product summary
request information :
company name :
LifeSpan Biosciences
product type :
product name :
Anti-ER Alpha / Estrogen Receptor Antibody (aa410-592) IHC-plus™ LS-B1578
catalog :
clonality :
host :
reactivity :
application :
western blot, immunohistochemistry - paraffin section
image 1 :
LifeSpan Biosciences LS-B1578 image 1
Human Breast (formalin-fixed, paraffin-embedded) stained with ESR1 antibody LS-B1578 at 1:800 followed by biotinylated anti-mouse IgG secondary antibody LS-D2, alkaline phosphatase-streptavidin and chromogen.
product information
?AntibodyID :
AntibodyName :
TargetSpecies :
Host Species :
Product Name :
Anti-ER Alpha / Estrogen Receptor Antibody (aa410-592) IHC-plus™ LS-B1578
Specificity :
Ni-NTA purified recombinant human ER-alpha (410-592aa) expressed in E. Coli strain BL21 (DE3)
ClonalityDesc :
AntigenModification :
PresentationDesc :
PBS, pH 7.4, 0.03% sodium azide.
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Recombinant human ESR1 / Estrogen receptor.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
+4°C or -20°C, Avoid repeated freezing and thawing.
IsotypeName :
Gene :
ER Alpha / Estrogen Receptor
StandardGeneSymbol :
gene family :
Subfamily :
NR3 Steroid receptor
Reactivity :
Usage :
IHC-P (1:800), WB (1:500 - 1:2000)
UsageText :
Immunohistochemistry: LS-B1578 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1578 was determined to be 1:800.
Synonyms :
ESR1, Era, ERalpha, Estrogen receptor, Estradiol receptor, ER-alpha, Estrogen receptor 1, NR3A1, ER, ESR, ESRA, Estrogen receptor alpha
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.