antibody

Anti-Angiopoietin 1 antibody

MBS175341

0.1 mg

245 USD

polyclonal

rabbit

nonconjugated

human, rat

western blot, immunohistochemistry, immunohistochemistry - paraffin section

Antibody

Anti-Angiopoietin 1 antibody

Angiopoietin 1

Angiopoietin-1(ANG-1); angiopoietin 1; AGP 1 antibody; AGP1 antibody; AGPT antibody; ANG 1 antibody; ANG-1 antibody; ANG1 antibody; Angiopoietin-1 antibody; Angiopoietin1 antibody; ANGP1_HUMAN antibody; ANGPT 1 antibody; ANGPT1 antibody; KIAA0003 antibody; OTTHUMP00000227592 antibody; OTTHUMP00000227599 antibody; OTTHUMP00000227600 antibody

Angiopoietin-1; Angiopoietin-1; angiopoietin-1; ANG-1; angiopoietin 1

ANGPT1

ANGPT1; ANGPT1; AGP1; AGPT; ANG1; KIAA0003; ANG-1

ANGP1_HUMAN

Polyclonal

IgG

Rabbit

Reacts with: Human. Predicted to work with: Mouse, rat.

498

No cross reactivity with other proteins.

Immunogen affinity purified.

Lyophilized

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Western Blot (WB):. Concentration: 0.1-0.5ug/ml. Tested Species: Hu. Predicted Species: Ms, Rat. Antigen Retrieval: -. Immunohistochemistry (Paraffin-embedded Section): . Concentration: 0.5-1 ug/ml. Tested Species: Hu. Predicted Species: Ms, Rat. Antigen Retrieval: By Heat. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formailin/ paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Angiopoietin 1 (220-237aa QGLVTRQTYIIQELEKQL), different from the related mouse sequence by two amino acids, and different from the related rat sequence by only one amino acid.

Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Description: Rabbit IgG polyclonal antibody for Angiopoietin-1(ANGPT1) detection. Tested with WB, IHC-P in Human, Mouse, Rat. Background: Angiopoietin 1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Angiopoietin-1 seems to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Endothelial Tie2/Tek ligands angiopoietin-1(ANGPT1) and angiopoietin-2(ANGPT2): regional localization of the human genes to 8q22.3-q23 and 8p23.

12229574

Q15389.2

Q15389

57,456 Da

Angiogenesis Pathway (198772); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Cell Surface Interactions At The Vascular Wall Pathway (106062); HIF-1 Signaling Pathway (695200); Hemostasis Pathway (106028); PI3K-Akt Signaling Pathway (692234); PI3K-Akt Signaling Pathway (692979); Rap1 Signaling Pathway (868086); Rap1 Signaling Pathway (878042); Ras Signaling Pathway (868085)

Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Dec 2010]

ANGPT1: Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 8q23.1. Cellular Component: extracellular space; microvillus; extracellular region; plasma membrane; lipid raft. Molecular Function: receptor tyrosine kinase binding. Biological Process: transmembrane receptor protein tyrosine kinase activation (dimerization); positive regulation of cell adhesion; positive regulation of receptor internalization; negative regulation of protein import into nucleus; negative regulation of cell adhesion; positive chemotaxis; negative regulation of protein amino acid phosphorylation; hemopoiesis; negative regulation of neuron apoptosis; regulation of I-kappaB kinase/NF-kappaB cascade; cell differentiation; Tie receptor signaling pathway; regulation of endothelial cell proliferation; in utero embryonic development; positive regulation of blood vessel endothelial cell migration; positive regulation of peptidyl-serine phosphorylation; cell-substrate adhesion; negative regulation of cytokine secretion during immune response; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; heparin biosynthetic process; positive regulation of protein ubiquitination; regulation of protein binding; negative regulation of vascular permeability; regulation of tumor necrosis factor production; regulation of satellite cell proliferation; sprouting angiogenesis; blood coagulation; leukocyte migration; negative regulation of apoptosis

0.1 mg

245 USD