catalog number :
MBS142293
products type :
Recombinant Protein
products full name :
Recombinant Human Interleukin-3, Sf9
products short name :
Interleukin-3
products name syn :
IL 3 Human, Sf9; Interleukin-3 Human Recombinant, Sf9; MCGF (Mast cell growth factor); Multi-CSF; HCGF; P-cell stimulation factor; IL-3; MGC79398; MGC79399; IL 3 Sf9
other names :
interleukin-3; Interleukin-3; interleukin-3; P-cell stimulating factor; P-cell-stimulating factor; colony-stimulating factor, multiple; hematopoietic growth factor; mast cell growth factor; mast-cell growth factor; multilineage-colony-stimulating factor; multipotential colony-stimulating factor; interleukin 3; Hematopoietic growth factor; Mast cell growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor
products gene name :
IL 3
other gene names :
IL3; IL3; IL-3; MCGF; MULTI-CSF; IL-3; MCGF
uniprot entry name :
IL3_HUMAN
sequence :
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Pro-Thr.
purity :
Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
form :
Lyophilized from a concentrated (1mg/ml) solution in water containing 0.5X PBS pH-7.4. Sterile Filtered White lyophilized (freeze-dried) powder.
storage stability :
Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution IL3 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
other info2 :
Solubility: It is recommended to reconstitute the lyophilized Interleukin-3 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: The ED50 as determined by the dose-dependant stimulation of TF-1 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 10,000,000IU/mg.
products categories :
CYTOKINES AND GROWTH FACTORS; Cytokines; Interleukin
products description :
Description: Interleukin-3 Human Recombinant produced in insect cells is a single, glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15000 Dalton. The IL-3 CSF is fused to a C-terminal His-tag (6x His) and purified by proprietary chromatographic techniques. Introduction: IL3 is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
ncbi acc num :
NP_000579.2
ncbi gb acc num :
NM_000588.3
ncbi mol weight :
17,233 Da
ncbi pathways :
Apoptosis Pathway 83060!!Apoptosis Pathway 470!!Asthma Pathway 83120!!Asthma Pathway 532!!Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway 137993!!Calcium Signaling In The CD4+ TCR Pathway 137941!!Cytokine Signaling In Immune System Pathway 366171!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Fc Epsilon RI Signaling Pathway 83082
ncbi summary :
The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq, Jul 2008]