NFkB p50 Polyclonal Antibody | Invitrogen 51-3500 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

NFkB p50 Polyclonal Antibody

catalog :

51-3500

quantity :

100µg

price :

US 325.00

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
western blot; rat; 1:1000; fig 8	Keshri G, Gupta A, Yadav A, Sharma S, Singh S. Photobiomodulation with Pulsed and Continuous Wave Near-Infrared Laser (810 nm, Al-Ga-As) Augments Dermal Wound Healing in Immunosuppressed Rats. PLoS ONE. 2016;11:e0166705 pubmed publisher
western blot; rat; fig 6	Gupta A, Keshri G, Yadav A, Gola S, Chauhan S, Salhan A, et al. Superpulsed (Ga-As, 904 nm) low-level laser therapy (LLLT) attenuates inflammatory response and enhances healing of burn wounds. J Biophotonics. 2015;8:489-501 pubmed publisher
western blot; human; fig 7	Campos Vega R, García Gasca T, Guevara González R, Ramos Gomez M, Oomah B, Loarca Pina G. Human gut flora-fermented nondigestible fraction from cooked bean ( Phaseolus vulgaris L.) modifies protein expression associated with apoptosis, cell cycle arrest, and proliferation in human adenocarcinoma colon cancer cells. J Agric Food Chem. 2012;60:12443-50 pubmed publisher
	Guerrero C, Paula Pardo V, Rafael Guerrero O. Inhibition of rotavirus ECwt infection in ICR suckling mice by N-acetylcysteine, peroxisome proliferator-activated receptor gamma agonists and cyclooxygenase-2 inhibitors. Mem Inst Oswaldo Cruz. 2013;108:741-54 pubmed publisher
	Bolt G, Berg K, Blixenkrone Møller M. Measles virus-induced modulation of host-cell gene expression. J Gen Virol. 2002;83:1157-65 pubmed
	Ducoux M, Urbach S, Baldacci G, Hubscher U, Koundrioukoff S, Christensen J, et al. Mediation of proliferating cell nuclear antigen (PCNA)-dependent DNA replication through a conserved p21(Cip1)-like PCNA-binding motif present in the third subunit of human DNA polymerase delta. J Biol Chem. 2001;276:49258-66 pubmed

product information

Product Type :

Antibody

Product Name :

NFkB p50 Polyclonal Antibody

Catalog # :

51-3500

Quantity :

100µg

Price :

US 325.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse

Applications :

ELISA: 0.1-1.0 µg/mL, Gel Shift: 5-10 µg/mL, Immunocytochemistry: 1 µg/mL, Immunofluorescence: 1 µg/mL, Immunohistochemistry (Paraffin): 1:20-1:200, Western Blot: 1.0 µg/mL

Species :

Human, Mouse

Isotype :

IgG

Storage :

-20°C

Description :

NFkB is a nuclear transcription factor activated by various extra and intracellular stimuli such as cytokines, UV radiation, stress, in injury, and by bacterial and viral products. NFkB is involved in regulation of various cellular events including cell growth, differentiation, proliferation, apoptosis and inflammation. NFKB1 (p50) is a 50KDa functional sub-unit of NF-kB and a member of Rel protein family which is synthesized as a p105 precursor protein and consists of an N-terminal conserved RHD-region containing nuclear localization signal, DNA-binding and dimerization domains. NFKB1 (p50) forms homodimers or heterodimerizes with p65, forming the functional NFkB factor. Further, NFKB1 (p50) directs the nuclear translocation of NF-kB and is instrumental in its DNA-binding. The NFkB complex is expressed in most cell types, and is primarily found in the cytoplasm in an inactive state in association with I-kappa-B. Phosphorylation of I-kappa-B releases the complex, allowing translocation to the nucleus and modification of gene expression. Phosphorylation of the p50 subunit at the Ser-337 residue has been shown to significantly increase DNA binding efficiency of the NFkB protein complex. The pathological role of NFkB has been suggested in AIDS, hematogenic cancer cell metastasis and rheumatoid arthritis.

Immunogen :

Recombinant protein derived from an internal region of the human NF-kappa-B (p50) (nuclear factor of kappa-B).

Format :

Liquid

Applications w/Dilutions :

ELISA: 0.1-1.0 µg/mL, Gel Shift: 5-10 µg/mL, Immunocytochemistry: 1 µg/mL, Immunofluorescence: 1 µg/mL, Immunohistochemistry (Paraffin): 1:20-1:200, Western Blot: 1.0 µg/mL

Aliases :

DNA-binding factor KBF1, EBP-1, NF-kappa-B1 p84/NF-kappa-B1 p98, NFKB1, NFkB-p50, Nuclear factor NF-kappa-B p105 subunit, nuclear factor NF-kappa-B p50 subunit

more info or order :

Invitrogen product webpage