Phospho-SHC pTyr239 / pTyr240 Polyclonal Antibody | Invitrogen A13980 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon

product type :

antibody

product name :

Phospho-SHC pTyr239 / pTyr240 Polyclonal Antibody

catalog :

A13980

quantity :

100 µl

price :

US 325

clonality :

polyclonal

host :

rabbit

antigen modification :

phosphorylated

reactivity :

human, mouse, rat

application :

western blot

product information

Product Type :

A13980

Product Name :

Phospho-SHC pTyr239 / pTyr240 Polyclonal Antibody

Catalog # :

A13980

Quantity :

100 µl

Price :

US 325

Clonality :

Polyclonal

Purity :

purified

Host :

Rabbit

Reactivity :

Rat, Mouse, Human

Applications :

Western Blot

Species :

Rt, Ms, Hu

Gene Identification :

Entrez: 6464, 20416, 85385; Uniprot: P29353, P98083, Q5M824

Isotype :

IgG

Storage :

-20°C

Description :

Shc is a prototype adapter protein that is expressed from the earliest stages of T-cell development. Shc becomes rapidly tyrosine phosphorylated after T-cell receptor (TCR) engagement. Three shc genes have been identified in mammals and their gene products have been referred to as ShcA, ShcB and ShcC. ShcA is ubiquitously expressed, while shcB and ShcC expression appear limited to neuronal cells. ShcA is expressed as three isoforms of about 46, 52 and 66 kDa. Shc is composed of an N-terminal PTB, a central collagen-homology (CH) domain and a C-terminal SH2 domain. The 66 kDa isoform of ShcA is expressed in most cells except in the hematopoietic lineage and contains an additional amino-terminal CH-like region. The tyrosine phosphorylation of Shc has been noticed upon engagement of numerous cell surface receptors such as growth factor receptors, antigen receptors, cytokine receptors, G-protein coupled receptors and hormone receptors. The involvement of ShcA in the Ras signaling pathway is initiated by the tyrosine-phosphorylation of the receptor protein tyrosine kinases (RPTKs) and subsequent interaction with Grb2 and Ras guanine nucleotide exchange factor, Sos. The Shc : Grb2 : Sos complex gets localized to the membrane through the interaction of Shc with the phosphorylated receptor. Membrane-bound Sos then activates Ras by catalysing GDP/GTD exchange. GTP-bound Ras then triggers downstream events, which include Raf, the mitogen-activated protein kinases (MAPKs) and MAPK kinase (MEK).

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding Tyr239/240 of human Shc

Format :

Liquid; 0.01 M HEPES, 100 µg/ml BSA, 50 % glycerol, 0.15 M NaCl, no preservative

Applications w/Dilutions :

WB 1:1,000

Aliases :

SH2 domain protein C1, SHC, SHC-transforming protein 1, SHCA, Src homology 2 domain-containing-transforming protein C1