This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
P2RX1 / P2X1 Antibody (aa364-372) LS-C38011
catalog :
LS-C38011
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, rat
application :
immunohistochemistry, immunocytochemistry
product information
AntibodyID :
37519
AntibodyName :
LS-C38011
TargetSpecies :
Rat
Host Species :
Rabbit
Product Name :
P2RX1 / P2X1 Antibody (aa364-372) LS-C38011
Specificity :
Species Reactivity: Reacts with Rat.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa364-372
PresentationDesc :
0.05% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide corresponding to residues 364-372 of the rat P2X1.
PurificationDesc :
Antiserum
IsotypeName :
IgG
Gene :
P2RX1 / P2X1
StandardGeneSymbol :
P2RX1
gene family :
Ion channel
Subfamily :
P2X receptor
Reactivity :
Rat
Usage :
ICC (1:1000), IF, IHC (1:1000)
ShortWebDescription :
P2X1 antibody LS-C38011 is an unconjugated rabbit polyclonal antibody to rat P2X1 (P2RX1) (aa364-372). Validated for ICC, IF and IHC.
UsageText :
Suitable for use in Immunohistochemistry, Immunocytochemistry. Immunohistochemistry: 1:1000. Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode's solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4?C with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy. Immunocytochemistry: 1:1000. P2X1 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as described above. Note: Sodium azide interferes with peroxidase reactions and should not be used with peroxidase methodologies. If sodium azide is present in any steps of the staining procedure, the tissue should thoroughly be rinsed with sodium azide-free buffer before performing the peroxidase reaction.
Synonyms :
P2RX1, ATP receptor, p2X receptor 1, p2X1, p2X1 purinoceptor, p2X1 receptor, Purinergic receptor, p2RX1, p2X purinoceptor 1, p2X receptor, subunit 1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.