antibody

DELTA-4 Antibody

600-401-696S

25 µL

99.00 USD

polyclonal

rabbit

nonconjugated

western blot, ELISA, immunohistochemistry

Anti-DELTA-4 (RABBIT) Antibody - 600-401-696S

DELTA-4 Antibody

Anti-DELTA-4 has been tested for use in ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 74 kDa in size corresponding to DELTA-4 by western blotting in the appropriate cell lysate or extract. For best results use Goat Serum as blocking agent in western blotting.

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Polyclonal

1.0mg/ml

by UV absorbance at 280 nm

(None)

25 µL

ul

1:5,000 - 1:25,000

10 µg/mL to 20 µg/mL

1:500 - 1:2,500

Expiration date is one (1) year from date of opening.

IgG

DLL4

Rabbit

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Anti-DELTA-4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human DELTA-4.

Dry Ice

Liquid (sterile filtered)

0.01% (w/v) Sodium Azide

Anti-DELTA-4 affinity purified antibody is directed against human DELTA-4. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest reactivity with this protein from human based on 100% homology for the immunogen sequence. However, cross reactivity is expected with mouse, rat and chimpanzee DELTA-4 based on a 91% homology to the core 12 amino acid sequence within the 14 amino acid immunogen sequence. Cross reactivity with DELTA-4 homologues from other sources has not been determined.

human

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

rabbit anti-DELTA4 antibody, DELTA-4, delta 4 precursor, Delta-like protein 4, DLL4, Drosophila Delta homolog 4, notch ligand DLL4 precursor, DELTA 4

Human

DELTA-4 (also called delta 4 precursor, delta ligand 4 precursor, Delta-like protein 4, DLL4, Drosophila Delta homolog 4 and notch ligand DLL4 precursor) is a homolog of the Drosophila delta protein. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. DELTA-4 plays a role in the Notch signaling pathway and activates Notch-1 and Notch-4. DELTA-4 is a membrane protein that is expressed in the vascular endothelium.

Lauret,E., et al. (2004) Membrane-bound delta-4 notch ligand reduces the proliferative activity of primitive human hematopoietic CD34+CD38low cells while maintaining their LTC-IC potential. Leukemia 18 (4), 788-797. Tohda,S., Murata-Ohsawa,M., Sakano,S. and Nara,N. (2003) Notch ligands, Delta-1 and Delta-4 suppress the self-renewal capacity and long-term growth of two myeloblastic leukemia cell lines. Int. J. Oncol. 22 (5), 1073-1079. Liu,Z.J., Shirakawa,T., Li,Y., Soma,A., Oka,M., Dotto,G.P., Fairman,R.M., Velazquez,O.C. and Herlyn,M. (2003) Regulation of Notch1 and Dll4 by vascular endothelial growth factor in arterial endothelial cells: implications for modulating arteriogenesis and angiogenesis. Mol. Cell. Biol. 23 (1), 14-25.

Indraccolo S, Minuzzo S, Masiero M, et al. Cross-talk between tumor and endothelial cells involving the Notch3-Dll4 interaction marks escape from tumor dormancy. Cancer Res. 2009 Feb 15;69(4):1314–1323. Wu S, Esumi S, Watanabe K, et al. Tangential migration and proliferation of intermediate progenitors of GABAergic neurons in the mouse telencephalon. Development. 2011 Jun;138(12):2499–2509. Zhang Z, Zeng B, Zhang Z, et al. Suppressor of cytokine signaling 3 promotes bone marrow cells to differentiate into CD8+ T lymphocytes in lung tissue via up-regulating Notch1 expression. Cancer Res. 2009 Feb 15;69(4):1578–1586. Mitsuhashi Y, Horiuchi A, Miyamoto T, Kashima H, Suzuki A, Shiozawa T. (2012) Prognostic significance of Notch signalling molecules and their involvement in the invasiveness of endometrial carcinoma cells. Histopathology. 2012 Apr;60(5):826-37. doi: 10.1111/j.1365-2559.2011.04158.x. Epub 2012 Feb 20.

Peptide

No

Yes

Internal

ELISA,Immunohistochemistry,Western Blot,

User Optimized

Primary Antibodies

Unconjugated

99.00 USD

Q9NR61

9506545

Anti-Delta-4 Antibody - Immunohistochemistry