catalog number :
products type :
products full name :
human copeptin ELISA Kit
products short name :
products name syn :
human copeptin ELISA kit; copeptin
other names :
products gene name :
This assay has high sensitivity and excellent specificity for detection of human Copeptin. No significant cross-reactivity or interference between human Copeptin and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: For the quantitative determination of human copeptin concentrations in serum, urine, cell culture supernates, tissue homogenates. Detection Range: 78 pg/ml -5000 pg/ml.
other info2 :
Sensitivity: The minimum detectable dose of human Copeptin is typically less than 19.5 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Copeptin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Copeptin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Copeptin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Copeptin bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi pathways :
Focal Adhesion Pathway 83067!!Focal Adhesion Pathway 478!!MAPK Signaling Pathway 83048!!MAPK Signaling Pathway 456!!Salmonella Infection Pathway 375172!!Salmonella Infection Pathway 375149