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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
GAPDH Monoclonal Antibody (ZG003)
catalog :
39-8600
quantity :
100µg
price :
US 309.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
ZG003
reactivity :
Domestic guinea pig, human, mouse, dog, pig
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
citations: 34
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:500; fig 2h
Herold N, Rudd S, Ljungblad L, Sanjiv K, Myrberg I, Paulin C, et al. Targeting SAMHD1 with the Vpx protein to improve cytarabine therapy for hematological malignancies. Nat Med. 2017;: pubmed publisher
  • western blot; human; 1:5000; fig 1
Skrdlant L, Stark J, Lin R. Myelodysplasia-associated mutations in serine/arginine-rich splicing factor SRSF2 lead to alternative splicing of CDC25C. BMC Mol Biol. 2016;17:18 pubmed publisher
  • western blot; human
  • western blot; mouse
Zhang J, Gao Q, Zhou Y, Dier U, Hempel N, Hochwald S. Focal adhesion kinase-promoted tumor glucose metabolism is associated with a shift of mitochondrial respiration to glycolysis. Oncogene. 2016;35:1926-42 pubmed publisher
  • western blot; human; 1:1000; fig 3
Maza P, Suzuki E. Histoplasma capsulatum-Induced Cytokine Secretion in Lung Epithelial Cells Is Dependent on Host Integrins, Src-Family Kinase Activation, and Membrane Raft Recruitment. Front Microbiol. 2016;7:580 pubmed publisher
  • western blot; Domestic guinea pig; 1:1000; fig 7
Álvarez-Santos M, Carbajal V, Tellez-Jiménez O, Martínez-Cordero E, Ruiz V, Hernandez-Pando R, et al. Airway Hyperresponsiveness in Asthma Model Occurs Independently of Secretion of β1 Integrins in Airway Wall and Focal Adhesions Proteins Down Regulation. J Cell Biochem. 2016;117:2385-96 pubmed publisher
  • western blot; human; 1:10,000; fig 1a
Barros B, Maza P, Alcantara C, Suzuki E. Paracoccidioides brasiliensis induces recruitment of α3 and α5 integrins into epithelial cell membrane rafts, leading to cytokine secretion. Microbes Infect. 2016;18:68-77 pubmed publisher
  • western blot; human; fig 4
Ortuno D, Carlisle H, Miller S. Does inactivation of USP14 enhance degradation of proteasomal substrates that are associated with neurodegenerative diseases?. F1000Res. 2016;5:137 pubmed publisher
  • western blot; human; fig 5
Verma S, Mohapatra G, Ahmad S, Rana S, Jain S, Khalsa J, et al. Salmonella Engages Host MicroRNAs To Modulate SUMOylation: a New Arsenal for Intracellular Survival. Mol Cell Biol. 2015;35:2932-46 pubmed publisher
  • western blot; human; fig 3
Coudé M, Braun T, Berrou J, Dupont M, Bertrand S, Massé A, et al. BET inhibitor OTX015 targets BRD2 and BRD4 and decreases c-MYC in acute leukemia cells. Oncotarget. 2015;6:17698-712 pubmed
  • western blot; human; 1:1000; fig 2
Chesarino N, McMichael T, Yount J. E3 Ubiquitin Ligase NEDD4 Promotes Influenza Virus Infection by Decreasing Levels of the Antiviral Protein IFITM3. PLoS Pathog. 2015;11:e1005095 pubmed publisher
  • western blot; human; 1:5000
Zhao Y, Zhao L, Wang P, Miao Y, Liu Y, Wang Z, et al. Overexpression of miR-18a negatively regulates myocyte enhancer factor 2D to increase the permeability of the blood-tumor barrier via Krüppel-like factor 4-mediated downregulation of zonula occluden-1, claudin-5, and occludin. J Neurosci Res. 2015;93:1891-902 pubmed publisher
  • western blot; human; 1:10000
