Mesothelin Antibody | Rockland Immunochemicals 200-301-A88S product information

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product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Mesothelin Antibody

catalog :

200-301-A88S

quantity :

25 µL

price :

99.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MN-1

reactivity :

human

application :

western blot, ELISA, immunohistochemistry

product information

Catalog Number :

200-301-A88S

Name :

Anti-Mesothelin (MOUSE) Monoclonal Antibody - 200-301-A88S

Display Name :

Mesothelin Antibody

Application Note :

This antibody has been tested for use in immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kDa in size corresponding to mature mesothelin by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, archival PEFF human tissues were deparaffinized followed by hydration. Antigen-retrieval is recommended. Block tissues with 1% BSA in PBS for 30 min at 23° C. Antibodies are diluted in 1% BSA and reacted with tissue for 60 min at room temperature.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

MN-1

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

25 µL

Default Unit :

ELISA Dilution :

1:10,000 - 1:50,000

Immunohistochemistry Dilution :

1:100

Western Blot Dilution :

1:1,000

Expiration :

Expiration date is three (3) months from date of opening.

Format :

IgG2a

Gene Name :

MSLN

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This antibody was produced in mesothelin-deficient mice by immunizations with plasmid cDNA encoding human MSLN full length protein followed by a single boost of a recombinant human mesothelin-Fc fusion protein.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This antibody is directed against human mesothelin protein. This product was purified from tissue culture supernatant fluid by Protein A chromatography. Cross reactivity with homologues from other sources has not been tested.

Species Reactivity :

human

Storage :

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Subclass :

IgG2a

Synonyms :

mouse anti-Mesothelin Antibody, Mesothelian, MN, MB, Pre-pro-megakaryocyte-potentiating factor, CAK1 antigen

Target Species :

Human

Background :

Mesothelin is a glycosyl-phosphatidyl-inositol–anchored glycoprotein present on the cell surface of various human solid tumors. The mesothelin (MSLN) gene encodes a 71-kDa precursor protein that is processed to a 40-kDa glycosylphosphatidyl-inositol–anchored protein that composes the mature portion and an NH2-terminal 31-kDa fragment called megakaryocyte-potentiating factor that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues, such as mesothelium, but aberrantly over expressed in mesotheliomas, ovarian, and pancreatic cancers. The biological functions of mesothelin remain elusive. A recent study showed that mesothelin binds to MUC16/CA125, and that this interaction mediates cell adhesion, suggesting that there may be an important role for MUC16/CA125 and mesothelin in the metastatic spread of ovarian cancer.

General Reference :

Chang K, Pastan I, Willingham MC. (1992) Isolation and characterization of a monoclonal antibody reactive with ovarian cancers and normal mesothelium. Int J Cancer 50:373–81. Argani P, Iacobuzio-Danahue C, Ryu B, et al. (2001) Mesothelin is overexpressed in the vast majority of ductal adenocarcinomas of the pancreas: identification of a new pancreatic cancer marker by serial analysis of gene expression (SAGE). Clin Cancer Res 7:3862–8. Chang K, Pastan I.(1996) Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesothelioma, and ovarian cancers. Proc Natl Acad Sci U S A 93:136–40.

Specific Reference :

Onda, M., Willingham, M., Nagata, S., Bera, T.K., Beers, R., Ho, M., Hassan, R., Kreitman, R.J., Pastan, I. (2005) New monoclonal antibodies to mesothelin useful for immunohistochemistry, fluorescence-activated cell sorting, Western blotting, and ELISA. Clin Cancer Res. 11(16):5840-6. Ho, M., Bera, T.K., Willingham, M., Onda, M., Hassan, R., Fitzgerald, D. and Pastan, I. (2007) Mesothelin Expression in Human Lung Cancer. Clin Cancer Res 13:1571-5. Feng, M., Zhang, J., Anver, M., Hassan, R., & Ho, M. (2011). In vivo imaging of human malignant mesothelioma grown orthotopically in the peritoneal cavity of nude mice. Journal of Cancer, 2, 123. O’Shannessy, D. J., Somers, E. B., Palmer, L. M., Thiel, R. P., Oberoi, P., Heath, R., & Marcucci, L. (2013). Serum folate receptor alpha, mesothelin and megakaryocyte potentiating factor in ovarian cancer: association to disease stage and grade and comparison to CA125 and HE4. J Ovarian Res, 6(1), 29.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Yes

Target Domain of Reactivity :

Extracellular

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2019 Price :

99.00 USD

UniProt :

Q13421

NCBI :

53988378

Primary Image Name :

Anti-Mesothelin Antibodies - Immunohistochemistry