MTCO2 Monoclonal Antibody (12C4F12) | Invitrogen A-6404 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

MTCO2 Monoclonal Antibody (12C4F12)

catalog :

A-6404

quantity :

100µg

price :

US 349.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

12C4F12

reactivity :

African green monkey, human, mouse, rat, chicken, cow

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, other

more info or order :

Invitrogen product webpage

citations: 58

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 5a	Lu Y, Acoba M, Selvaraju K, Huang T, Nirujogi R, Sathe G, et al. Human adenine nucleotide translocases physically and functionally interact with respirasomes.. Mol Biol Cell. 2017;28:1489-1506 pubmed publisher
western blot; human; loading ...; fig 2c	Rozanska A, Richter-Dennerlein R, Rorbach J, Gao F, Lewis R, Chrzanowska-Lightowlers Z, et al. The human RNA-binding protein RBFA promotes the maturation of the mitochondrial ribosome.. Biochem J. 2017;474:2145-2158 pubmed publisher
western blot; human; loading ...; fig 3a	Moren C, González-Casacuberta I, Alvarez-Fernández C, Bano M, Catalán-Garcia M, Guitart-Mampel M, et al. HIV-1 promonocytic and lymphoid cell lines: an in vitro model of in vivo mitochondrial and apoptotic lesion. J Cell Mol Med. 2017;21:402-409 pubmed publisher
immunocytochemistry; human; 1:500; fig 4d	Dai D, Danoviz M, Wiczer B, Laflamme M, Tian R. Mitochondrial Maturation in Human Pluripotent Stem Cell Derived Cardiomyocytes.. Stem Cells Int. 2017;2017:5153625 pubmed publisher
western blot; human; loading ...; fig 3d	Maughan R, Feeney E, Capel E, Capeau J, Domingo P, Giralt M, et al. Improved adipose tissue function with initiation of protease inhibitor-only ART. J Antimicrob Chemother. 2016;71:3212-3221 pubmed
western blot; mouse; fig 6	Suliman H, Zobi F, Piantadosi C. Heme Oxygenase-1/Carbon Monoxide System and Embryonic Stem Cell Differentiation and Maturation into Cardiomyocytes. Antioxid Redox Signal. 2016;24:345-60 pubmed publisher
western blot; human; loading ...; fig 4f	Stroud D, Surgenor E, Formosa L, Reljic B, Frazier A, Dibley M, et al. Accessory subunits are integral for assembly and function of human mitochondrial complex I. Nature. 2016;538:123-126 pubmed publisher
western blot; human; loading ...	Yen H, Liu Y, Kan C, Wei H, Lee S, Wei Y, et al. Disruption of the human COQ5-containing protein complex is associated with diminished coenzyme Q10 levels under two different conditions of mitochondrial energy deficiency. Biochim Biophys Acta. 2016;1860:1864-76 pubmed publisher
western blot; mouse; fig 2	Molla B, Riveiro F, Bolinches-Amorós A, Muñoz-Lasso D, Palau F, Gonzalez-Cabo P. Two different pathogenic mechanisms, dying-back axonal neuropathy and pancreatic senescence, are present in the YG8R mouse model of Friedreich's ataxia. Dis Model Mech. 2016;9:647-57 pubmed publisher
western blot; human; 1:10,000; fig 3	Leucci E, Vendramin R, Spinazzi M, Laurette P, Fiers M, Wouters J, et al. Melanoma addiction to the long non-coding RNA SAMMSON. Nature. 2016;531:518-22 pubmed publisher
western blot; human; fig 3	Sakabe I, Hu R, Jin L, Clarke R, Kasid U. TMEM33: a new stress-inducible endoplasmic reticulum transmembrane protein and modulator of the unfolded protein response signaling. Breast Cancer Res Treat. 2015;153:285-97 pubmed publisher
western blot; human	Lee Y, Jee B, Kwon S, Yoon Y, Xu W, Wang H, et al. Identification of a mitochondrial defect gene signature reveals NUPR1 as a key regulator of liver cancer progression. Hepatology. 2015;62:1174-89 pubmed publisher
