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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
SMAD3 Polyclonal Antibody
catalog :
51-1500
quantity :
100µg
price :
US 309.00
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
hamsters, human, mouse, rat, dog
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 61
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse; 1:250; loading ...; fig 4a
  • western blot; mouse; loading ...; fig 4b
Chowdhury A, Hasselbach L, Echtermeyer F, Jyotsana N, Theilmeier G, Herzog C. Fibulin-6 regulates pro-fibrotic TGF-β responses in neonatal mouse ventricular cardiac fibroblasts. Sci Rep. 2017;7:42725 pubmed publisher
  • western blot; rat; 1:1000; loading ...; fig 7b
Zhu Y, Takayama T, Wang B, Kent A, Zhang M, Binder B, et al. Restenosis Inhibition and Re-differentiation of TGFβ/Smad3-activated Smooth Muscle Cells by Resveratrol. Sci Rep. 2017;7:41916 pubmed publisher
  • western blot; human; loading ...; fig 3f
Lefort K, Ostano P, Mello Grand M, Calpini V, Scatolini M, Farsetti A, et al. Dual tumor suppressing and promoting function of Notch1 signaling in human prostate cancer. Oncotarget. 2016;7:48011-48026 pubmed publisher
  • western blot; mouse
Strand D, Liang Y, Yang F, Barron D, Ressler S, Schauer I, et al. TGF-β induction of FGF-2 expression in stromal cells requires integrated smad3 and MAPK pathways. Am J Clin Exp Urol. 2014;2:239-48 pubmed
  • immunohistochemistry; human
Marino F, Risbridger G, Gold E. The inhibin/activin signalling pathway in human gonadal and adrenal cancers. Mol Hum Reprod. 2014;20:1223-37 pubmed publisher
  • immunohistochemistry; human; 1:400; fig 4
  • western blot; human
Anttonen M, Pihlajoki M, Andersson N, Georges A, L hôte D, Vattulainen S, et al. FOXL2, GATA4, and SMAD3 co-operatively modulate gene expression, cell viability and apoptosis in ovarian granulosa cell tumor cells. PLoS ONE. 2014;9:e85545 pubmed publisher
  • immunocytochemistry; human; 1:100
Kiriyama S, Yokoyama S, Ueno M, Hayami S, Ieda J, Yamamoto N, et al. CEACAM1 long cytoplasmic domain isoform is associated with invasion and recurrence of hepatocellular carcinoma. Ann Surg Oncol. 2014;21 Suppl 4:S505-14 pubmed publisher
  • immunocytochemistry; human
Borgogna C, Lanfredini S, Peretti A, de Andrea M, Zavattaro E, Colombo E, et al. Improved detection reveals active ?-papillomavirus infection in skin lesions from kidney transplant recipients. Mod Pathol. 2014;27:1101-15 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100
Parviainen H, Schrade A, Kiiveri S, Prunskaite Hyyryläinen R, Haglund C, Vainio S, et al. Expression of Wnt and TGF-? pathway components and key adrenal transcription factors in adrenocortical tumors: association to carcinoma aggressiveness. Pathol Res Pract. 2013;209:503-9 pubmed publisher
  • western blot; mouse; fig 4
Gao Y, Lui W. Transforming growth factor-?1 (TGF-?1) regulates cell junction restructuring via Smad-mediated repression and clathrin-mediated endocytosis of nectin-like molecule 2 (Necl-2). PLoS ONE. 2013;8:e64316 pubmed publisher
  • western blot; human; 1:1000; fig 4
Patrick A, Cabrera J, Smith A, Chen X, Ford H, Zhao R. Structure-function analyses of the human SIX1-EYA2 complex reveal insights into metastasis and BOR syndrome. Nat Struct Mol Biol. 2013;20:447-53 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:200; fig 2
Xie W, Aisner S, Baredes S, Sreepada G, Shah R, Reiss M. Alterations of Smad expression and activation in defining 2 subtypes of human head and neck squamous cell carcinoma. Head Neck. 2013;35:76-85 pubmed publisher
  • western blot; mouse; fig 1
Hong M, Wilkes M, Penheiter S, Gupta S, Edens M, Leof E. Non-Smad transforming growth factor-? signaling regulated by focal adhesion kinase binding the p85 subunit of phosphatidylinositol 3-kinase. J Biol Chem. 2011;286:17841-50 pubmed publisher
  • western blot; human; fig 3
