Novus Biologicals

IMGENEX

antibody

Mouse Monoclonal Insulin R/CD220 Antibody

NB400-142

0.1 ml

329 USD

monoclonal

mouse

mouse, rat, human

immunoprecipitation, ELISA, neutralization, western blot

NB400-142

Mouse Monoclonal Insulin R/CD220 Antibody

329 USD

Primary Antibodies

0.1 ml

CD 220, CD220, CD220 antigen, EC 2.7.10, EC 2.7.10.1, HHF5, insulin receptor, IR

10 mM phosphate buffer [pH 7.4], 100 mM NaCl.

Monoclonal

MA-20

1.2 mg/ml

Western Blot, Blocking/Neutralizing, ELISA, Immunoprecipitation

Mouse

Highly purified Insulin Receptor (alpha-subunit) from human term placentas. [UniProt# P06213]

IgG2b

Membrane; single pass type I membrane protein.

0.1% Sodium Azide

Protein G purified

Human and rat. Mouse reactivity reported in scientific literature (PMID: 24389353)

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

P06213

This Insulin Receptor (MA-20) antibody is useful for ELISA, Immunoprecipitation, Western blot and Blocking/Neutralizing assays.

Diabetes Research, Lipid and Metabolism, Neuroscience, Nutrient Sensing in the Brain, Protein Kinase, Signal Transduction, Tyrosine Kinases

Morcavallo A, Buraschi S, Xu SQ et al. Decorin differentially modulates the activity of insulin receptor isoform A ligands. Matrix Biol 2014 Jan 2 [PMID: 24389353] (ELISA, Mouse) Vella V, Pandini G, Sciacca L, Mineo R, Vigneri R, Pezzino V, Belfiore A. A novel autocrine loop involving IGF-II and the insulin receptor isoform-A stimulates growth of thyroid cancer. J Clin Endocrinol Metab;87(1):245-54. 2002 Jan. [PMID: 11788654] Li, M et al. Small molecule insulin receptor activators potentiate insulin action in insulin-resistant cells. Diabetes;50(10):2323-8. 2001 Oct. [PMID: 11574415] (WB, ELISA, Human) Costanzo BV, Trischitta V, Di Paola R, Spampinato D, Pizzuti A, Vigneri R, Frittitta L. The Q allele variant (GLN121) of membrane glycoprotein PC-1 interacts with the insulin receptor and inhibits insulin signaling more effectively than the common K allele variant (LYS121). Diabetes;50(4):831-6. 2001 Apr. [PMID: 11289049] (ELISA, Human) Maddux BA, Goldfine ID. Membrane glycoprotein PC-1 inhibition of insulin receptor function occurs via direct interaction with the receptor alpha-subunit. Diabetes;49(1):13-9. 2000 Jan. [PMID: 10615944] Pandini G, Vigneri R, Costantino A, Frasca F, Ippolito A, Fujita-Yamaguchi Y, Siddle K, Goldfine ID, Belfiore A. Insulin and insulin-like growth factor-I (IGF-I) receptor overexpression in breast cancers leads to insulin/IGF-I hybrid receptor overexpression: evidence for a second mechanism of IGF-I signaling. Clin Cancer Res;5(7):1935-44. 1999 Jul. [PMID: 10430101] Frasca, F et al. Insulin receptor isoform A, a newly recognized, high-affinity insulin-like growth factor II receptor in fetal and cancer cells. Mol Cell Biol;19(5):3278-88. 1999 May. [PMID: 10207053] (IP, ELISA, Human) Forsayeth JR, Caro JF, Sinha MK, Maddux BA, Goldfine ID. Monoclonal antibodies to the human insulin receptor that activate glucose transport but not insulin receptor kinase activity. Proc Natl Acad Sci U S A;84(10):3448-51. 1987 May. [PMID: 3033648] (IP, B/N, Human) Pandini G, Frasca F, Mineo R, Sciacca L, Vigneri R, Belfiore A. Insulin/insulin-like growth factor I hybrid receptors have different biological characteristics depending on the insulin receptor isoform involved. J Biol Chem;277(42):39684-95. 2002 Oct 18. [PMID: 12138094] (WB, IP, ELISA , Human) Federici, M et al. Increased expression of insulin/insulin-like growth factor-I hybrid receptors in skeletal muscle of noninsulin-dependent diabetes mellitus subjects. J Clin Invest;98(12):2887-93. 1996 Dec 15. [PMID: 8981937] (WB, Human) Gherzi R, Russell DS, Taylor SI, Rosen OM. Reevaluation of the evidence that an antibody to the insulin receptor is insulinmimetic without activating the protein tyrosine kinase activity of the receptor. J Biol Chem;262(35):16900-5. 1987 Dec 15. [PMID: 3680277] Hawley DM, Maddux BA, Patel RG, Wong KY, Mamula PW, Firestone GL, Brunetti A, Verspohl E, Goldfine ID. Insulin receptor monoclonal antibodies that mimic insulin action without activating tyrosine kinase. J Biol Chem;264(5):2438-44. 1989 Feb 15. [PMID: 2536710] Forsayeth, JR et al. Effect of monoclonal antibodies on human insulin receptor autophosphorylation, negative cooperativity, and down-regulation. J Biol Chem;262(9):4134-40. 1987 Mar 25. [PMID: 2435731] (B/N, Human) Milazzo G, Giorgino F, Damante G, Sung C, Stampfer MR, Vigneri R, Goldfine ID, Belfiore A. Insulin receptor expression and function in human breast cancer cell lines. Cancer Res;52(14):3924-30. 1992 Jul 15. [PMID: 1617668]