Phospho-STAT5 alpha (Tyr694) Polyclonal Antibody | Invitrogen 71-6900 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-STAT5 alpha (Tyr694) Polyclonal Antibody

catalog :

71-6900

quantity :

50µg

price :

US 309.00

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse, rat, cow, sheep

application :

western blot, ELISA, immunohistochemistry, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - free floating section

more info or order :

Invitrogen product webpage

citations: 33

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 4g western blot; mouse; 1:1000; loading ...; fig 4a	Barcus C, O'Leary K, Brockman J, Rugowski D, Liu Y, Garcia N, et al. Elevated collagen-I augments tumor progressive signals, intravasation and metastasis of prolactin-induced estrogen receptor alpha positive mammary tumor cells. Breast Cancer Res. 2017;19:9 pubmed publisher
western blot; human; loading ...; fig 2c	Barcus C, Keely P, Eliceiri K, Schuler L. Prolactin signaling through focal adhesion complexes is amplified by stiff extracellular matrices in breast cancer cells. Oncotarget. 2016;7:48093-48106 pubmed publisher
immunohistochemistry - free floating section; rat; 1:100	Moravcová S, Cervena K, Pačesová D, Bendova Z. Identification of STAT3 and STAT5 proteins in the rat suprachiasmatic nucleus and the Day/Night difference in astrocytic STAT3 phosphorylation in response to lipopolysaccharide. J Neurosci Res. 2016;94:99-108 pubmed publisher
western blot; human; loading ...; fig 1a	Schauwecker S, Kim J, Licht J, Clevenger C. Histone H1 and Chromosomal Protein HMGN2 Regulate Prolactin-Induced STAT5 Transcription Factor Recruitment and Function in Breast Cancer Cells. J Biol Chem. 2016;: pubmed publisher
western blot; mouse; 1 ug/ml; fig 5	Schroeder M, Rose-Hellekant T, Sandgren E, Schuler L. Dysregulation of mammary Stats 1,3 and 5 and PRL receptors by overexpression of TGFalpha. Mol Cell Endocrinol. 2001;175:173-83 pubmed
western blot; mouse	Dubois S, Shou W, Haneline L, Fleischer S, Waldmann T, Müller J. Distinct pathways involving the FK506-binding proteins 12 and 12.6 underlie IL-2-versus IL-15-mediated proliferation of T cells. Proc Natl Acad Sci U S A. 2003;100:14169-74 pubmed
immunohistochemistry - paraffin section; human; 1:40	Cotarla I, Ren S, Zhang Y, Gehan E, Singh B, Furth P. Stat5a is tyrosine phosphorylated and nuclear localized in a high proportion of human breast cancers. Int J Cancer. 2004;108:665-71 pubmed
chromatin immunoprecipitation; mouse	Lin G, LaPensee C, Qin Z, Schwartz J. Reciprocal occupancy of BCL6 and STAT5 on Growth Hormone target genes: contrasting transcriptional outcomes and promoter-specific roles of p300 and HDAC3. Mol Cell Endocrinol. 2014;395:19-31 pubmed publisher
western blot; human	Trenerry M, Carey K, Ward A, Cameron-Smith D. STAT3 signaling is activated in human skeletal muscle following acute resistance exercise. J Appl Physiol (1985). 2007;102:1483-9 pubmed
EMSA; human	Moriggl R, Sexl V, Kenner L, Duntsch C, Stangl K, Gingras S, et al. Stat5 tetramer formation is associated with leukemogenesis. Cancer Cell. 2005;7:87-99 pubmed
western blot; human; 1:4000; fig 3	Wu A, Dalvi P, Lu X, Yang M, Riddick D, Matthews J, et al. Induction of multidrug resistance transporter ABCG2 by prolactin in human breast cancer cells. Mol Pharmacol. 2013;83:377-88 pubmed publisher
western blot; mouse; fig 2	Jin H, Lanning N, Carter-Su C. JAK2, but not Src family kinases, is required for STAT, ERK, and Akt signaling in response to growth hormone in preadipocytes and hepatoma cells. Mol Endocrinol. 2008;22:1825-41 pubmed publisher
western blot; mouse	Blaas L, Kornfeld J, Schramek D, Musteanu M, Zollner G, Gumhold J, et al. Disruption of the growth hormone--signal transducer and activator of transcription 5--insulinlike growth factor 1 axis severely aggravates liver fibrosis in a mouse model of cholestasis. Hepatology. 2010;51:1319-26 pubmed publisher
western blot; mouse; fig 7	Kirabo A, Oh S, Kasahara H, Wagner K, Sayeski P. Vascular smooth muscle Jak2 deletion prevents angiotensin II-mediated neointima formation following injury in mice. J Mol Cell Cardiol. 2011;50:1026-34 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:100; fig 5 western blot; mouse; 1:10000; fig 5	Wu A, Yang M, Dalvi P, Turinsky A, Wang W, Butcher D, et al. Role of STAT5 and epigenetics in lactation-associated upregulation of multidrug transporter ABCG2 in the mammary gland. Am J Physiol Endocrinol Metab. 2014;307:E596-610 pubmed publisher
immunohistochemistry - paraffin section; mouse western blot; mouse; 1:1000	Schlederer M, Mueller K, Haybaeck J, Heider S, Huttary N, Rosner M, et al. Reliable quantification of protein expression and cellular localization in histological sections. PLoS ONE. 2014;9:e100822 pubmed publisher
western blot; human	Barcus C, Keely P, Eliceiri K, Schuler L. Stiff collagen matrices increase tumorigenic prolactin signaling in breast cancer cells. J Biol Chem. 2013;288:12722-32 pubmed publisher
western blot; human; 1:1000; fig 4	Schroeder M, Brockman J, Walker A, Schuler L. Inhibition of prolactin (PRL)-induced proliferative signals in breast cancer cells by a molecular mimic of phosphorylated PRL, S179D-PRL. Endocrinology. 2003;144:5300-7 pubmed
immunohistochemistry; mouse; fig 8b western blot; mouse; fig 8a	Nagy T, Wei H, Shen T, Peng X, Liang C, Gan B, et al. Mammary epithelial-specific deletion of the focal adhesion kinase gene leads to severe lobulo-alveolar hypoplasia and secretory immaturity of the murine mammary gland. J Biol Chem. 2007;282:31766-76 pubmed
western blot; mouse; fig 3A	Engblom D, Kornfeld J, Schwake L, Tronche F, Reimann A, Beug H, et al. Direct glucocorticoid receptor-Stat5 interaction in hepatocytes controls body size and maturation-related gene expression. Genes Dev. 2007;21:1157-62 pubmed
western blot; human; fig 1	Friedbichler K, Kerenyi M, Kovacic B, Li G, Hoelbl A, Yahiaoui S, et al. Stat5a serine 725 and 779 phosphorylation is a prerequisite for hematopoietic transformation. Blood. 2010;116:1548-58 pubmed publisher
western blot; human	Clausen L, Kristiansen M, Rasmussen L, Billestrup N, Blaabjerg O, Ledet T, et al. Growth hormone receptor expression and function in pituitary adenomas. Clin Endocrinol (Oxf). 2004;60:576-83 pubmed
western blot; mouse; fig 2	Mueller K, Kornfeld J, Friedbichler K, Blaas L, Egger G, Esterbauer H, et al. Impairment of hepatic growth hormone and glucocorticoid receptor signaling causes steatosis and hepatocellular carcinoma in mice. Hepatology. 2011;54:1398-409 pubmed publisher
western blot; mouse; 1:50; fig 5c	Wang R, Vadlamudi R, Bagheri-Yarmand R, Beuvink I, Hynes N, Kumar R. Essential functions of p21-activated kinase 1 in morphogenesis and differentiation of mammary glands. J Cell Biol. 2003;161:583-92 pubmed
	Moon P, Kim M, Oh H, Nam S, Han N, Jeong H, et al. Cysteine induces longitudinal bone growth in mice by upregulating IGF-I. Int J Mol Med. 2015;36:571-6 pubmed publisher
	Botbol Y, Patel B, Macian F. Common γ-chain cytokine signaling is required for macroautophagy induction during CD4+ T-cell activation. Autophagy. 2015;11:1864-77 pubmed publisher
	Sanz-Moreno A, Fuhrmann D, Wolf E, von Eyss B, Eilers M, Elsässer H. Miz1 deficiency in the mammary gland causes a lactation defect by attenuated Stat5 expression and phosphorylation. PLoS ONE. 2014;9:e89187 pubmed publisher
	Yang N, Huang Y, Jiang J, Frank S. Caveolar and lipid raft localization of the growth hormone receptor and its signaling elements: impact on growth hormone signaling. J Biol Chem. 2004;279:20898-905 pubmed
	Jackson S, Yu C, Mahdi R, Ebong S, Egwuagu C. Dendritic cell maturation requires STAT1 and is under feedback regulation by suppressors of cytokine signaling. J Immunol. 2004;172:2307-15 pubmed
	Tronche F, Opherk C, Moriggl R, Kellendonk C, Reimann A, Schwake L, et al. Glucocorticoid receptor function in hepatocytes is essential to promote postnatal body growth. Genes Dev. 2004;18:492-7 pubmed
	Zhao H, Pearson E, Brooks D, Coon J, Chen D, Demura M, et al. A humanized pattern of aromatase expression is associated with mammary hyperplasia in mice. Endocrinology. 2012;153:2701-13 pubmed publisher
	Zhang Y, Jiang J, Kopchick J, Frank S. Disulfide linkage of growth hormone (GH) receptors (GHR) reflects GH-induced GHR dimerization. Association of JAK2 with the GHR is enhanced by receptor dimerization. J Biol Chem. 1999;274:33072-84 pubmed
	Zhang Y, Turkson J, Carter-Su C, Smithgall T, Levitzki A, Kraker A, et al. Activation of Stat3 in v-Src-transformed fibroblasts requires cooperation of Jak1 kinase activity. J Biol Chem. 2000;275:24935-44 pubmed