Chen G, Ghosh P, Longo D. Distinctive mechanism for sustained TGF-β signaling and growth inhibition: MEK1 activation-dependent stabilization of type II TGF-β receptors. Mol Cancer Res. 2011;9:78-89 pubmed publisher
  • immunocytochemistry; human; fig 3
  • western blot; human; fig 2
Li Y, Cao H, Jiao Z, Pakala S, Sirigiri D, Li W, et al. Carcinoembryonic antigen interacts with TGF-{beta} receptor and inhibits TGF-{beta} signaling in colorectal cancers. Cancer Res. 2010;70:8159-68 pubmed publisher
  • western blot; dog; fig 4
Moyano J, Greciano P, Buschmann M, Koch M, Matlin K. Autocrine transforming growth factor-{beta}1 activation mediated by integrin {alpha}V{beta}3 regulates transcriptional expression of laminin-332 in Madin-Darby canine kidney epithelial cells. Mol Biol Cell. 2010;21:3654-68 pubmed publisher
  • western blot; human; fig 2
He W, Tan R, Dai C, Li Y, Wang D, Hao S, et al. Plasminogen activator inhibitor-1 is a transcriptional target of the canonical pathway of Wnt/beta-catenin signaling. J Biol Chem. 2010;285:24665-75 pubmed publisher
  • western blot; rat; 1:200; fig 4
Wang Q, Tao Y, Yuan J, Shen L, Liu C. Salvianolic acid B prevents epithelial-to-mesenchymal transition through the TGF-beta1 signal transduction pathway in vivo and in vitro. BMC Cell Biol. 2010;11:31 pubmed publisher
  • chromatin immunoprecipitation; human; fig 2
  • immunoprecipitation; human; fig 2
Bergstrom R, Savary K, Morén A, Guibert S, Heldin C, Ohlsson R, et al. Transforming growth factor beta promotes complexes between Smad proteins and the CCCTC-binding factor on the H19 imprinting control region chromatin. J Biol Chem. 2010;285:19727-37 pubmed publisher
  • western blot; human; fig 2
Lin Q, Chen D, Timchenko N, Medrano E. SKI promotes Smad3 linker phosphorylations associated with the tumor-promoting trait of TGFbeta. Cell Cycle. 2010;9:1684-9 pubmed
  • western blot; hamsters; fig 4b
Fortin J, Bernard D. SMAD3 and EGR1 physically and functionally interact in promoter-specific fashion. Cell Signal. 2010;22:936-43 pubmed publisher
  • western blot; human; fig 4
Garamszegi N, Garamszegi S, Samavarchi Tehrani P, Walford E, Schneiderbauer M, Wrana J, et al. Extracellular matrix-induced transforming growth factor-beta receptor signaling dynamics. Oncogene. 2010;29:2368-80 pubmed publisher
  • EMSA; human; fig 5
Luo D, Phillips A, Fraser D. Bone morphogenetic protein-7 inhibits proximal tubular epithelial cell Smad3 signaling via increased SnoN expression. Am J Pathol. 2010;176:1139-47 pubmed publisher
  • western blot; human; fig 1
Fukasawa H, Yamamoto T, Fujigaki Y, Misaki T, Ohashi N, Takayama T, et al. Reduction of transforming growth factor-beta type II receptor is caused by the enhanced ubiquitin-dependent degradation in human renal cell carcinoma. Int J Cancer. 2010;127:1517-25 pubmed publisher
  • immunocytochemistry; human; 1:200; fig 6
  • western blot; human; 1:200; fig 6
Wang Q, Yuan J, Tao Y, Zhang Y, Liu P, Liu C. Fuzheng Huayu recipe and vitamin E reverse renal interstitial fibrosis through counteracting TGF-beta1-induced epithelial-to-mesenchymal transition. J Ethnopharmacol. 2010;127:631-40 pubmed publisher
  • western blot; rat
Giampieri S, Manning C, Hooper S, Jones L, Hill C, Sahai E. Localized and reversible TGFbeta signalling switches breast cancer cells from cohesive to single cell motility. Nat Cell Biol. 2009;11:1287-96 pubmed publisher
  • western blot; human
Luo D, Fielding C, Phillips A, Fraser D. Interleukin-1 beta regulates proximal tubular cell transforming growth factor beta-1 signalling. Nephrol Dial Transplant. 2009;24:2655-65 pubmed publisher
  • western blot; mouse; fig 4
Wilkes M, Repellin C, Hong M, Bracamonte M, Penheiter S, Borg J, et al. Erbin and the NF2 tumor suppressor Merlin cooperatively regulate cell-type-specific activation of PAK2 by TGF-beta. Dev Cell. 2009;16:433-44 pubmed publisher
  • chromatin immunoprecipitation; mouse; fig 4
  • western blot; mouse; fig 4
