Recombinant Human Tumor Necrosis Factor-alpha, Mutant | MyBioSource MBS142261 product information

product summary

request information :

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Tumor Necrosis Factor-alpha, Mutant

catalog :

MBS142261

quantity :

0.01 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142261

products type :

Recombinant Protein

products full name :

Recombinant Human Tumor Necrosis Factor-alpha, Mutant

products short name :

Tumor Necrosis Factor-alpha

products name syn :

TNF a Mutant Human; Tumor Necrosis Factor-Alpha Mutant Human Recombinant; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Cachectin; DIF; TNFA; TNFSF2; TNF a Mutant

other names :

tumor necrosis factor; Tumor necrosis factor; tumor necrosis factor; APC1 protein; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; cachectin; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha; tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a

products gene name :

TNF a

other gene names :

TNF; TNF; DIF; TNFA; TNFSF2; TNF-alpha; TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2

uniprot entry name :

TNFA_HUMAN

host :

E Coli

sequence length :

233

sequence :

MRKRKPVAHV VANPQAEGQL QWLNRRANAL LANGVELRDNQLVVPSEGLY LIYSQVLFKG QGCPSTHVLL THTISRIAVS YQTKVNLLSA IKSPCQRETP EGAEAKPWYE PIYLGGVFQL EKGDRLSAEI NRPDYLDFAE SGQVYFGIIAF.

purity :

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

form :

The protein was lyophilized after extensive dialysis against 0.5x PBS pH -7. Sterile Filtered White lyophilized (freeze-dried) powder.

storage stability :

Lyophilized Tumor Necrosis Factor-a Variant although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution TNF-a Variant should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

other info2 :

Solubility: It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha Variant in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is < 0.05ng/ml, corresponding to a Specific Activity of 20,000,000 units/mg.

products categories :

CYTOKINES AND GROWTH FACTORS; Cytokines; Tumor Necrosis Factor

products description :

Description: Tumor Necrosis Factor-a Variant Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 151 amino acids and having a molecular mass of 16598 Dalton. The TNF-alpha Variant is purified by standard chromatographic techniques. Introduction: The clinical use of the potent anti-tumor activity of TNF-a has been limited by the proinflammatory side effects including fever, dose-limiting hypotension, hepatotoxicity, intravascular thrombosis, and hemorrhage. Designing clinically applicable TNF-a mutants with low systemic toxicity has been an intense pharmacological interest. Human TNF-a, which binds to the murine TNF-R55 but not to the mouse TNF-R75, exhibits retained anti-tumor activity and reduced systemic toxicity in mice compared with murine TNF-a, which binds to both murine TNF receptors. Based on these results, many TNF-? mutants that selectively bind to TNF-R55 have been designed. These mutants displayed cytotoxic activities on tumor cell lines in vitro, and exhibited lower systemic toxicity in vivo. Recombinant Human TNF-a Variant/Mutant compared with the wild-type, has an amino acid sequence deletion from a.a. 1-7, and the following a.a. substitutes Arg8, Lys9, Arg10 and Phe157 which is proven tohave more activity and with less inflammatory side effect in vivo.

ncbi gi num :

25952111

ncbi acc num :

NP_000585.2

ncbi gb acc num :

NM_000594.3

uniprot acc num :

P01375

ncbi mol weight :

25,644 Da

ncbi pathways :

Adipocytokine Signaling Pathway 83093!!Adipocytokine Signaling Pathway 505!!Adipogenesis Pathway 198832!!African Trypanosomiasis Pathway 194384!!African Trypanosomiasis Pathway 194323!!AhR Pathway 755436!!Allograft Rejection Pathway 920963!!Allograft Rejection Pathway 83123!!Allograft Rejection Pathway 535!!Alzheimer's Disease Pathway 83097

ncbi summary :

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]

size :

0.01 mg

price :

140 USD

more info or order :

MyBioSource product webpage