western blot; human; fig 7	Martínez-Zamora A, Meseguer S, Esteve J, Villarroya M, Aguado C, Enríquez J, et al. Defective Expression of the Mitochondrial-tRNA Modifying Enzyme GTPBP3 Triggers AMPK-Mediated Adaptive Responses Involving Complex I Assembly Factors, Uncoupling Protein 2, and the Mitochondrial Pyruvate Carrier. PLoS ONE. 2015;10:e0144273 pubmed publisher
western blot; human	Lee J, Lee Y, Lim J, Byun H, Park I, Kim G, et al. Mitochondrial Respiratory Dysfunction Induces Claudin-1 Expression via Reactive Oxygen Species-mediated Heat Shock Factor 1 Activation, Leading to Hepatoma Cell Invasiveness. J Biol Chem. 2015;290:21421-31 pubmed publisher
western blot; human	Oláhová M, Haack T, Alston C, Houghton J, He L, Morris A, et al. A truncating PET100 variant causing fatal infantile lactic acidosis and isolated cytochrome c oxidase deficiency. Eur J Hum Genet. 2015;23:935-9 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1000	Selfridge J, Wilkins H, E L, Carl S, Koppel S, Funk E, et al. Effect of one month duration ketogenic and non-ketogenic high fat diets on mouse brain bioenergetic infrastructure. J Bioenerg Biomembr. 2015;47:1-11 pubmed publisher
western blot; human; fig 2	Lauková J, Kozubik A, Hofmanova J, Nekvindova J, Sova P, Moyer M, et al. Loss of PTEN Facilitates Rosiglitazone-Mediated Enhancement of Platinum(IV) Complex LA-12-Induced Apoptosis in Colon Cancer Cells. PLoS ONE. 2015;10:e0141020 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:100	Garone C, García-Diaz B, Emmanuele V, Lopez L, Tadesse S, Akman H, et al. Deoxypyrimidine monophosphate bypass therapy for thymidine kinase 2 deficiency. EMBO Mol Med. 2014;6:1016-27 pubmed publisher
immunohistochemistry; rat; fig 2	Sadacharan S, Singh B, Bowes T, Gupta R. Localization of mitochondrial DNA encoded cytochrome c oxidase subunits I and II in rat pancreatic zymogen granules and pituitary growth hormone granules. Histochem Cell Biol. 2005;124:409-21 pubmed
western blot; human	Guo F, Sigua C, Tao J, Bali P, George P, Li Y, et al. Cotreatment with histone deacetylase inhibitor LAQ824 enhances Apo-2L/tumor necrosis factor-related apoptosis inducing ligand-induced death inducing signaling complex activity and apoptosis of human acute leukemia cells. Cancer Res. 2004;64:2580-9 pubmed
western blot; human	Mazzanti R, Giulivi C. Coordination of nuclear- and mitochondrial-DNA encoded proteins in cancer and normal colon tissues. Biochim Biophys Acta. 2006;1757:618-23 pubmed
western blot; human; fig 4	Gianni P, Jan K, Douglas M, Stuart P, Tarnopolsky M. Oxidative stress and the mitochondrial theory of aging in human skeletal muscle. Exp Gerontol. 2004;39:1391-400 pubmed
western blot; human	Ngamsiri P, Watcharasit P, Satayavivad J. Glycogen synthase kinase-3 (GSK3) controls deoxyglucose-induced mitochondrial biogenesis in human neuroblastoma SH-SY5Y cells. Mitochondrion. 2014;14:54-63 pubmed publisher
western blot; mouse; fig 1b	Cho S, Shin E, Park P, Shin D, Chang H, Kim D, et al. Identification of mouse Prp19p as a lipid droplet-associated protein and its possible involvement in the biogenesis of lipid droplets. J Biol Chem. 2007;282:2456-65 pubmed
other; human; fig 3	Esposti M, Erler J, Hickman J, Dive C. Bid, a widely expressed proapoptotic protein of the Bcl-2 family, displays lipid transfer activity. Mol Cell Biol. 2001;21:7268-76 pubmed
western blot; human	Almalki A, Alston C, Parker A, Simonic I, Mehta S, He L, et al. Mutation of the human mitochondrial phenylalanine-tRNA synthetase causes infantile-onset epilepsy and cytochrome c oxidase deficiency. Biochim Biophys Acta. 2014;1842:56-64 pubmed publisher
immunohistochemistry - paraffin section; human; 1:200 immunohistochemistry - frozen section; African green monkey; 1:100	Horn A, Eberhorn A, Hartig W, Ardeleanu P, Messoudi A, Büttner-Ennever J. Perioculomotor cell groups in monkey and man defined by their histochemical and functional properties: reappraisal of the Edinger-Westphal nucleus. J Comp Neurol. 2008;507:1317-35 pubmed publisher
