Cdk4 Monoclonal Antibody (DCS-31) | Invitrogen MA5-13498 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Cdk4 Monoclonal Antibody (DCS-31)

catalog :

MA5-13498

quantity :

500 µL

price :

US 436

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DCS-31

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunoprecipitation, flow cytometry

citations: 21

Reference
SaÃ¢da Bouzid E, Burel Vandenbos F, RanchÃ¨re Vince D, Birtwisle Peyrottes I, Chetaille B, Bouvier C, et al. Prognostic value of HMGA2, CDK4, and JUN amplification in well-differentiated and dedifferentiated liposarcomas. Mod Pathol. 2015;28:1404-14 pubmed publisher
Weingertner N, Neuville A, Chibon F, Ray Coquard I, Marcellin L, Ghnassia J. Myxoid liposarcoma with heterologous components: dedifferentiation or metaplasia? A FISH-documented and CGH-documented case report. Appl Immunohistochem Mol Morphol. 2015;23:230-5 pubmed publisher
Val Bernal J, Azueta A, Ortiz Rivas L, Fuentes J, Ballestero R. Incidental lipoma-like hibernoma arising from the adrenal gland: a well-differentiated liposarcoma mimicker. Pathol Res Pract. 2013;209:812-6 pubmed publisher
Lee P, Yau D, Lau P, Chan J. Plexiform fibromyxoma (plexiform angiomyxoid myofibroblastic tumor) of stomach: an unusual presentation as a fistulating abscess. Int J Surg Pathol. 2014;22:286-90 pubmed publisher
Naoe H, Chiyoda T, Ishizawa J, Masuda K, Saya H, Kuninaka S. The APC/C activator Cdh1 regulates the G2/M transition during differentiation of placental trophoblast stem cells. Biochem Biophys Res Commun. 2013;430:757-62 pubmed publisher
Sioletic S, Dal Cin P, Fletcher C, Hornick J. Well-differentiated and dedifferentiated liposarcomas with prominent myxoid stroma: analysis of 56 cases. Histopathology. 2013;62:287-93 pubmed publisher
Kashima T, Halai D, Ye H, Hing S, Delaney D, Pollock R, et al. Sensitivity of MDM2 amplification and unexpected multiple faint alphoid 12 (alpha 12 satellite sequences) signals in atypical lipomatous tumor. Mod Pathol. 2012;25:1384-96 pubmed publisher
Yoshida A, Ushiku T, Motoi T, Beppu Y, Fukayama M, Tsuda H, et al. MDM2 and CDK4 immunohistochemical coexpression in high-grade osteosarcoma: correlation with a dedifferentiated subtype. Am J Surg Pathol. 2012;36:423-31 pubmed publisher
Lee J, Fletcher C. Malignant fat-forming solitary fibrous tumor (so-called "lipomatous hemangiopericytoma"): clinicopathologic analysis of 14 cases. Am J Surg Pathol. 2011;35:1177-85 pubmed publisher
Dujardin F, Binh M, Bouvier C, Gomez Brouchet A, Larousserie F, Muret A, et al. MDM2 and CDK4 immunohistochemistry is a valuable tool in the differential diagnosis of low-grade osteosarcomas and other primary fibro-osseous lesions of the bone. Mod Pathol. 2011;24:624-37 pubmed publisher
Rocha A, Paternot S, Coulonval K, Dumont J, Soares P, Roger P. Cyclic AMP inhibits the proliferation of thyroid carcinoma cell lines through regulation of CDK4 phosphorylation. Mol Biol Cell. 2008;19:4814-25 pubmed publisher
James M, Ray A, Leznova D, Blain S. Differential modification of p27Kip1 controls its cyclin D-cdk4 inhibitory activity. Mol Cell Biol. 2008;28:498-510 pubmed
Landis M, Brown N, Baker G, Shifrin A, Das M, Geng Y, et al. The LxCxE pRb interaction domain of cyclin D1 is dispensable for murine development. Cancer Res. 2007;67:7613-20 pubmed
Yu Q, Sicinska E, Geng Y, Ahnström M, Zagozdzon A, Kong Y, et al. Requirement for CDK4 kinase function in breast cancer. Cancer Cell. 2006;9:23-32 pubmed
Raynaud F, Whittaker S, Fischer P, McClue S, Walton M, Barrie S, et al. In vitro and in vivo pharmacokinetic-pharmacodynamic relationships for the trisubstituted aminopurine cyclin-dependent kinase inhibitors olomoucine, bohemine and CYC202. Clin Cancer Res. 2005;11:4875-87 pubmed
Rodier G, Makris C, Coulombe P, Scime A, Nakayama K, Nakayama K, et al. p107 inhibits G1 to S phase progression by down-regulating expression of the F-box protein Skp2. J Cell Biol. 2005;168:55-66 pubmed
Coindre J, Hostein I, Maire G, Derré J, Guillou L, Leroux A, et al. Inflammatory malignant fibrous histiocytomas and dedifferentiated liposarcomas: histological review, genomic profile, and MDM2 and CDK4 status favour a single entity. J Pathol. 2004;203:822-30 pubmed
Ilyin G, Glaise D, Gilot D, Baffet G, Guguen Guillouzo C. Regulation and role of p21 and p27 cyclin-dependent kinase inhibitors during hepatocyte differentiation and growth. Am J Physiol Gastrointest Liver Physiol. 2003;285:G115-27 pubmed
Coindre J, Mariani O, Chibon F, Mairal A, De Saint Aubain Somerhausen N, Favre Guillevin E, et al. Most malignant fibrous histiocytomas developed in the retroperitoneum are dedifferentiated liposarcomas: a review of 25 cases initially diagnosed as malignant fibrous histiocytoma. Mod Pathol. 2003;16:256-62 pubmed
Lavictoire S, Parolin D, Klimowicz A, Kelly J, Lorimer I. Interaction of Hsp90 with the nascent form of the mutant epidermal growth factor receptor EGFRvIII. J Biol Chem. 2003;278:5292-9 pubmed
Tsutsui T, Hesabi B, Moons D, Pandolfi P, Hansel K, Koff A, et al. Targeted disruption of CDK4 delays cell cycle entry with enhanced p27(Kip1) activity. Mol Cell Biol. 1999;19:7011-9 pubmed

product information

Product Type :

Antibody

Product Name :

Cdk4 Monoclonal Antibody (DCS-31)

Catalog # :

MA5-13498

Quantity :

500 µL

Price :

US 436

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

Flow Cytometry: 0.5 µg/test, Immunofluorescence: Assay Dependent, Immunoprecipitation: 2 µg/mg protein lysate, Western Blot: 1-2 µg/mL

Species :

Human, Mouse, Rat

Clone :

DCS-31

Isotype :

IgG1

Storage :

4° C

Description :

CDK4 is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of the gene have been reported.

Immunogen :

Recombinant cdk4 protein

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 0.5 µg/test, Immunofluorescence: Assay Dependent, Immunoprecipitation: 2 µg/mg protein lysate, Western Blot: 1-2 µg/mL

Aliases :

Cdk4; Cell division protein kinase 4; CMM3; Crk3; cyclin dependent kinase 4; cyclin-dependent kinase 4; MGC14458; p34. /cdk4; PSK-J3; serine/threonine kinase; Unknown (protein for MGC:133903)