Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor, Pichia | MyBioSource MBS142201 product information

product summary

request information :

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor, Pichia

catalog :

MBS142201

quantity :

0.01 mg

price :

205 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142201

products type :

Recombinant Protein

products full name :

Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor, Pichia

products short name :

Granulocyte Macrophage-Colony Stimulating Factor

products name syn :

GM CSF Human, Pichia; Granulocyte Macrophage-Colony Stimulating Factor Human Recombinant, Pichia; CSF-2; MGI-1GM; GM-CSF; Pluripoietin-alpha; Molgramostin; Sargramostim; MGC131935; MGC138897; GM CSF Pichia

other names :

granulocyte-macrophage colony-stimulating factor; Granulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony-stimulating factor; CSF; granulocyte macrophage-colony stimulating factor; molgramostin; sargramostim; colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; Molgramostin; Sargramostim

products gene name :

other gene names :

CSF2; CSF2; GMCSF; GMCSF; GM-CSF; CSF

uniprot entry name :

CSF2_HUMAN

host :

Pichia Pastoris

sequence length :

144

sequence :

The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser.

purity :

Greater than 97.0% as determined by 1. Analysis by RP-HPLC. 2. Analysis by SDS-PAGE.

form :

The protein was lyophilized from a concentrated (1mg/ml) solution containing 10mM phosphate buffer pH 7.0, 40 mg mannitol and 10 mg sucrose. Sterile Filtered White lyophilized (freeze-dried) powder.

storage stability :

Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution GMCSF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

other info2 :

Solubility: It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is < 0.183 ng/ml, corresponding to a Specific Activity of 5,500,000IU/mg.

products categories :

CYTOKINES AND GROWTH FACTORS; Growth Factors; Colony Stimulating Factor

products description :

Description: Granulocyte Macrophage Colony Stimulating Factor Human Recombinant produced in Yeast is a single, glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 26-32 kDa. rhGMCSF differs from the natural human GM-CSF by a substitution of leucine at position 23 (R to L), and the carbohydrate moiety may be different from the native protein. GM-CSF is purified by proprietary chromatographic techniques. Introduction: GMCSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

ncbi gi num :

27437030

ncbi acc num :

NP_000749.2

ncbi gb acc num :

NM_000758.3

uniprot acc num :

P04141

ncbi mol weight :

16,295 Da

ncbi pathways :

Amoebiasis Pathway 167324!!Amoebiasis Pathway 167191!!Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway 137993!!Calcium Signaling In The CD4+ TCR Pathway 137941!!Cytokine Signaling In Immune System Pathway 366171!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Cytokines And Inflammatory Response Pathway 198794!!Fc Epsilon RI Signaling Pathway 83082!!Fc Epsilon RI Signaling Pathway 493

ncbi summary :

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]

size :

0.01 mg

price :

205 USD

more info or order :

MyBioSource product webpage