product summary
request information :
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Anti-ER Alpha / Estrogen Receptor
catalog :
LS-B1578
quantity :
price :
clonality :
monoclonal
host :
mouse
reactivity :
human
application :
immunohistochemistry - paraffin section, western blot, immunohistochemistry
image
image 1 :
LifeSpan Biosciences LS-B1578 image 1
Human Breast (formalin-fixed, paraffin-embedded) stained with ESR1 antibody LS-B1578 at 1:800 followed by biotinylated anti-mouse IgG secondary antibody LS-D2, alkaline phosphatase-streptavidin and chromogen.
product information
?AntibodyID :
47084
AntibodyName :
LS-B1578
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
Anti-ER Alpha / Estrogen Receptor
Specificity :
Ni-NTA purified recombinant human ER-alpha (410-592aa) expressed in E. Coli strain BL21 (DE3)
ClonalityDesc :
Monoclonal
AntigenModification :
aa410-592
PresentationDesc :
PBS, pH 7.4, 0.03% sodium azide.
ImmunogenDesc :
Recombinant human ESR1 / Estrogen receptor.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
+4°C or -20°C, Avoid repeated freezing and thawing.
IsotypeName :
IgG1
Gene :
ER Alpha / Estrogen Receptor
StandardGeneSymbol :
ESR1
gene family :
NHR
Subfamily :
NR3 Steroid receptor
Reactivity :
Human
Usage :
IHC-P (1:800), WB (1:500 - 1:2000)
UsageText :
Immunohistochemistry: LS-B1578 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1578 was determined to be 1:800.
Synonyms :
ESR1, Era, ERalpha, Estrogen receptor, Estradiol receptor, ER-alpha, Estrogen receptor 1, NR3A1, ER, ESR, ESRA, Estrogen receptor alpha
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
www.lsbio.com
1-206-464-1554
headquarters: usa
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.