Annexin A1 Polyclonal Antibody | Invitrogen 71-3400 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Annexin A1 Polyclonal Antibody

catalog :

71-3400

quantity :

100µL

price :

US 309.00

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, western blot knockout validation, immunocytochemistry knockout validation

more info or order :

Invitrogen product webpage

citations: 17

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry knockout validation; human; 1:100; fig 2 western blot knockout validation; human; 1:10,000; fig 1	Belvedere R, Bizzarro V, Forte G, Dal Piaz F, Parente L, Petrella A. Annexin A1 contributes to pancreatic cancer cell phenotype, behaviour and metastatic potential independently of Formyl Peptide Receptor pathway. Sci Rep. 2016;6:29660 pubmed publisher
western blot; human; 1:10,000; loading ...; fig 2	Bizzarro V, Belvedere R, Migliaro V, Romano E, Parente L, Petrella A. Hypoxia regulates ANXA1 expression to support prostate cancer cell invasion and aggressiveness. Cell Adh Migr. 2016;:1-14 pubmed publisher
western blot; human; loading ...	Hill S, Nesser N, Johnson-Camacho K, Jeffress M, Johnson A, Boniface C, et al. Context Specificity in Causal Signaling Networks Revealed by Phosphoprotein Profiling. Cell Syst. 2016;: pubmed publisher
immunohistochemistry - paraffin section; human; 1:100; fig s3a	de Jong R, Paulin N, Lemnitzer P, Viola J, Winter C, Ferraro B, et al. Protective Aptitude of Annexin A1 in Arterial Neointima Formation in Atherosclerosis-Prone Mice. Arterioscler Thromb Vasc Biol. 2016;: pubmed publisher
western blot; human; 1:1000; fig 3 western blot; mouse; 1:1000; fig 3	Fournier N, Tardivel S, Benoist J, Vedie B, Rousseau-Ralliard D, Nowak M, et al. Eicosapentaenoic acid membrane incorporation impairs ABCA1-dependent cholesterol efflux via a protein kinase A signaling pathway in primary human macrophages. Biochim Biophys Acta. 2016;1861:331-41 pubmed publisher
western blot; human; fig 2g	Colangelo T, Polcaro G, Ziccardi P, Pucci B, Muccillo L, Galgani M, et al. Proteomic screening identifies calreticulin as a miR-27a direct target repressing MHC class I cell surface exposure in colorectal cancer. Cell Death Dis. 2016;7:e2120 pubmed publisher
immunocytochemistry; mouse western blot; mouse; loading ...; fig 2a	de Torre-Minguela C, Barberà-Cremades M, Gómez A, Martín-Sánchez F, Pelegrin P. Macrophage activation and polarization modify P2X7 receptor secretome influencing the inflammatory process. Sci Rep. 2016;6:22586 pubmed publisher
flow cytometry; mouse western blot; mouse	Divangahi M, Chen M, Gan H, Desjardins D, Hickman T, Lee D, et al. Mycobacterium tuberculosis evades macrophage defenses by inhibiting plasma membrane repair. Nat Immunol. 2009;10:899-906 pubmed publisher
western blot; human; 1:1000; fig 6	Severino P, Bruggemann H, Andreghetto F, Camps C, Klingbeil M, de Pereira W, et al. MicroRNA expression profile in head and neck cancer: HOX-cluster embedded microRNA-196a and microRNA-10b dysregulation implicated in cell proliferation. BMC Cancer. 2013;13:533 pubmed publisher
immunocytochemistry; mouse; fig 5	Yona S, Heinsbroek S, Peiser L, Gordon S, Perretti M, Flower R. Impaired phagocytic mechanism in annexin 1 null macrophages. Br J Pharmacol. 2006;148:469-77 pubmed
immunocytochemistry; human; 1:150	Coss K, Treacy E, Cotter E, Knerr I, Murray D, Shin Y, et al. Systemic gene dysregulation in classical Galactosaemia: Is there a central mechanism?. Mol Genet Metab. 2014;113:177-87 pubmed publisher
immunohistochemistry; human; fig 3 immunohistochemistry; mouse; fig 3	Gaspar C, Cardoso J, Franken P, Molenaar L, Morreau H, Möslein G, et al. Cross-species comparison of human and mouse intestinal polyps reveals conserved mechanisms in adenomatous polyposis coli (APC)-driven tumorigenesis. Am J Pathol. 2008;172:1363-80 pubmed publisher
western blot; human; 1:1000; fig 4	Song H, Kwan S, Izaguirre D, Zu Z, Tsang Y, Tung C, et al. PAX2 Expression in Ovarian Cancer. Int J Mol Sci. 2013;14:6090-105 pubmed publisher
ELISA; human; 1:1000	Yazid S, Solito E, Christian H, McArthur S, Goulding N, Flower R. Cromoglycate drugs suppress eicosanoid generation in U937 cells by promoting the release of Anx-A1. Biochem Pharmacol. 2009;77:1814-26 pubmed publisher
	Leoni G, Neumann P, Kamaly N, Quirós M, Nishio H, Jones H, et al. Annexin A1-containing extracellular vesicles and polymeric nanoparticles promote epithelial wound repair.. J Clin Invest. 2015;125:1215-27 pubmed publisher
	Kwon J, Lee J, Kim K, Chung Y, Kim I. Regulation of cytosolic phospholipase A2 phosphorylation by proteolytic cleavage of annexin A1 in activated mast cells. J Immunol. 2012;188:5665-73 pubmed publisher
	Girol A, Mimura K, Drewes C, Bolonheis S, Solito E, Farsky S, et al. Anti-inflammatory mechanisms of the annexin A1 protein and its mimetic peptide Ac2-26 in models of ocular inflammation in vivo and in vitro. J Immunol. 2013;190:5689-701 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Annexin A1 Polyclonal Antibody