product information

Product Type :

Antibody

Product Name :

Phospho-STAT5 alpha (Tyr694) Polyclonal Antibody

Catalog # :

71-6900

Quantity :

50µg

Price :

US 309.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Bovine, Human, Mouse, Ovine, Rat

Applications :

ELISA: Assay Dependent, Immunohistochemistry: Assay Dependent, Western Blot: Assay Dependent

Species :

Bovine, Human, Mouse, Ovine, Rat

Isotype :

IgG

Storage :

-20°C

Description :

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells.

Immunogen :

Synthetic tyrosine-phosphorylated peptide encompassing the conserved C-terminal tyrosine phosphorylation site (Y694) of murine STAT5. This peptide is conserved in human, bovine, sheep, and rat STAT5 proteins

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay Dependent, Immunohistochemistry: Assay Dependent, Western Blot: Assay Dependent

Aliases :

Signal transducer and activator of transcription 5A, STAT5A, STAT5

PubMed References :

10551877, 10823829, 11325527, 12732616, 12970160, 14605212, 14696092, 14764699, 15010456, 15037546, 15104560, 15652752, 17204573, 17504935, 17716968, 18499741, 20162728, 20508164, 21420414, 21725989, 22508516, 23150485, 23530035, 24586582, 25013898, 25088465, 25117410, 26391567, 26420542, 27344177, 28035005, 28103936

more info or order :

Invitrogen product webpage