Stalekar M, Yin X, Rebolj K, Darovic S, Troakes C, Mayr M, et al. Proteomic analyses reveal that loss of TDP-43 affects RNA processing and intracellular transport. Neuroscience. 2015;293:157-70 pubmed publisher
  • western blot; human; fig 4
Albrecht I, Wick C, Hallgren A, Tjärnlund A, Nagaraju K, Andrade F, et al. Development of autoantibodies against muscle-specific FHL1 in severe inflammatory myopathies. J Clin Invest. 2015;125:4612-24 pubmed publisher
  • western blot; human; 1:2000; fig 6
Liikanen I, Dias J, Nokisalmi P, Sloniecka M, Kangasniemi L, Rajecki M, et al. Adenoviral E4orf3 and E4orf6 proteins, but not E1B55K, increase killing of cancer cells by radiotherapy in vivo. Int J Radiat Oncol Biol Phys. 2010;78:1201-9 pubmed publisher
  • western blot; human; 1:10000; fig s1
Shtam T, Kovalev R, Varfolomeeva E, Makarov E, Kil Y, Filatov M. Exosomes are natural carriers of exogenous siRNA to human cells in vitro. Cell Commun Signal. 2013;11:88 pubmed publisher
  • western blot; human; 1:1000; fig 6
Nguyen T, Galvan V, Huang W, Banwait S, Tang H, Zhang J, et al. Signal transduction in Alzheimer disease: p21-activated kinase signaling requires C-terminal cleavage of APP at Asp664. J Neurochem. 2008;104:1065-80 pubmed
  • western blot; mouse; 1:500
Olenich S, Audet G, Roberts K, Olfert I. Effects of detraining on the temporal expression of positive and negative angioregulatory proteins in skeletal muscle of mice. J Physiol. 2014;592:3325-38 pubmed publisher
  • western blot; human; fig 3
Shimojo M, Shudo Y, Ikeda M, Kobashi T, Ito S. The small cell lung cancer-specific isoform of RE1-silencing transcription factor (REST) is regulated by neural-specific Ser/Arg repeat-related protein of 100 kDa (nSR100). Mol Cancer Res. 2013;11:1258-68 pubmed publisher
  • western blot; human; fig 7
Calabro S, Maczurek A, Morgan A, Tu T, Wen V, Yee C, et al. Hepatocyte produced matrix metalloproteinases are regulated by CD147 in liver fibrogenesis. PLoS ONE. 2014;9:e90571 pubmed publisher
  • western blot; dog; fig 7
Kyöstilä K, Lappalainen A, Lohi H. Canine chondrodysplasia caused by a truncating mutation in collagen-binding integrin alpha subunit 10. PLoS ONE. 2013;8:e75621 pubmed publisher
  • western blot; human; 1:15,000
Spatara M, Robinson A. Transgenic mouse and cell culture models demonstrate a lack of mechanistic connection between endoplasmic reticulum stress and tau dysfunction. J Neurosci Res. 2010;88:1951-61 pubmed publisher
  • western blot; mouse; 1:1000; fig 2
Chesarino N, McMichael T, Hach J, Yount J. Phosphorylation of the antiviral protein interferon-inducible transmembrane protein 3 (IFITM3) dually regulates its endocytosis and ubiquitination. J Biol Chem. 2014;289:11986-92 pubmed publisher
  • western blot; human; fig 6
Maarouf C, Kokjohn T, Walker D, Whiteside C, Kalback W, Whetzel A, et al. Biochemical assessment of precuneus and posterior cingulate gyrus in the context of brain aging and Alzheimer's disease. PLoS ONE. 2014;9:e105784 pubmed publisher
  • western blot; human; fig 1
Fang F, Rycyzyn M, Clevenger C. Role of c-Myb during prolactin-induced signal transducer and activator of transcription 5a signaling in breast cancer cells. Endocrinology. 2009;150:1597-606 pubmed publisher
  • western blot; human; fig 3
Li L, Sarver A, Alamgir S, Subramanian S. Downregulation of microRNAs miR-1, -206 and -29 stabilizes PAX3 and CCND2 expression in rhabdomyosarcoma. Lab Invest. 2012;92:571-83 pubmed publisher
  • western blot; human; 1:5000; fig 4c