immunocytochemistry; African green monkey; fig 5	Bhagwat S, Biswas G, Anandatheerthavarada H, Addya S, Pandak W, Avadhani N. Dual targeting property of the N-terminal signal sequence of P4501A1. Targeting of heterologous proteins to endoplasmic reticulum and mitochondria. J Biol Chem. 1999;274:24014-22 pubmed
western blot; human; fig 6	Guo W, Shiina I, Wang Y, Umeda E, Watanabe C, Uetake S, et al. Ridaifen-SB8, a novel tamoxifen derivative, induces apoptosis via reactive oxygen species-dependent signaling pathway. Biochem Pharmacol. 2013;86:1272-84 pubmed publisher
western blot; human; fig 2	Mazzanti R, Solazzo M, Fantappiè O, Elfering S, Pantaleo P, Bechi P, et al. Differential expression proteomics of human colon cancer. Am J Physiol Gastrointest Liver Physiol. 2006;290:G1329-38 pubmed
western blot; human; 1:2000	Balasubramanian S, Ramos J, Luo W, Sirisawad M, Verner E, Buggy J. A novel histone deacetylase 8 (HDAC8)-specific inhibitor PCI-34051 induces apoptosis in T-cell lymphomas. Leukemia. 2008;22:1026-34 pubmed publisher
western blot; human; fig 4	Dennerlein S, Rozanska A, Wydro M, Chrzanowska-Lightowlers Z, Lightowlers R. Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S small mitochondrial ribosomal subunit. Biochem J. 2010;430:551-8 pubmed publisher
western blot; mouse	Cheung N, Choy M, Halliwell B, Teo T, Bay B, Lee A, et al. Lactacystin-induced apoptosis of cultured mouse cortical neurons is associated with accumulation of PTEN in the detergent-resistant membrane fraction. Cell Mol Life Sci. 2004;61:1926-34 pubmed
western blot; human; fig 10	Gajate C, An F, Mollinedo F. Differential cytostatic and apoptotic effects of ecteinascidin-743 in cancer cells. Transcription-dependent cell cycle arrest and transcription-independent JNK and mitochondrial mediated apoptosis. J Biol Chem. 2002;277:41580-9 pubmed
immunocytochemistry; human; fig 3	Tchakarska G, Roussel M, Troussard X, Sola B. Cyclin D1 inhibits mitochondrial activity in B cells. Cancer Res. 2011;71:1690-9 pubmed publisher
western blot; rat	Das S, Bedja D, Campbell N, Dunkerly B, Chenna V, Maitra A, et al. miR-181c regulates the mitochondrial genome, bioenergetics, and propensity for heart failure in vivo. PLoS ONE. 2014;9:e96820 pubmed publisher
western blot; human; fig 3	Geserick P, Wang J, Feoktistova M, Leverkus M. The ratio of Mcl-1 and Noxa determines ABT737 resistance in squamous cell carcinoma of the skin. Cell Death Dis. 2014;5:e1412 pubmed publisher
western blot; human; fig 2	Len A, Powner M, Zhu L, Hageman G, Song X, Fruttiger M, et al. Pilot application of iTRAQ to the retinal disease Macular Telangiectasia. J Proteome Res. 2012;11:537-53 pubmed publisher
western blot; human; fig 3	Nyfeler B, Hoepfner D, Palestrant D, Kirby C, Whitehead L, Yu R, et al. Identification of elongation factor G as the conserved cellular target of argyrin B. PLoS ONE. 2012;7:e42657 pubmed publisher
western blot; human	Villarroya J, de Bolos C, Meseguer A, Hirano M, Vila M. Altered gene transcription profiles in fibroblasts harboring either TK2 or DGUOK mutations indicate compensatory mechanisms. Exp Cell Res. 2009;315:1429-38 pubmed publisher
western blot; human; fig 2	Tucker E, Hershman S, Köhrer C, Belcher-Timme C, Patel J, Goldberger O, et al. Mutations in MTFMT underlie a human disorder of formylation causing impaired mitochondrial translation. Cell Metab. 2011;14:428-34 pubmed publisher
western blot; human	Rokhlin O, Glover R, Taghiyev A, Guseva N, Seftor R, Shyshynova I, et al. Bisindolylmaleimide IX facilitates tumor necrosis factor receptor family-mediated cell death and acts as an inhibitor of transcription. J Biol Chem. 2002;277:33213-9 pubmed
