This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Anti-CCNB1 / Cyclin B1 Antibody (C-Terminus) LS-C95967
catalog :
LS-C95967
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
immunohistochemistry - paraffin section
citations: 1
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; mouse
Nascimento E, Cox C, MacArthur S, Hussain S, Trotter M, Blanco S, et al. The opposing transcriptional functions of Sin3a and c-Myc are required to maintain tissue homeostasis. Nat Cell Biol. 2011;13:1395-405 pubmed publisher
product information
AntibodyID :
97344
AntibodyName :
LS-C95967
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
Anti-CCNB1 / Cyclin B1 Antibody (C-Terminus) LS-C95967
Specificity :
In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is Ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition. cross reacts with mouse and rat.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
C-Terminus
PresentationDesc :
Antiserum, 0.05% sodium azide.
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
A synthetic peptide derived from C-terminus of human cyclin B1 protein.
PurificationDesc :
Ig fraction
RecommendedStorageDesc :
Store at 2-8°C. Do not freeze.
Gene :
CCNB1 / Cyclin B1
StandardGeneSymbol :
CCNB1
Reactivity :
Mouse, Rat, Human
Usage :
IHC-P (1:50 - 1:100)
UsageText :
Formalin-fixed paraffin-embedded tissues require high temperature antigen retrieval with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Synonyms :
CCNB1, CCNB, Cyclin B1, G2/mitotic-specific cyclin-B1, G2/mitotic-specific cyclin B1
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.