product summary
request information :
company name :
eBioscience
other brands :
NatuTec
product type :
antibody
product name :
Anti-Human/Mouse ROR gamma (t) Purified 25 ug
catalog :
14-6988-80
quantity :
25 ug
price :
139.00 USD
clonality :
monoclonal
host :
rat
reactivity :
human, mouse
application :
western blot, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 53
Published Application/Species/DilutionReference
  • flow cytometry; mouse
Weitkamp J, Rosen M, Zhao Z, Koyama T, Geem D, Denning T, et al. Small intestinal intraepithelial TCRγδ+ T lymphocytes are present in the premature intestine but selectively reduced in surgical necrotizing enterocolitis. PLoS ONE. 2014;9:e99042 pubmed publisher
  • flow cytometry; mouse; 1:200
Chatterjee S, Thyagarajan K, Kesarwani P, Song J, Soloshchenko M, Fu J, et al. Reducing CD73 expression by IL1β-Programmed Th17 cells improves immunotherapeutic control of tumors. Cancer Res. 2014;74:6048-59 pubmed publisher
  • flow cytometry; mouse
  • immunocytochemistry; mouse
McGuire D, Rowse A, Li H, Peng B, Sestero C, Cashman K, et al. CD5 enhances Th17-cell differentiation by regulating IFN-γ response and RORγt localization. Eur J Immunol. 2014;44:1137-42 pubmed publisher
  • flow cytometry; mouse
Pick J, Arra A, Lingel H, Hegel J, Huber M, Nishanth G, et al. CTLA-4 (CD152) enhances the Tc17 differentiation program. Eur J Immunol. 2014;44:2139-52 pubmed publisher
  • immunohistochemistry - frozen section; mouse
  • flow cytometry; human
Longman R, Diehl G, Victorio D, Huh J, Galan C, Miraldi E, et al. CX₃CR1⁺ mononuclear phagocytes support colitis-associated innate lymphoid cell production of IL-22. J Exp Med. 2014;211:1571-83 pubmed publisher
  • immunocytochemistry; human; tbl 1
Magri G, Miyajima M, Bascones S, Mortha A, Puga I, Cassis L, et al. Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells. Nat Immunol. 2014;15:354-64 pubmed publisher
  • western blot; human
Han L, Yang J, Wang X, Wu Q, Yin S, Li Z, et al. The E3 deubiquitinase USP17 is a positive regulator of retinoic acid-related orphan nuclear receptor γt (RORγt) in Th17 cells. J Biol Chem. 2014;289:25546-55 pubmed publisher
Edwards J, Fujii H, Zhou A, Creemers J, Unutmaz D, Shevach E. Regulation of the expression of GARP/latent TGF-β1 complexes on mouse T cells and their role in regulatory T cell and Th17 differentiation. J Immunol. 2013;190:5506-15 pubmed publisher
Pinchuk I, Morris K, Nofchissey R, Earley R, Wu J, Ma T, et al. Stromal cells induce Th17 during Helicobacter pylori infection and in the gastric tumor microenvironment. PLoS ONE. 2013;8:e53798 pubmed publisher
Gironi M, Saresella M, Rovaris M, Vaghi M, Nemni R, Clerici M, et al. A novel data mining system points out hidden relationships between immunological markers in multiple sclerosis. Immun Ageing. 2013;10:1 pubmed publisher
Lina T, Pinchuk I, House J, Yamaoka Y, Graham D, Beswick E, et al. CagA-dependent downregulation of B7-H2 expression on gastric mucosa and inhibition of Th17 responses during Helicobacter pylori infection. J Immunol. 2013;191:3838-46 pubmed publisher
Kleinewietfeld M, Manzel A, Titze J, Kvakan H, Yosef N, Linker R, et al. Sodium chloride drives autoimmune disease by the induction of pathogenic TH17 cells. Nature. 2013;496:518-22 pubmed publisher
Kumar P, Thakar M, Ouyang W, Malarkannan S. IL-22 from conventional NK cells is epithelial regenerative and inflammation protective during influenza infection. Mucosal Immunol. 2013;6:69-82 pubmed publisher
