product summary
request information :
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Anti-Calbindin
catalog :
LS-C125228
quantity :
price :
clonality :
polyclonal
host :
rabbit
reactivity :
human, mouse
application :
western blot, ELISA
product information
?AntibodyID :
128848
AntibodyName :
LS-C125228
TargetSpecies :
mouse
Host Species :
rabbit
Product Name :
Anti-Calbindin
Specificity :
The peptide is 95% conserved in rat, human, rabbit, chicken, bovine D28K. No significant sequence homology is seen with D9K, calretinin or other CABPs.
ClonalityDesc :
polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
C-Terminus
PresentationDesc :
PBS, pH 7.4, 0.1% sodium azide, 40% glycerol
ImmunogenDesc :
A 20-aa peptide sequence, within the C-terminal, non-calcium binding domain of mouse D28K (1) was synthesized, coupled to KLH.
PurificationDesc :
Immunoaffinity Chromatography
RecommendedStorageDesc :
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C.
IsotypeName :
IgG
gene :
Calbindin
StandardGeneSymbol :
CALB1
reactivity :
mouse
Usage :
ELISA (1:10000 - 1:100000), WB (1:1000 - 1:5000)
UsageText :
Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:5000 for neat serum and 1-10 ug/ml for affinity pure using ECL. D28K is ~28kD. ELISA: 1:10000-1:100000 using 50-100 ng of control peptide/well. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of affinity purified IgG or 1 ul antiserum) to confirm antibody specificity.
Synonyms :
CALB1, Calbindin D28, Calbindin, Calbindin 1, 28kDa, CAB27, CALB, RTVL-H protein, Calbindin 1, (28kD), D-28K
SwissProtProtoID :
P05937
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
www.lsbio.com
1-206-464-1554
Headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.