Catalog # :

71-3400

Quantity :

100µL

Price :

US 309.00

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Applications :

ELISA: 1:500-1:50,000, Immunocytochemistry: 1 µg/mL, Immunofluorescence: 1 µg/mL, Immunohistochemistry (Paraffin): 1:20-1:200, Immunohistochemistry: 1:50-1:100, Western Blot: 1:100-1:1000

Species :

Human, Mouse, Rat

Isotype :

IgG

Storage :

-20°C

Description :

The Annexins are a structurally related family of calcium-dependent binding proteins that associate reversibly with membranes, and bind preferentially to the cytosolic face of phospholipid bilayers in the presence of micromolar-free calcium concentrations. This binding is specific for calcium and for acidic phospholipids. Each of the annexin proteins consists of an N-terminal domain of variable length followed by four or eight copies of a conserved segment of sixty-one residues. The repeat (sometimes known as an 'endonexin fold') consists of five alpha-helices that are wound into a right-handed superhelix. Annexins have a molecular weight of approximately 35,000 to 40,000; Annexin 1 has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins, and leukotrienes, annexin I may have potential anti-inflammatory activity. Annexins have also demonstrated to be involved in cytoskeletal interactions, intracellular signaling, anticoagulation, and membrane fusion. Although the annexins are primarily intracellular proteins and lack hydrophobic signal sequences, some family members (e.g., Annexin I and V) have been found to be secreted. Annexin I (also known as calpactin II, chromobindin 9, GIF, Lipocortin I, and p35) has been shown to be a mediator of corticosteroid activity, a substrate for serine/threonine kinases and growth-regulated tyrosine kinases, and may be involved in secretion. Although this protein has received considerable attention due to its potential involvement in a variety of pathways, research progress on the localization of Annexin I during development and in pathophysiological conditions has been hampered due to conflicting reports on the immunolocalization of the protein. These contradictory data are believed to result in large part from unusual properties of the protein and its apparent sensitivity to different fixation conditions. In addition to Annexin I, examples of other proteins known to belong to the annexin family include: .Annexin II (lipocortin 2, calpactin 1, protein I, p36, chromobindin 8), Annexin III (lipocortin 3, PAP-III), Annexin IV (lipocortin 4, endonexin I, protein II, chromobindin 4 ), Annexin V (Lipocortin 5, Endonexin 2, VAC-alpha, Anchorin CII, PAP-I), Annexin VI (Lipocortin 6, Protein III, Chromobindin 20, p68, p70), Annexin VII (Synexin), Annexin VIII (VAC-beta), Annexin XI (CAP-50), and Annexin XIII (ISA).

Immunogen :

Full-length human recombinant Annexin I protein purified from yeast.

Format :

Liquid

Applications w/Dilutions :

ELISA: 1:500-1:50,000, Immunocytochemistry: 1 µg/mL, Immunofluorescence: 1 µg/mL, Immunohistochemistry (Paraffin): 1:20-1:200, Immunohistochemistry: 1:50-1:100, Western Blot: 1:100-1:1000

Aliases :

ANXA1

PubMed References :

16633358, 18403596, 19428336, 19561612, 22539796, 23502471, 23645879, 24209638, 25174965, 25664854, 26776055, 26913609, 26935289, 27412958, 27834582, 28017544, 28062503

more info or order :

Invitrogen product webpage