McArthur M, Sztein M. Unexpected heterogeneity of multifunctional T cells in response to superantigen stimulation in humans. Clin Immunol. 2013;146:140-52 pubmed publisher
Wong L, HATFIELD J, Brown M. Ikaros sets the potential for Th17 lineage gene expression through effects on chromatin state in early T cell development. J Biol Chem. 2013;288:35170-9 pubmed publisher
Kathania M, Zadeh M, Lightfoot Y, Roman R, Sahay B, Abbott J, et al. Colonic immune stimulation by targeted oral vaccine. PLoS ONE. 2013;8:e55143 pubmed publisher
Mielke L, Groom J, Rankin L, Seillet C, Masson F, Putoczki T, et al. TCF-1 controls ILC2 and NKp46+RORγt+ innate lymphocyte differentiation and protection in intestinal inflammation. J Immunol. 2013;191:4383-91 pubmed publisher
Monk J, Hou T, Turk H, McMurray D, Chapkin R. n3 PUFAs reduce mouse CD4+ T-cell ex vivo polarization into Th17 cells. J Nutr. 2013;143:1501-8 pubmed publisher
Peters J, Tjabringa G, Fasse E, de Oliveira V, Schalkwijk J, Koenen H, et al. Co-culture of healthy human keratinocytes and T-cells promotes keratinocyte chemokine production and RORγt-positive IL-17 producing T-cell populations. J Dermatol Sci. 2013;69:44-53 pubmed publisher
Radulovic K, Manta C, Rossini V, Holzmann K, Kestler H, Wegenka U, et al. CD69 regulates type I IFN-induced tolerogenic signals to mucosal CD4 T cells that attenuate their colitogenic potential. J Immunol. 2012;188:2001-13 pubmed publisher
LaCasse C, Janikashvili N, Larmonier C, Alizadeh D, Hanke N, Kartchner J, et al. Th-1 lymphocytes induce dendritic cell tumor killing activity by an IFN-γ-dependent mechanism. J Immunol. 2011;187:6310-7 pubmed publisher
Purvis H, Stoop J, Mann J, Woods S, Kozijn A, Hambleton S, et al. Low-strength T-cell activation promotes Th17 responses. Blood. 2010;116:4829-37 pubmed publisher
Moens E, Brouwer M, Dimova T, Goldman M, Willems F, Vermijlen D. IL-23R and TCR signaling drives the generation of neonatal Vgamma9Vdelta2 T cells expressing high levels of cytotoxic mediators and producing IFN-gamma and IL-17. J Leukoc Biol. 2011;89:743-52 pubmed publisher
Jurado J, Pasquinelli V, Alvarez I, Peña D, Rovetta A, Tateosian N, et al. IL-17 and IFN-γ expression in lymphocytes from patients with active tuberculosis correlates with the severity of the disease. J Leukoc Biol. 2012;91:991-1002 pubmed publisher
Satoh-Takayama N, Lesjean-Pottier S, Vieira P, Sawa S, Eberl G, Vosshenrich C, et al. IL-7 and IL-15 independently program the differentiation of intestinal CD3-NKp46+ cell subsets from Id2-dependent precursors. J Exp Med. 2010;207:273-80 pubmed publisher
Centuori S, Trad M, LaCasse C, Alizadeh D, Larmonier C, Hanke N, et al. Myeloid-derived suppressor cells from tumor-bearing mice impair TGF-β-induced differentiation of CD4+CD25+FoxP3+ Tregs from CD4+CD25-FoxP3- T cells. J Leukoc Biol. 2012;92:987-97 pubmed publisher
Rubinstein M, Cloud C, Garrett T, Moore C, Schwartz K, Johnson C, et al. Ex vivo interleukin-12-priming during CD8(+) T cell activation dramatically improves adoptive T cell transfer antitumor efficacy in a lymphodepleted host. J Am Coll Surg. 2012;214:700-7; discussion 707-8 pubmed publisher
Kebir H, Ifergan I, Alvarez J, Bernard M, Poirier J, Arbour N, et al. Preferential recruitment of interferon-gamma-expressing TH17 cells in multiple sclerosis. Ann Neurol. 2009;66:390-402 pubmed publisher
