antibody

a-Insulin Receptor, beta subunit (INSR, Insulin receptor)

MBS619232

0.1 mL

595 USD

polyclonal

rabbit

nonconjugated

human, mouse

western blot, immunoprecipitation

Antibody

a-Insulin Receptor, beta subunit (INSR, Insulin receptor)

a-Insulin Receptor, beta subunit

Anti -a-Insulin Receptor, beta subunit (INSR, Insulin receptor)

Polyclonal

Rabbit

Human, Mouse, Rat

Recognizes human a-Insulin Receptor, beta subunit. Species Crossreactivity

Affinity Purified. Purified by immunoaffinity chromatography.

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB), Immunoprecipitation (IP)

Suitable for use in Western Blot and Immunoprecipitation. Dilution: Western Blot: 1:1000

Immunogen: GST-fusion protein corresponding to the last 100 C-terminal amino acid residues of the human insulin receptor beta chain.

Positive Control: Human brain tissue lysate

Antibodies; Abs to Receptors

The insulin receptor is a tyrosine kinase receptor that when bound to insulin, initiates multiple signal transduction pathways, including JNK, PI 3-kinase, Akt and PKC. Pharmacological intervention of these Insulin R-dependent pathways is of great interest for the treatment of insulin resistance, obesity and diabetes. The Insulin Receptor (IR) is synthesized as a single polypeptide, which is subsequently cleaved to generate an extracellular alpha-chain and a transmembrane and intracellular beta-chain, tethered together by disulfide bonds. The beta-chain has multiple tyrosine phosphorylation sites, including three autophosphorylation sites at its activation loop. The overall structure of the IR is highly homologous to the IGF-I Receptor, except in their c-termini, where the two proteins diverge somewhat. The IR signals primarily by phosphorylating the Insulin Receptor Substrate (IRS) family of proteins, which creates docking sites for SH2-domain containing proteins. Insulin signaling is highly dependent on the PI3 Kinase pathway and Akt, which appear to mediate the functions of insulin.

0.1 mL

595 USD