product summary
request information :
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon
product type :
antibody
product name :
Estrogen Receptor alpha Antibody
catalog :
49-1002
quantity :
50µg
price :
US 299.00
clonality :
monoclonal
host :
mouse
reactivity :
human
application :
western blot, immunohistochemistry
more info or order :
citations: 1
Published Application/Species/DilutionReference
  • immunohistochemistry; human; 1:3000
Peña C, Champagne F. Neonatal overexpression of estrogen receptor-α alters midbrain dopamine neuron development and reverses the effects of low maternal care in female offspring. Dev Neurobiol. 2015;75:1114-24 pubmed publisher
product information
Product Type :
Antibody
Product Name :
Estrogen Receptor alpha Antibody
Catalog # :
49-1002
Quantity :
50µg
Price :
US 299.00
Clonality :
Monoclonal
Purity :
purified
Host :
Mouse
Reactivity :
Human
Applications :
ChIP assay: 5 ug, Immunohistochemistry: Assay Dependent, Western Blot: 7 ug/ml
Species :
Human
Isotype :
IgG3
Storage :
-20° C, Avoid Freeze/Thaw Cycles
Description :
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA- binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF- kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA- binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor. Isoform 3 can bind to ERE and inhibit isoform 1.
Immunogen :
Estrogen Receptor alpha
Format :
Frozen
Applications w/Dilutions :
ChIP assay: 5 ug, Immunohistochemistry: Assay Dependent, Western Blot: 7 ug/ml
Aliases :
ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1, ESR1, ESR, NR3A1
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
www.thermofisher.com
800-678-5599
headquarters: USA