  • chromatin immunoprecipitation; human; fig 4
  • western blot; human; fig 4
Smith A, Verrecchia A, Fagà G, Doni M, Perna D, Martinato F, et al. A positive role for Myc in TGFbeta-induced Snail transcription and epithelial-to-mesenchymal transition. Oncogene. 2009;28:422-30 pubmed publisher
Brandsma C, Timens W, Jonker M, Rutgers B, Noordhoek J, Postma D. Differential effects of fluticasone on extracellular matrix production by airway and parenchymal fibroblasts in severe COPD. Am J Physiol Lung Cell Mol Physiol. 2013;305:L582-9 pubmed publisher
Chai Z, Dai A, Tu Y, Li J, Wu T, Wang Y, et al. Genetic deletion of cell division autoantigen 1 retards diabetes-associated renal injury. J Am Soc Nephrol. 2013;24:1782-92 pubmed publisher
Jin Q, Gao G, Mulder K. A dynein motor attachment complex regulates TGFß/Smad3 signaling. Int J Biol Sci. 2013;9:531-40 pubmed publisher
Pandey J, Umphress S, Kang Y, Angdisen J, Naumova A, Mercer K, et al. Modulation of tumor induction and progression of oncogenic K-ras-positive tumors in the presence of TGF- b1 haploinsufficiency. Carcinogenesis. 2007;28:2589-96 pubmed
Weng H, Ciuclan L, Liu Y, Hamzavi J, Godoy P, Gaitantzi H, et al. Profibrogenic transforming growth factor-beta/activin receptor-like kinase 5 signaling via connective tissue growth factor expression in hepatocytes. Hepatology. 2007;46:1257-70 pubmed
Wu Y, Zhang X, Salmon M, Lin X, Zehner Z. TGFbeta1 regulation of vimentin gene expression during differentiation of the C2C12 skeletal myogenic cell line requires Smads, AP-1 and Sp1 family members. Biochim Biophys Acta. 2007;1773:427-39 pubmed
Liu G, Ding W, Neiman J, Mulder K. Requirement of Smad3 and CREB-1 in mediating transforming growth factor-beta (TGF beta) induction of TGF beta 3 secretion. J Biol Chem. 2006;281:29479-90 pubmed
Wilkes M, Leof E. Transforming growth factor beta activation of c-Abl is independent of receptor internalization and regulated by phosphatidylinositol 3-kinase and PAK2 in mesenchymal cultures. J Biol Chem. 2006;281:27846-54 pubmed
Kurisaki A, Kurisaki K, Kowanetz M, Sugino H, Yoneda Y, Heldin C, et al. The mechanism of nuclear export of Smad3 involves exportin 4 and Ran. Mol Cell Biol. 2006;26:1318-32 pubmed
Wohlfert E, Gorelik L, Mittler R, Flavell R, Clark R. Cutting edge: deficiency in the E3 ubiquitin ligase Cbl-b results in a multifunctional defect in T cell TGF-beta sensitivity in vitro and in vivo. J Immunol. 2006;176:1316-20 pubmed
Burdette J, Jeruss J, Kurley S, Lee E, Woodruff T. Activin A mediates growth inhibition and cell cycle arrest through Smads in human breast cancer cells. Cancer Res. 2005;65:7968-75 pubmed
Izumi N, Mizuguchi S, Inagaki Y, Saika S, Kawada N, Nakajima Y, et al. BMP-7 opposes TGF-beta1-mediated collagen induction in mouse pulmonary myofibroblasts through Id2. Am J Physiol Lung Cell Mol Physiol. 2006;290:L120-6 pubmed
Alliston T, Ko T, Cao Y, Liang Y, Feng X, Chang C, et al. Repression of bone morphogenetic protein and activin-inducible transcription by Evi-1. J Biol Chem. 2005;280:24227-37 pubmed
Boyer Arnold N, Korc M. Smad7 abrogates transforming growth factor-beta1-mediated growth inhibition in COLO-357 cells through functional inactivation of the retinoblastoma protein. J Biol Chem. 2005;280:21858-66 pubmed
Romero Gallo J, Sozmen E, Chytil A, Russell W, Whitehead R, Parks W, et al. Inactivation of TGF-beta signaling in hepatocytes results in an increased proliferative response after partial hepatectomy. Oncogene. 2005;24:3028-41 pubmed
Quan T, He T, Voorhees J, Fisher G. Ultraviolet irradiation induces Smad7 via induction of transcription factor AP-1 in human skin fibroblasts. J Biol Chem. 2005;280:8079-85 pubmed
Daniels C, Wilkes M, Edens M, Kottom T, Murphy S, Limper A, et al. Imatinib mesylate inhibits the profibrogenic activity of TGF-beta and prevents bleomycin-mediated lung fibrosis. J Clin Invest. 2004;114:1308-16 pubmed