Gaddis D, Michalek S, Katz J. TLR4 signaling via MyD88 and TRIF differentially shape the CD4+ T cell response to Porphyromonas gingivalis hemagglutinin B. J Immunol. 2011;186:5772-83 pubmed publisher
Sciumè G, Hirahara K, Takahashi H, Laurence A, Villarino A, Singleton K, et al. Distinct requirements for T-bet in gut innate lymphoid cells. J Exp Med. 2012;209:2331-8 pubmed publisher
Ma J, Wang R, Fang X, Ding Y, Sun Z. Critical role of TCF-1 in repression of the IL-17 gene. PLoS ONE. 2011;6:e24768 pubmed publisher
Svobodova E, Krulova M, Zajicova A, Pokorna K, Prochazkova J, Trosan P, et al. The role of mouse mesenchymal stem cells in differentiation of naive T-cells into anti-inflammatory regulatory T-cell or proinflammatory helper T-cell 17 population. Stem Cells Dev. 2012;21:901-10 pubmed publisher
Prochazkova J, Pokorna K, Holan V. IL-12 inhibits the TGF-β-dependent T cell developmental programs and skews the TGF-β-induced differentiation into a Th1-like direction. Immunobiology. 2012;217:74-82 pubmed publisher
Lugering A, Ross M, Sieker M, Heidemann J, Williams I, Domschke W, et al. CCR6 identifies lymphoid tissue inducer cells within cryptopatches. Clin Exp Immunol. 2010;160:440-9 pubmed publisher
Larochelle C, Cayrol R, Kebir H, Alvarez J, Lécuyer M, Ifergan I, et al. Melanoma cell adhesion molecule identifies encephalitogenic T lymphocytes and promotes their recruitment to the central nervous system. Brain. 2012;135:2906-24 pubmed publisher
Poffenberger M, Straka N, El Warry N, Fang D, Shanina I, Horwitz M. Lack of IL-6 during coxsackievirus infection heightens the early immune response resulting in increased severity of chronic autoimmune myocarditis. PLoS ONE. 2009;4:e6207 pubmed publisher
Nakatsukasa H, Tsukimoto M, Tokunaga A, Kojima S. Repeated gamma irradiation attenuates collagen-induced arthritis via up-regulation of regulatory T cells but not by damaging lymphocytes directly. Radiat Res. 2010;174:313-24 pubmed publisher
Fujimoto K, Karuppuchamy T, Takemura N, Shimohigoshi M, Machida T, Haseda Y, et al. A new subset of CD103+CD8alpha+ dendritic cells in the small intestine expresses TLR3, TLR7, and TLR9 and induces Th1 response and CTL activity. J Immunol. 2011;186:6287-95 pubmed publisher
Lu Y, Malmhäll C, Sjöstrand M, Radinger M, O'Neil S, Lotvall J, et al. Expansion of CD4(+) CD25(+) and CD25(-) T-Bet, GATA-3, Foxp3 and RORγt cells in allergic inflammation, local lung distribution and chemokine gene expression. PLoS ONE. 2011;6:e19889 pubmed publisher
Zielinski C, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, et al. Pathogen-induced human TH17 cells produce IFN-γ or IL-10 and are regulated by IL-1β. Nature. 2012;484:514-8 pubmed publisher
Satoh-Takayama N, Dumoutier L, Lesjean-Pottier S, Ribeiro V, Mandelboim O, Renauld J, et al. The natural cytotoxicity receptor NKp46 is dispensable for IL-22-mediated innate intestinal immune defense against Citrobacter rodentium. J Immunol. 2009;183:6579-87 pubmed publisher
Patakas A, Benson R, Withers D, Conigliaro P, McInnes I, Brewer J, et al. Th17 effector cells support B cell responses outside of germinal centres. PLoS ONE. 2012;7:e49715 pubmed publisher
Sestero C, McGuire D, De Sarno P, Brantley E, Soldevila G, Axtell R, et al. CD5-dependent CK2 activation pathway regulates threshold for T cell anergy. J Immunol. 2012;189:2918-30 pubmed publisher
Mjösberg J, Berg G, Jenmalm M, Ernerudh J. FOXP3+ regulatory T cells and T helper 1, T helper 2, and T helper 17 cells in human early pregnancy decidua. Biol Reprod. 2010;82:698-705 pubmed publisher