Ni Z, Xu C, Guo X, Hunter G, Kuznetsova O, Tempel W, et al. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nat Struct Mol Biol. 2014;21:686-95 pubmed publisher
  • western blot; pig; fig 6
Prunier F, Kawase Y, Gianni D, Scapin C, Danik S, Ellinor P, et al. Prevention of ventricular arrhythmias with sarcoplasmic reticulum Ca2+ ATPase pump overexpression in a porcine model of ischemia reperfusion. Circulation. 2008;118:614-24 pubmed publisher
Gemel J, Levy A, Simon A, Bennett K, Ai X, AKHTER S, et al. Connexin40 abnormalities and atrial fibrillation in the human heart. J Mol Cell Cardiol. 2014;76:159-68 pubmed publisher
Paschoud S, Yu D, Pulimeno P, Jond L, Turner J, Citi S. Cingulin and paracingulin show similar dynamic behaviour, but are recruited independently to junctions. Mol Membr Biol. 2011;28:123-35 pubmed publisher
Olenich S, Gutierrez-Reed N, Audet G, Olfert I. Temporal response of positive and negative regulators in response to acute and chronic exercise training in mice. J Physiol. 2013;591:5157-69 pubmed publisher
Leonardi R, Almeida L, Trevilatto P, Loreto C. Occurrence and regional distribution of TRAIL and DR5 on temporomandibular joint discs: comparison of disc derangement with and without reduction. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010;109:244-51 pubmed publisher
Fang F, Antico G, Zheng J, Clevenger C. Quantification of PRL/Stat5 signaling with a novel pGL4-CISH reporter. BMC Biotechnol. 2008;8:11 pubmed publisher
Wang Y, Mulligan C, Denyer G, Delom F, Dagna-Bricarelli F, Tybulewicz V, et al. Quantitative proteomics characterization of a mouse embryonic stem cell model of Down syndrome. Mol Cell Proteomics. 2009;8:585-95 pubmed publisher
White L, Declercq W, Arfuso F, Charles A, Dharmarajan A. Function of caspase-14 in trophoblast differentiation. Reprod Biol Endocrinol. 2009;7:98 pubmed publisher
product information
Product Type :
Antibody
Product Name :
GAPDH Monoclonal Antibody (ZG003)
Catalog # :
39-8600
Quantity :
100µg
Price :
US 309.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Human, Mouse
Applications :
ELISA: Assay Dependent, Immunocytochemistry: 1-2 ug/ml, Immunofluorescence: 1-2 ug/ml, Immunohistochemistry (Paraffin): 1:20-1:200, Western Blot: 0.5-2 ug/ml
Species :
Human, Mouse
Clone :
ZG003
Isotype :
IgG1, kappa
Storage :
-20°C
Description :
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is an enzyme involved in glycolysis. In addition to this role, it is involved in other cellular processes ranging from membrane fusion, neuronal apoptosis to cancer. GAPDH is expressed at high levels in most tissues and is therefore useful as protein loading control in western blot analysis. Additionally, the antigen is conserved in most eukaryotes.
Immunogen :
Recombinant full-length human GAPDH protein
Format :
Liquid
Applications w/Dilutions :
ELISA: Assay Dependent, Immunocytochemistry: 1-2 ug/ml, Immunofluorescence: 1-2 ug/ml, Immunohistochemistry (Paraffin): 1:20-1:200, Western Blot: 0.5-2 ug/ml
Aliases :
Glyceraldehyde-3-Phosphate Dehydrogenase, GAPD, G3PD, G3PDH
PubMed References :
17986220, 18254957, 18645052, 19001410, 19036881, 19747408, 20123409, 20143409, 20832189, 21166484, 22330340, 23878369, 23928058, 24086591, 24245560, 24627473, 24951625, 24997600, 25076423, 25166759, 25200600, 25743254, 25989842, 26100020, 26119934, 26263374, 26356851, 26369712, 26551678, 26969873, 26998235, 27148251, 27552991, 28067901
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
www.thermofisher.com
800-678-5599
headquarters: USA