HIF-1 Antibody (OZ12) | Invitrogen MA1-12612 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon

product type :

antibody

product name :

HIF-1 Antibody (OZ12)

catalog :

MA1-12612

quantity :

100µg

price :

US 455.00

clonality :

monoclonal

host :

mouse

reactivity :

human, rat

application :

immunohistochemistry, immunocytochemistry, immunoprecipitation, EMSA

citations: 7

	Bendinelli P, Maroni P, Matteucci E, Luzzati A, Perrucchini G, Desiderio M. Hypoxia inducible factor-1 is activated by transcriptional co-activator with PDZ-binding motif (TAZ) versus WWdomain-containing oxidoreductase (WWOX) in hypoxic microenvironment of bone metastasis from breast cancer. Eur J Cancer. 2013;49:2608-18 pubmed publisher
	Sanchez-Elsner T, Botella L, Velasco B, Langa C, Bernabeu C. Endoglin expression is regulated by transcriptional cooperation between the hypoxia and transforming growth factor-beta pathways. J Biol Chem. 2002;277:43799-808 pubmed
	Salnikow K, An W, Melillo G, Blagosklonny M, Costa M. Nickel-induced transformation shifts the balance between HIF-1 and p53 transcription factors. Carcinogenesis. 1999;20:1819-23 pubmed
	Sobajima J, Kumamoto K, Haga N, Tamaru J, Takahashi T, Miyazaki T, et al. Early evaluation of the apoptotic index ratio is useful in predicting the efficacy of chemoradiotherapy in esophageal squamous cell carcinoma. Oncol Lett. 2012;3:287-292 pubmed
	Sohda M, Ishikawa H, Masuda N, Kato H, Miyazaki T, Nakajima M, et al. Pretreatment evaluation of combined HIF-1alpha, p53 and p21 expression is a useful and sensitive indicator of response to radiation and chemotherapy in esophageal cancer. Int J Cancer. 2004;110:838-44 pubmed
	Giannopoulou E, Ravazoula P, Kalofonos H, Makatsoris T, Kardamakis D. Expression of HIF-1alpha and iNOS in astrocytic gliomas: a clinicopathological study. In Vivo. 2006;20:421-5 pubmed
	Del Bigio M, Khan O, da Silva Lopes L, Juliet P. Cerebral white matter oxidation and nitrosylation in young rodents with kaolin-induced hydrocephalus. J Neuropathol Exp Neurol. 2012;71:274-88 pubmed publisher

product information

Product Type :

Antibody

Product Name :

HIF-1 Antibody (OZ12)

Catalog # :

MA1-12612

Quantity :

100µg

Price :

US 455.00

Clonality :

Monoclonal

Purity :

Protein A/G

Host :

Mouse

Reactivity :

Applications :

Immunofluorescence: Assay Dependent, Immunoprecipitation: 2 µg/mg lysate, Gel Shift: 1 mg/ml

Species :

Clone :

OZ12

Isotype :

IgG1

Storage :

-20¡ C, Avoid Freeze/Thaw Cycles

Description :

Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets.

Immunogen :

Human HIF-1 (residues 530-826) protein.

Format :

Liquid

Applications w/Dilutions :

IF Assay Dependent, IP 2 µg/mg lysate, GS 1 mg/ml

Aliases :

Hypoxia inducing factor 1

PubMed References :

10469629, 12228247, 15170665