catalog number :
products type :
products full name :
Human choline acetyltransterase, ChAT ELISA Kit
products short name :
products name syn :
Human choline acetyltransterase (ChAT) ELISA Kit; CMS1A; CMS1A2; acetyl CoA:choline O-acetyltransferase; choline acetyltransferase
other names :
choline O-acetyltransferase isoform 1; Choline O-acetyltransferase; choline O-acetyltransferase; choline acetylase; acetyl CoA:choline O-acetyltransferase; choline O-acetyltransferase
products gene name :
other gene names :
CHAT; CHAT; CMS1A; CMS1A2; CHOACTASE; CHOACTase; ChAT; Choline acetylase
uniprot entry name :
This assay has high sensitivity and excellent specificity for detection of human ChAT. No significant cross-reactivity or interference between human ChAT and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: For the quantitative determination of human choline acetyltransterase (ChAT) concentrations in serum, plasma, tissue homogenates, cell lysates. Detection Range: 15.6 pg/ml -1000 pg/ml.
other info2 :
Sensitivity: The minimum detectable dose of human ChAT is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ChAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ChAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ChAT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ChAT bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
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ncbi mol weight :
ncbi pathways :
Acetylcholine Neurotransmitter Release Cycle Pathway 106525!!Acetylcholine Synthesis Pathway 198820!!Biogenic Amine Synthesis Pathway 198793!!Cholinergic Synapse Pathway 217716!!Glycerophospholipid Metabolism Pathway 82989!!Glycerophospholipid Metabolism Pathway 364!!Neuronal System Pathway 106513!!Neurotransmitter Release Cycle Pathway 106518!!Transmission Across Chemical Synapses Pathway 106516
ncbi summary :
This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]
uniprot summary :
Function: Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Catalytic activity: Acetyl-CoA + choline = CoA + O-acetylcholine.