antibody

RABBIT ANTI 14-3-3 TAU/THETA

MBS222335

0.1 ml

490 USD

polyclonal

rabbit

nonconjugated

western blot, ELISA, enzyme immunoassay

Antibody

RABBIT ANTI 14-3-3 TAU/THETA

14-3-3 TAU/THETA

14-3-3 TAU/THETA

Polyclonal

IgG

Rabbit

Bovine, Chicken, Human, Mammals (Expected from Sequence), Mouse, Rabbit, Rat

This item recognises 14-3-3 tau/theta in all mammals. It is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple cell signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preferences for it's different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 tau/theta is primarily expressed in T-cells although it occurs at lower levels in the brain, heart, pancreas placenta and kidneys. It plays a role in cell cycle progression via interaction with p27(Kip1). Along with COPI, the theta isoform binds the GB1 RSR sequence involved in protein trafficking. The isoform forms a complex with Hsp60 and cellular prion protein which may be involved in prion diseases. Elevated levels of 14-3-3 tau/theta are observed in amyotrophic lateral sclerosis. 14-3-3 tau/theta also binds BCR/Abl, bacterial effector protein Tir, yes-associated protein and the FSH receptor. This item may not react with recombinant proteins that are not N-acetylated.

Serum. Serum - liquid

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB)

Western Blot: This item detects a band of approximately 30kDa in HEK293 cell lysates. Immunohistology - Paraffin: Maximum Dilution: 1/60; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. . Western Blotting: Maximum Dilution: 1/3000

Perservative Stabilisers: 0.09% Sodium Azide (NaN3)

Immunogen: Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 tau/theta. Peptide sequence: Ac.MEKTELIQKAC. Histology Positive Control Tissue: Normal tonsil tissue. Antiserum Preparation: Antisera to sheep 14-3-3 tau/theta were raised by repeated immunisations of rabbit with highly purified antigen. Target Species: Sheep

Rabbit anti 14-3-3 / antibody recognizes 14-3-3 tau/theta in all mammals. It is a member of the 14-3-3 family which consists of 30 kDa proteins involved in multiple cell signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preferences for it's different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 tau/theta is primarily expressed in T-cells although it occurs at lower levels in the brain, heart, pancreas placenta and kidneys. It plays a role in cell cycle progression via interaction with p27(Kip1). Along with COPI, the theta isoform binds the GB1 RSR sequence involved in protein trafficking. The isoform forms a complex with Hsp60 and cellular prion protein which may be involved in prion diseases. Elevated levels of 14-3-3 tau/theta are observed in amyotrophic lateral sclerosis. 14-3-3 tau/theta also binds BCR/Abl, bacterial effector protein Tir, yes-associated protein and the FSH receptor. Rabbit anti 14-3-3 / antibody may not react with recombinant proteins that are not N-acetylated.

0.1 ml

490 USD