Wolfraim L, Fernandez T, Mamura M, Fuller W, Kumar R, Cole D, et al. Loss of Smad3 in acute T-cell lymphoblastic leukemia. N Engl J Med. 2004;351:552-9 pubmed
Schneiderbauer M, Dutton C, Scully S. Signaling "cross-talk" between TGF-beta1 and ECM signals in chondrocytic cells. Cell Signal. 2004;16:1133-40 pubmed
Tian F, Byfield S, Parks W, Stuelten C, Nemani D, Zhang Y, et al. Smad-binding defective mutant of transforming growth factor beta type I receptor enhances tumorigenesis but suppresses metastasis of breast cancer cell lines. Cancer Res. 2004;64:4523-30 pubmed
Park S, Eom Y, Kim E, Lee J, Min D, Kim S, et al. Involvement of c-Src kinase in the regulation of TGF-beta1-induced apoptosis. Oncogene. 2004;23:6272-81 pubmed
Brown K, Aakre M, Gorska A, Price J, Eltom S, Pietenpol J, et al. Induction by transforming growth factor-beta1 of epithelial to mesenchymal transition is a rare event in vitro. Breast Cancer Res. 2004;6:R215-31 pubmed
Murphy S, Doré J, Edens M, Coffey R, Barnard J, Mitchell H, et al. Differential trafficking of transforming growth factor-beta receptors and ligand in polarized epithelial cells. Mol Biol Cell. 2004;15:2853-62 pubmed
Frederick J, Liberati N, Waddell D, Shi Y, Wang X. Transforming growth factor beta-mediated transcriptional repression of c-myc is dependent on direct binding of Smad3 to a novel repressive Smad binding element. Mol Cell Biol. 2004;24:2546-59 pubmed
Zavadil J, Cermak L, Soto Nieves N, Bottinger E. Integration of TGF-beta/Smad and Jagged1/Notch signalling in epithelial-to-mesenchymal transition. EMBO J. 2004;23:1155-65 pubmed
Tian F, DaCosta Byfield S, Parks W, Yoo S, Felici A, Tang B, et al. Reduction in Smad2/3 signaling enhances tumorigenesis but suppresses metastasis of breast cancer cell lines. Cancer Res. 2003;63:8284-92 pubmed
Wilkes M, Murphy S, Garamszegi N, Leof E. Cell-type-specific activation of PAK2 by transforming growth factor beta independent of Smad2 and Smad3. Mol Cell Biol. 2003;23:8878-89 pubmed
Greene R, Nugent P, Mukhopadhyay P, Warner D, Pisano M. Intracellular dynamics of Smad-mediated TGFbeta signaling. J Cell Physiol. 2003;197:261-71 pubmed
Law B, Chytil A, Dumont N, Hamilton E, Waltner Law M, Aakre M, et al. Rapamycin potentiates transforming growth factor beta-induced growth arrest in nontransformed, oncogene-transformed, and human cancer cells. Mol Cell Biol. 2002;22:8184-98 pubmed
Zhang H, Akman H, Smith E, Zhao J, Murphy Ullrich J, Batuman O. Cellular response to hypoxia involves signaling via Smad proteins. Blood. 2003;101:2253-60 pubmed
Oxburgh L, Robertson E. Dynamic regulation of Smad expression during mesenchyme to epithelium transition in the metanephric kidney. Mech Dev. 2002;112:207-11 pubmed
Yue J, Mulder K. Requirement of Ras/MAPK pathway activation by transforming growth factor beta for transforming growth factor beta 1 production in a Smad-dependent pathway. J Biol Chem. 2000;275:30765-73 pubmed
product information
Product Type :
Antibody
Product Name :
SMAD3 Polyclonal Antibody
Catalog # :
51-1500
Quantity :
100µg
Price :
US 309.00
Clonality :
Polyclonal
Purity :
Antigen affinity chromatography
Host :
Rabbit
Reactivity :
Human
Applications :
ChIP assay: 2 µg/10^6 cells, ELISA: 0.1-1.0 µg/mL, Immunocytochemistry: 1:100-1:500, Immunofluorescence: 1:100-1:500, Immunohistochemistry: 1.0 µg/mL, Western Blot: 1-3 µg/mL
Species :
Human
Isotype :
IgG
Storage :
-20°C
Description :
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
Immunogen :
A 20 amino acid synthetic peptide derived from a central portion of the linker domain of human Smad3
Format :
Liquid
Applications w/Dilutions :
ChIP assay: 2 µg/10^6 cells, ELISA: 0.1-1.0 µg/mL, Immunocytochemistry: 1:100-1:500, Immunofluorescence: 1:100-1:500, Immunohistochemistry: 1.0 µg/mL, Western Blot: 1-3 µg/mL
Aliases :
JV15-2, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, Smad 3
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
www.thermofisher.com
800-678-5599
headquarters: USA