This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
ECT2 Antibody (phospho-Thr790) LS-C19111
catalog :
LS-C19111
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
human
application :
western blot, ELISA, immunoprecipitation
product information
AntibodyID :
19859
AntibodyName :
LS-C19111
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
ECT2 Antibody (phospho-Thr790) LS-C19111
Specificity :
Reactivity occurs against human ECT2 protein at the pT790 residue. By ELISA the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human ECT2 is minimal by ELISA, but western blot shows specific staining of interphase cell lysates, which are predicted to contain non-phosphorylated ECT2. Additional experiments are underway to clarify antibody specificity. A BLAST analysis was used to suggest cross reactivity with ECT2 protein from human, mouse, rat, zebrafish, chimpanzee, chicken, and dog based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
pThr790
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ECT2 protein.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
ECT2
StandardGeneSymbol :
ECT2
Reactivity :
Chicken, Zebrafish, Rat, Mouse, Dog, Human, Chimpanzee
Usage :
ELISA (1:10000 - 1:40000), IP, WB (1:1000 - 1:4000)
ShortWebDescription :
ECT2 antibody LS-C19111 is an unconjugated rabbit polyclonal antibody to ECT2 (pThr790) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IP and WB.
UsageText :
This affinity-purified antibody has been tested for use in ELISA, western blot and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 101 kD in size by western blotting in the appropriate cell lysate or extract. Less than 2% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is believed to be phospho specific for ECT2 phosphorylated at the pT790 residue. Experiments to generate more conclusive evidence are currently under preparation.
Synonyms :
ECT2, ARHGEF31, Protein ECT2
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.