McGovern J, Nguyen D, Notley C, Mauri C, Isenberg D, Ehrenstein M. Th17 cells are restrained by Treg cells via the inhibition of interleukin-6 in patients with rheumatoid arthritis responding to anti-tumor necrosis factor antibody therapy. Arthritis Rheum. 2012;64:3129-38 pubmed publisher
Lexberg M, Taubner A, Albrecht I, Lepenies I, Richter A, Kamradt T, et al. IFN-γ and IL-12 synergize to convert in vivo generated Th17 into Th1/Th17 cells. Eur J Immunol. 2010;40:3017-27 pubmed publisher
Henry T, Kirimanjeswara G, Ruby T, Jones J, Peng K, Perret M, et al. Type I IFN signaling constrains IL-17A/F secretion by gammadelta T cells during bacterial infections. J Immunol. 2010;184:3755-67 pubmed publisher
Hidalgo L, Martínez V, Valencia J, Hernández-López C, Vázquez M, Núñez J, et al. Expression of BMPRIA on human thymic NK cell precursors: role of BMP signaling in intrathymic NK cell development. Blood. 2012;119:1861-71 pubmed publisher
Kim S, Han S, Withers D, Gaspal F, Bae J, Baik S, et al. CD117⁺ CD3⁻ CD56⁻ OX40Lhigh cells express IL-22 and display an LTi phenotype in human secondary lymphoid tissues. Eur J Immunol. 2011;41:1563-72 pubmed publisher
Sanos S, Diefenbach A. Isolation of NK cells and NK-like cells from the intestinal lamina propria. Methods Mol Biol. 2010;612:505-17 pubmed publisher
Ness-Schwickerath K, Jin C, Morita C. Cytokine requirements for the differentiation and expansion of IL-17A- and IL-22-producing human Vgamma2Vdelta2 T cells. J Immunol. 2010;184:7268-80 pubmed publisher
Li L, Kim J, Boussiotis V. IL-1β-mediated signals preferentially drive conversion of regulatory T cells but not conventional T cells into IL-17-producing cells. J Immunol. 2010;185:4148-53 pubmed publisher
Russell S, Moore A, Fallon P, Walsh P. Soluble IL-2Rα (sCD25) exacerbates autoimmunity and enhances the development of Th17 responses in mice. PLoS ONE. 2012;7:e47748 pubmed publisher
product information
Catalog Number :
14-6988-80
List Price :
139.00 USD
Size :
25 ug
Presku :
14-6988
Product Type :
Antibody
Product Description :
Anti-Human/Mouse ROR gamma (t) Purified 25 ug
Clone :
AFKJS-9
Host :
Rat
Isotype :
IgG2a
Also Known As :
Retinoid-Related Orphan Receptor gamma
Reactivity/Species :
Human , Mouse
Applications :
Immunoprecipitation , Western Blotting
Regulatory Status :
RUO
Conjugate :
Purified
Long Description :
The AJKJS-9 monoclonal antibody reacts with the mouse ROR (t) protein. ROR is a member of the retinoic acid-related orphan receptor (ROR) family, which also includes ROR and ROR . ROR family proteins are ligand-dependent transcription factors that play roles in multiple physiological processes. ROR is expressed in several tissues including liver, lung, muscle, heart and kidney. Furthermore, it was discovered that alternative transcription results in the expression of an isoform, ROR t, which is expressed exclusively in cells of the lymphoid compartment, namely CD4+CD8+ Òdouble-positiveÓ thymoyctes, Th17 cells of the periphery and lymphoid tissue inducer (Lti) cells of lymphoid organs. The ROR t isoform differs from ROR by three unique amino acids at its amino terminus. Therefore, the AFKJS-9 antibody will react with both the ROR and ROR t isoforms.
Parent application :
IP , WB
antigen :
ROR gamma (t)
more info or order :
company information
eBioscience
10255 Science Center Drive
San Diego, CA 92121
info@ebioscience.com
www.ebioscience.com
888.999.1371
headquarters: USA