Phospho-Cortactin (Tyr421) Polyclonal Antibody | Invitrogen 44-854G product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-Cortactin (Tyr421) Polyclonal Antibody

catalog :

44-854G

quantity :

10 blots

price :

US 309.00

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Invitrogen product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; rat; fig 1g immunocytochemistry; mouse; fig 1h	Mukhopadhyay U, Eves R, Jia L, Mooney P, Mak A. p53 suppresses Src-induced podosome and rosette formation and cellular invasiveness through the upregulation of caldesmon. Mol Cell Biol. 2009;29:3088-98 pubmed publisher
immunocytochemistry; mouse; fig s2	Mukhopadhyay U, Mooney P, Jia L, Eves R, Raptis L, Mak A. Doubles game: Src-Stat3 versus p53-PTEN in cellular migration and invasion. Mol Cell Biol. 2010;30:4980-95 pubmed publisher
western blot; rat; 1:250	Lydka M, Bilinska B, Cheng C, Mruk D. Tumor necrosis factor α-mediated restructuring of the Sertoli cell barrier in vitro involves matrix metalloprotease 9 (MMP9), membrane-bound intercellular adhesion molecule-1 (ICAM-1) and the actin cytoskeleton. Spermatogenesis. 2012;2:294-303 pubmed
western blot; mouse	Sachdev S, Bu Y, Gelman I. Paxillin-Y118 phosphorylation contributes to the control of Src-induced anchorage-independent growth by FAK and adhesion. BMC Cancer. 2009;9:12 pubmed publisher
immunocytochemistry; rat	Furmaniak-Kazmierczak E, Crawley S, Carter R, Maurice D, Côté G. Formation of extracellular matrix-digesting invadopodia by primary aortic smooth muscle cells. Circ Res. 2007;100:1328-36 pubmed
	Head J, Jiang D, Li M, Zorn L, Schaefer E, Parsons J, et al. Cortactin tyrosine phosphorylation requires Rac1 activity and association with the cortical actin cytoskeleton. Mol Biol Cell. 2003;14:3216-29 pubmed
	Brabek J, Constancio S, Siesser P, Shin N, Pozzi A, Hanks S. Crk-associated substrate tyrosine phosphorylation sites are critical for invasion and metastasis of SRC-transformed cells. Mol Cancer Res. 2005;3:307-15 pubmed

product information

Product Type :

Antibody

Product Name :

Phospho-Cortactin (Tyr421) Polyclonal Antibody

Catalog # :

44-854G

Quantity :

10 blots

Price :

US 309.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse

Applications :

Immunocytochemistry: 1:250, Immunofluorescence: 1:250, Western Blot: 1:250

Species :

Human, Mouse

Isotype :

IgG

Storage :

-20°C

Description :

Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino-terminal tandem repeats of a unique 37-amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha-helical structure followed by a proline-rich region.

Immunogen :

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of mouse cortactin that contains tyrosine 421.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:250, Immunofluorescence: 1:250, Western Blot: 1:250

Aliases :

1110020L01Rik, Amplaxin, cortactin, CTTN, EMS1, ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate), FLJ34459, Oncogene EMS1, Src substrate cortactin, SRC8

PubMed References :

12925758, 15972849, 17446433, 19138410, 19349302, 20733006, 23248771

more info or order :

Invitrogen product webpage