product summary
request information :
company name :
Novus Biologicals
product type :
antibody
product name :
Rabbit Polyclonal GAPDH Antibody
catalog :
NB300-324C
quantity :
0.1 ml
price :
315.00 USD
clonality :
polyclonal
host :
rabbit
reactivity :
human
application :
western blot
more info or order :
Novus Biologicals product webpage
product information
Catalog Number :
NB300-324C
Product Name :
Rabbit Polyclonal GAPDH Antibody
List Price :
315.00 USD
Product Type :
Primary Antibodies
Package Size :
0.1 ml
AltNames :
aging-associated gene 9 protein, EC 1.2.1, EC 1.2.1.12, EC 2.6.99.-, G3PD, GAPDPeptidyl-cysteine S-nitrosylase GAPDH, glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, MGC88685
Background :
GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH as a protein expressed in large amounts and which is required at all times for an important house keeping functions, levels of GAPDH mRNA are often used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH as loading controls for western blotting experiments. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations with actin and tubulin have also be reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic.
Buffer :
50mM sodium borate
Clonality :
Polyclonal
Concentration :
0.2 mg/ml
Conjugate :
DyLight 650
Application Summary :
Western Blot
Host :
Rabbit
Immunogen :
The epitope recognized by this antibody maps to a region between residues 250 and 300 of human Glyceraldehyde-3-Phosphate Dehydrogenase using the numbering given in entry NP_002037.2 (GeneID 2597).
Preservative :
No Preservative
Purity :
Immunogen affinity purified
CrossReactivity :
Human and Mouse. Reactivity with X. laevis GAPDH is expected owing to conservation of sequence between this ortholog and Human GAPDH at the site to which the epitope maps.
Storage :
Store at 4C in the dark.
Swissprot Human :
NP_002037.2
Uses :
Prepare working dilution immediately before use. Western Blot
Research Area :
Alzheimers Research, Cancer, Chromatin Research, Lipid and Metabolism, Loading Controls, Neuroscience, Neuronal Cell Markers, Neurodegeneration, Nuclear Receptors Coactivators and Corepressors, Signal Transduction
more info or order :
Novus Biologicals product webpage
company information
Novus Biologicals
8100 Southpark Way, A-8
Littleton, CO 80120
novus@novusbio.com
www.novusbio.com
3037301950
headquarters: usa
The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products in the forefront of science. Novus Biologicals was organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research. All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving niche and emerging markets, Novus Biologicals has become specialists in markets and has formed and will continue to form ongoing partnerships with our customers.