product summary
request information :
company name :
US Biological
product type :
antibody
product name :
Estrogen Receptor alpha (ERa)
catalog :
E3565-09D
quantity :
50 ug
price :
652.68 USD
clonality :
monoclonal
host :
mouse
more info or order :
product information
Catalog Number :
E3565-09D
Product wo Prefix :
Estrogen Receptor alpha (ERa)
Host :
mouse
Product Type :
Mab
Category :
Antibodies
Size1 :
50 ug
Size1 List :
652.68 USD
Clone # USB :
6D377
Isotype :
IgG1
Desc1 :
Applications: Suitable for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot and Gel Supershift Assays. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Frozen sections; paraffin sections): 5ug/ml. Staining of ER-alpha in mouse Leydig cells results in both nuclear and cytoplasmic staining. Immunocytochemistry: 10ug/ml. Western Blot: 1ug/ml Optimal conditions must be determined individually for each application. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Calc Crossreactivity :
Bo Hu Mk Mo Rt
Immunogen :
Synthetic peptide corresponding to aa 247-261 (E247VGMMKGGIRKDRRG261) of the DNA binding domain of ER-alpha (estrogen receptor-alpha).
Specificity :
Recognizes both the steroid occupied and unoccupied forms of human ER-alpha. Detects a band of ~67kDa by Western blot. Does not cross-react with androgen receptor, PR (progesterone receptor), GR (glucocorticoid receptor) or ER-beta. Does not inhibit binding of ER to nuclei and DNA. Species Crossreactivity: Mouse, rat, bovine and monkey. Species sequence homology: Porcine, sheep, chicken and frog.
Purity :
Ascites
Form :
Supplied as a liquid in PBS containing 0.1% sodium azide.
Concentration :
0.5mg/ml
Desc2 :
Product Type: Mab Isotype: IgG1 Clone No: 6D377 Host: mouse Source: human Concentration: 0.5mg/ml Form: Supplied as a liquid in PBS containing 0.1% sodium azide. Purity: Ascites Immunogen: Synthetic peptide corresponding to aa 247-261 (E247VGMMKGGIRKDRRG261) of the DNA binding domain of ER-alpha (estrogen receptor-alpha). Specificity: Recognizes both the steroid occupied and unoccupied forms of human ER-alpha. Detects a band of ~67kDa by Western blot. Does not cross-react with androgen receptor, PR (progesterone receptor), GR (glucocorticoid receptor) or ER-beta. Does not inhibit binding of ER to nuclei and DNA. Species Crossreactivity: Mouse, rat, bovine and monkey. Species sequence homology: Porcine, sheep, chicken and frog. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
FC D IC IHC IP WB
Storage Temperature :
4°C (-20°C Glycerol)
Reference :
1. A. Traish, et al., (1990) Steroids 55:196. 2. E. Hosli & L. Hosli, (1999) Int. J. Dev. Neurosci. 17:317. 3. S. Cao, et al., (2000) Cytometry 41:109. 4. E. Hosli, et al., (2000) Int. J. Dev. Neurosci. 18:101.
more info or order :
company information
US Biological
4 Technology Way
Salem, MA01970
service@usbio.net
www.usbio.net
800-520-3011
headquarters: USA
United States Biological is a primary brand for the Life Science Industry. We are committed to reducing the cost of research with value, integrity and a truly personal buying experience.