western blot; human	Clohessy J, Zhuang J, de Boer J, Gil-Gomez G, Brady H. Mcl-1 interacts with truncated Bid and inhibits its induction of cytochrome c release and its role in receptor-mediated apoptosis. J Biol Chem. 2006;281:5750-9 pubmed
western blot; cow western blot; human	Capaldi R, Marusich M, Taanman J. Mammalian cytochrome-c oxidase: characterization of enzyme and immunological detection of subunits in tissue extracts and whole cells. Methods Enzymol. 1995;260:117-32 pubmed
western blot; mouse	Wolff N, Ghio A, Garrick L, Garrick M, Zhao L, Fenton R, et al. Evidence for mitochondrial localization of divalent metal transporter 1 (DMT1). FASEB J. 2014;28:2134-45 pubmed publisher
western blot; human	Narvaez C, Welsh J. Role of mitochondria and caspases in vitamin D-mediated apoptosis of MCF-7 breast cancer cells. J Biol Chem. 2001;276:9101-7 pubmed
western blot; chicken; fig 1	Solomonia R, Kunelauri N, Mikautadze E, Apkhazava D, McCabe B, Horn G. Mitochondrial proteins, learning and memory: biochemical specialization of a memory system. Neuroscience. 2011;194:112-23 pubmed publisher
western blot; mouse; 1:1000	Ribeiro M, Rosenstock T, Oliveira A, Oliveira C, Rego A. Insulin and IGF-1 improve mitochondrial function in a PI-3K/Akt-dependent manner and reduce mitochondrial generation of reactive oxygen species in Huntington's disease knock-in striatal cells. Free Radic Biol Med. 2014;74:129-44 pubmed publisher
western blot; rat	Ungvari Z, Labinskyy N, Gupte S, Chander P, Edwards J, Csiszar A. Dysregulation of mitochondrial biogenesis in vascular endothelial and smooth muscle cells of aged rats. Am J Physiol Heart Circ Physiol. 2008;294:H2121-8 pubmed publisher
western blot; rat	Rodrigues C, Stieers C, Keene C, Ma X, Kren B, Low W, et al. Tauroursodeoxycholic acid partially prevents apoptosis induced by 3-nitropropionic acid: evidence for a mitochondrial pathway independent of the permeability transition. J Neurochem. 2000;75:2368-79 pubmed
western blot; human	Yee K, Wilkinson S, James J, Ryan K, Vousden K. PUMA- and Bax-induced autophagy contributes to apoptosis. Cell Death Differ. 2009;16:1135-45 pubmed publisher
	Parry L, Young M, el Marjou F, Clarke A. Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models. J Vis Exp. 2015;: pubmed publisher
	Jiang B, Xiao W, Shi Y, Liu M, Xiao X. Heat shock pretreatment inhibited the release of Smac/DIABLO from mitochondria and apoptosis induced by hydrogen peroxide in cardiomyocytes and C2C12 myogenic cells. Cell Stress Chaperones. 2005;10:252-62 pubmed
	Syken J, De-Medina T, Munger K. TID1, a human homolog of the Drosophila tumor suppressor l(2)tid, encodes two mitochondrial modulators of apoptosis with opposing functions. Proc Natl Acad Sci U S A. 1999;96:8499-504 pubmed
	Bereziat V, Cervera P, Le Dour C, Verpont M, Dumont S, Vantyghem M, et al. LMNA mutations induce a non-inflammatory fibrosis and a brown fat-like dystrophy of enlarged cervical adipose tissue. Am J Pathol. 2011;179:2443-53 pubmed publisher
	Mosser D, Caron A, Bourget L, Meriin A, Sherman M, Morimoto R, et al. The chaperone function of hsp70 is required for protection against stress-induced apoptosis. Mol Cell Biol. 2000;20:7146-59 pubmed
	Ussakli C, Ebaee A, Binkley J, Brentnall T, Emond M, Rabinovitch P, et al. Mitochondria and tumor progression in ulcerative colitis. J Natl Cancer Inst. 2013;105:1239-48 pubmed publisher
	Stiburek L, Vesela K, Hansikova H, Pecina P, Tesarova M, Cerna L, et al. Tissue-specific cytochrome c oxidase assembly defects due to mutations in SCO2 and SURF1. Biochem J. 2005;392:625-32 pubmed

