:5000
BioLegend BACE antibody (Convance, PRB-617c) was used in immunohistochemistry - frozen section on rat samples at 1:2000 and in western blot on rat samples at 1:5000. J Comp Neurol (2007) ncbi
ProSci
rabbit polyclonal
  • WB; human
ProSci BACE antibody (Prosci Inc., 2253) was used in western blot on human samples. Neurobiol Dis (2009) ncbi
Cell Signaling Technology
rabbit monoclonal (D10E5)
  • IP; mouse
  • WB; mouse
  • IC; mouse
In order to test if bisecting GlcNAc would stabilize BACE1 protein upon oxidative stress, Cell Signaling Technology BACE antibody (Cell Signaling Technology, 5606) was used in immunoprecipitation on mouse samples, in western blot on mouse samples, and in immunocytochemistry on mouse samples. Biochem J (2016) ncbi
rabbit monoclonal (D10E5)
  • IHC; mouse; 1:400
  • IC; mouse; 1:500
Cell Signaling Technology BACE antibody (Cell signaling, 5606) was used in immunohistochemistry on mouse samples at 1:400 and in immunocytochemistry on mouse samples at 1:500. Neurobiol Aging (2015) ncbi
rabbit monoclonal (D10E5)
  • IHC-F; mouse; fig 5d
  • WB (knockout); mouse; fig 5a
  • IP; human; fig 2b
  • IP; mouse; fig 1b
  • WB; human; fig 2b
In order to study the effect of bisecting N-acetylglucosamine on beta-site amyloid precursor protein cleaving enzyme-1 (BACE1) activity, Cell Signaling Technology BACE antibody (Cell Signaling Technology, 5606) was used in immunohistochemistry - frozen section on mouse samples (fig 5d), in western blot (knockout) on mouse samples (fig 5a), and in immunoprecipitation on human samples (fig 2b) and on mouse samples (fig 1b). EMBO Mol Med (2015) ncbi
rabbit monoclonal (D10E5)
  • IHC-F; mouse; 1:500
Cell Signaling Technology BACE antibody (Cell Signaling, D10E5) was used in immunohistochemistry - frozen section on mouse samples at 1:500. PLoS ONE (2014) ncbi
rabbit monoclonal (D10E5)
  • WB; mouse; 1:1000; fig 3
In order to investigate the contribution of IL-12/23 to disease using SAMP8 mice, Cell Signaling Technology BACE antibody (Cell Signaling Technology, #5606) was used in western blot on mouse samples at 1:1000 (fig 3). J Alzheimers Dis (2014) ncbi
Peptide Institute
not indicatedPeptide Institute aspartic protease inhibitors (Z-LL) 2 ketone was used to treat cells in order to study the role for intramembrane processing by signal peptide peptidase in regulating the membrane localization of hepatitis C virus core protein and viral propagation.J Virol (2008) ncbi
not indicatedPeptide Institute aspartic protease inhibitors L685, 458 was used to treat cells in order to study the role for intramembrane processing by signal peptide peptidase in regulating the membrane localization of hepatitis C virus core protein and viral propagation.J Virol (2008) ncbi
Articles Reviewed
  1. Yasuhiko Kizuka et al. (2016). "Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions".PMID 26467158
  2. Anke Schloesser et al. (2015). "Dietary Tocotrienol/γ-Cyclodextrin Complex Increases Mitochondrial Membrane Potential and ATP Concentrations in the Brains of Aged Mice".PMID 26301044
  3. Grant T Corbett et al. (2015). "Activation of peroxisome proliferator-activated receptor α stimulates ADAM10-mediated proteolysis of APP".PMID 26080426
  4. Haizhi Wang et al. (2015). "Cathepsin L Mediates the Degradation of Novel APP C-Terminal Fragments".PMID 25910068
  5. Hongyun Li et al. (2015). "Apolipoprotein D modulates amyloid pathology in APP/PS1 Alzheimer's disease mice".PMID 25784209
  6. D Porquet et al. (2015). "Amyloid and tau pathology of familial Alzheimer's disease APP/PS1 mouse model in a senescence phenotype background (SAMP8)".PMID 25663420
  7. Yasuhiko Kizuka et al. (2015). "An aberrant sugar modification of BACE1 blocks its lysosomal targeting in Alzheimer's disease".PMID 25592972
  8. Lucie Y Guo et al. (2014). "Iron increases APP translation and amyloid-beta production in the retina".PMID 25456519
  9. Katherine R Sadleir et al. (2014). "Genetic inhibition of phosphorylation of the translation initiation factor eIF2α does not block Aβ-dependent elevation of BACE1 and APP levels or reduce amyloid pathology in a mouse model of Alzheimer's disease".PMID 24992504
  10. Tao Lu et al. (2014). "REST and stress resistance in ageing and Alzheimer's disease".PMID 24670762
  11. Hee Jin Kim et al. (2014). "S100A9 knockout decreases the memory impairment and neuropathology in crossbreed mice of Tg2576 and S100A9 knockout mice model".PMID 24586443
  12. Eduardo Luiz Gasnhar Moreira et al. (2014). "Hypercholesterolemia induces short-term spatial memory impairments in mice: up-regulation of acetylcholinesterase activity as an early and causal event?".PMID 24166183
  13. Patricia M Washington et al. (2014). "Experimental traumatic brain injury induces rapid aggregation and oligomerization of amyloid-beta in an Alzheimer's disease mouse model".PMID 24050316
  14. Meng Shan Tan et al. (2014). "IL12/23 p40 inhibition ameliorates Alzheimer's disease-associated neuropathology and spatial memory in SAMP8 mice".PMID 24047617
  15. Li Liu et al. (2013). "Palmitate induces transcriptional regulation of BACE1 and presenilin by STAT3 in neurons mediated by astrocytes".PMID 23968646
  16. Ju fang Huang et al. (2012). "Timosaponin-BII inhibits the up-regulation of BACE1 induced by ferric chloride in rat retina".PMID 23082924
  17. Mali Liu et al. (2010). "PCSK9 is not involved in the degradation of LDL receptors and BACE1 in the adult mouse brain".PMID 20453200
  18. Matthew L Hemming et al. (2009). "Identification of beta-secretase (BACE1) substrates using quantitative proteomics".PMID 20041192
  19. Alessio Colombo et al. (2009). "JNK regulates APP cleavage and degradation in a model of Alzheimer's disease".PMID 19166938
  20. Weiping Qin et al. (2009). "S100A7, a novel Alzheimer's disease biomarker with non-amyloidogenic alpha-secretase activity acts via selective promotion of ADAM-10".PMID 19159013
  21. Carmela Matrone et al. (2008). "NGF and BDNF signaling control amyloidogenic route and Abeta production in hippocampal neurons".PMID 18728191
  22. Kiyoko Okamoto et al. (2008). "Intramembrane processing by signal peptide peptidase regulates the membrane localization of hepatitis C virus core protein and viral propagation".PMID 18562515
  23. Xiao Xin Yan et al. (2007). "beta-Secretase expression in normal and functionally deprived rat olfactory bulbs: inverse correlation with oxidative metabolic activity".PMID 17206602
  24. Mikko Hiltunen et al. (2006). "Ubiquilin 1 modulates amyloid precursor protein trafficking and Abeta secretion".PMID 16945923
  25. Young Ho Koh et al. (2005). "BACE is degraded via the lysosomal pathway".PMID 16033761