product summary
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NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon
product type :
product name :
Estrogen Receptor alpha Antibody (H4624)
catalog :
quantity :
price :
US 455.00
clonality :
host :
reactivity :
human, rat, sheep
application :
western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry
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citations: 1
Published Application/Species/DilutionReference
  • flow cytometry; sheep; 1:10
  • immunohistochemistry; sheep; 1:50
Alexandre-Pires G, Martins C, Galvão A, Correia M, Ramilo D, Quaresma M, et al. Morphological aspects and expression of estrogen and progesterone receptors in the interdigital sinus in cyclic ewes. Microsc Res Tech. 2014;77:313-25 pubmed
product information
Product Type :
Product Name :
Estrogen Receptor alpha Antibody (H4624)
Catalog # :
Quantity :
Price :
US 455.00
Clonality :
Purity :
Ammonium sulfate precipitation
Host :
Reactivity :
Human, Rat
Applications :
ELISA: Assay Dependent, Immunohistochemistry: Assay Dependent, Immunoprecipitation: Assay Dependent, Western Blot: Assay Dependent
Species :
Human, Rat
Clone :
Isotype :
Storage :
Description :
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA- binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF- kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA- binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor. Isoform 3 can bind to ERE and inhibit isoform 1.
Immunogen :
Produced in BALB/c mouse ascites after inoculation with hybridoma of mouse myeloma cells (NS-1) and spleen cells derived from a BALB/c mouse immunized with Baculovirus-expressed recombinant human ER-alpha (2-180 aa).
Format :
Applications w/Dilutions :
ELISA: Assay Dependent, Immunohistochemistry: Assay Dependent, Immunoprecipitation: Assay Dependent, Western Blot: Assay Dependent
Aliases :
ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1, ESR1, ESR, NR3A1
PubMed References :
more info or order :
company information
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
headquarters: USA