product information

Product Type :

Antibody

Product Name :

MTCO2 Monoclonal Antibody (12C4F12)

Catalog # :

A-6404

Quantity :

100µg

Price :

US 349.00

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Human

Applications :

Immunocytochemistry: 5-10 µg/mL, Immunofluorescence: 5-10 µg/mL, Western Blot: 1 µg/mL

Species :

Human

Clone :

12C4F12

Isotype :

IgG2a, kappa

Storage :

4° C, do not freeze

Description :

Oxidative phosphorylation (OxPhos) occurs in mitochondria and, in mammals, is catalyzed by five membrane-bound protein complexes, namely NADH-ubiquinol oxidoreductase (Complex I), succinate-ubiquinol oxidoreductase (Complex II), ubiquinol-cytochrome c oxidoreductase (Complex III), cytochrome c oxidase (COX) (Complex IV), and ATP synthase (Complex V). The complexes are composed of multiple subunits, some of which are encoded in the mitochondria and others in the nucleus. Assembly of each complex involves a coordinated association of prosthetic groups with the polypeptides. This complicated process requires assembly factors specific for each complex. Defects in assembly of one or more of these complexes contribute to several described mitochondrial diseases and possibly Alzheimer's and Parkinson's diseases. Cytochrome c oxidase (COX), the fourth complex in the respiratory chain, is responsible for catalyzing the conversion of O2 to H2O. Subunit 2 of the cytochrome c oxidase complex combines with two other subunits (1 and 3) to form a core protein structure that performs many functions of the enzyme. The metallic copper center of this subunit transfers electrons to the heme center of subunit 1, which results in the movement of electrons from cytochrome c to the heme A3 and copper B metallic center of complex IV. COX catalyzes the transfer of electrons from reduced cytochrome c to molecular oxygen, with a concomitant translocation of protons across the mitochondrial inner membrane. This inner mitochondrial membrane-bound enzyme is composed of subunits that are encoded in both the mitochondria (subunits I, II, and III) and the nucleus (ten others). Defects in subunit 2 of cytochrome c oxidase can result in COX deficiency, which causes a wide range of symptoms from local myopathy to multiple system pathologies that begin between infancy and adulthood. Abnormalities in this subunit are also associated with tumor development.

Immunogen :

Full-length native Human Cytochrome C oxidase (Complex IV) subunit II, from SDS-PAGE

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 5-10 µg/mL, Immunofluorescence: 5-10 µg/mL, Western Blot: 1 µg/mL

Aliases :

MTCO2, MT-CO2, Cytochrome c oxidase 2, Cytochrome c oxidase II, Cytochrome c oxidase polypeptide II, COII, COXII

PubMed References :

10411904, 10446170, 10982831, 11053435, 11080188, 11585909, 12091392, 12198119, 15059915, 15289934, 15489062, 16083427, 16133117, 16184770, 16380381, 16439467, 16730322, 17118936, 18186030, 18256683, 18326800, 19265691, 19300452, 20604745, 21343394, 21839805, 21907147, 21945321, 22098468, 22970117, 23852949, 23973528, 24161539, 24316184, 24448823, 24810628, 24968719, 24992836, 25104046, 25210795, 25293719, 26157141, 26173068, 26268696, 26492315, 26642043, 26725491, 27008969, 27079523, 27155576, 27516476, 27626371, 27758070, 28421116, 8592440

more info or order :

Invitrogen product webpage