This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Rockland Immunochemicals
product type :
other
product name :
GAPDH Antibody
catalog :
600-401-A33S
quantity :
25 µL
price :
103.00 USD
product information
Catalog Number :
600-401-A33S
Name :
Anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (RABBIT) Antibody - 600-401-A33S
Display Name :
GAPDH Antibody
Application Note :
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation.  Expect a band at ~39 kDa in size corresponding to GAPDH by western blotting in the appropriate cell lysate or extract.  GAPDH is constitutively expressed at high levels in almost all tissues and cell lines, making it ideal for use as a loading control marker.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
1.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
25 µL
Default Unit :
ul
ELISA Dilution :
1:10,000 - 1:40,000
Immunohistochemistry Dilution :
1:100 - 1:1,000
IF Microscopy Dilution :
1:100 - 1:1,000
Western Blot Dilution :
1:1,000 - 1:5,000
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG
Gene Name :
GAPDH
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH).
Packing Type :
Wet Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
GAPDH antibody is directed against human GAPDH protein.  The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.  A BLAST analysis was used to suggest that this antibody would react with GAPDH from a wide range of organisms, including most vertebrates and some yeast.  Broad reactivity makes this antibody an excellent loading control.
Species Reactivity :
Mouse, Human, Rat, Rabbit, Chicken, Bovine, Swine
Storage :
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Synonyms :
Loading Control Antibody, Anti-GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD
Target Species :
Human
Background :
Anti-GAPDH Western Blot loading control antibody is an ideal control for your westerns. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD).  This is an important energy-yielding step in carbohydrate metabolism.  Recent evidence suggests that GAPDH is also involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export.  GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease.  GAPDH is constitutively expressed at high levels in almost all tissues and cell lines, making it ideal for use as a loading control marker in immunoblots.
General Reference :
Sirover,M.A. (2005) New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells. J. Cell. Biochem. 95:45-52. Glyceraldehyde-3-phosphate dehydrogenase, apoptosis, and neurodegenerative diseases. Annu. Rev. Pharmacol. Toxicol. (2005); 45:269-290. Raje,C.I., Kumar,S., Harle,A., Nanda,J.S. and Raje,M. (2007) The macrophage cell surface glyceraldehyde-3-phosphate dehydrogenase is a novel transferrin receptor. J. Biol. Chem. 282 (5):3252-3261.
Immunogen Type :
Peptide
Low Endotoxin :
No
Sample Size :
Yes
Target Domain of Reactivity :
N-Terminus
Created On :
12/15/2011 7:17 PM
Modified On :
9/18/2017 2:59 PM
Application Text :
ELISA,Immunohistochemistry,IF Microscopy,Western Blot,
Approved for General Distributors :
12/1/2014
Discontinued Item :
Yes
Division :
OEM
Launch Date :
10/26/2011
Other :
User Optimized
Vendor :
ProSci
Status :
Active
Category :
Controls
Conjugation Name :
Unconjugated
2018 Price :
103.00 USD
UniProt :
P04406
NCBI :
7669492
Primary Image Name :
Anti-GAPDH Antibody - Immunohistochemistry
company information
Rockland Immunochemicals
P.O. Box 326
Gilbertsville, PA 19525
Info@rockland-inc.com
www.rockland-inc.com
1-610-369-1008
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.