This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Anti-Apolipoprotein B / APOB Antibody IHC-plus™ LS-B2616
catalog :
LS-B2616
clonality :
polyclonal
host :
goat
reactivity :
human
application :
western blot, ELISA, immunoprecipitation, immunohistochemistry - paraffin section
product information
?AntibodyID :
86122
AntibodyName :
LS-B2616
TargetSpecies :
Human
Host Species :
Goat
Product Name :
Anti-Apolipoprotein B / APOB Antibody IHC-plus™ LS-B2616
Specificity :
Recognizes human apolipoprotein B. Assay by IEP resulted in a single precipitin arc against anti goat serum. Has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apolipoproteins. Typically less than 1% cross-reactivity against other types of apolipoprotein was detected by ELISA against purified standards. Non-specific cross-reaction of anti-apolipoprotein antibodies with other human serum proteins is negligible.
ClonalityDesc :
Polyclonal
PresentationDesc :
0.125M sodium borate, 0.075M sodium chloride, 0.005M EDTA, pH 8.0, 0.01% sodium azide, before the addition of glycerol to 40%. No stabilizing proteins added
ImmunogenDesc :
Human Apolipoprotein Type B (isolated from human plasma by density gradient centrifugation followed by HPLC purification).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
IsotypeName :
IgG
Gene :
Apolipoprotein B / APOB
StandardGeneSymbol :
APOB
Reactivity :
Human
Usage :
ELISA (1:1000 - 1:5000), GS, IHC-P (5 µg/ml), IP, WB (1:1000 - 1:5000)
UsageText :
Immunohistochemistry: LS-B2616 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2616 was determined to be 5 ug/ml.
Synonyms :
APOB, ApoB-100, Apolipoprotein B-48, ApoB-48, Apolipoprotein B, FLDB, LDLCQ4, Mutant Apo B 100, Apo B-100, Apolipoprotein B-100, Apolipoprotein B48
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.