product summary
request information :
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon
product type :
antibody
product name :
GAPDH Antibody (ZG003)
catalog :
39-8600
quantity :
100µg
price :
US 299.00
clonality :
monoclonal
host :
mouse
reactivity :
human, mouse, dog
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
citations: 14
Published Application/Species/DilutionReference
  • western blot; human
  • western blot; mouse
Zhang J, Gao Q, Zhou Y, Dier U, Hempel N, Hochwald S. Focal adhesion kinase-promoted tumor glucose metabolism is associated with a shift of mitochondrial respiration to glycolysis. Oncogene. 2015;: pubmed publisher
  • western blot; human; 1:10000
Stalekar M, Yin X, Rebolj K, Darovic S, Troakes C, Mayr M, et al. Proteomic analyses reveal that loss of TDP-43 affects RNA processing and intracellular transport. Neuroscience. 2015;293:157-70 pubmed publisher
  • western blot; human; 1:5000
Zhao Y, Zhao L, Wang P, Miao Y, Liu Y, Wang Z, et al. Overexpression of miR-18a negatively regulates myocyte enhancer factor 2D to increase the permeability of the blood-tumor barrier via Krüppel-like factor 4-mediated downregulation of zonula occluden-1, claudin-5, and occludin. J Neurosci Res. 2015;93:1891-902 pubmed publisher
  • western blot; human; fig 5
Verma S, Mohapatra G, Ahmad S, Rana S, Jain S, Khalsa J, et al. Salmonella Engages Host MicroRNAs To Modulate SUMOylation: a New Arsenal for Intracellular Survival. Mol Cell Biol. 2015;35:2932-46 pubmed publisher
  • western blot; human; fig 7
Calabro S, Maczurek A, Morgan A, Tu T, Wen V, Yee C, et al. Hepatocyte produced matrix metalloproteinases are regulated by CD147 in liver fibrogenesis. PLoS ONE. 2014;9:e90571 pubmed publisher
  • western blot; human; 1:5000; fig 4c
Ni Z, Xu C, Guo X, Hunter G, Kuznetsova O, Tempel W, et al. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nat Struct Mol Biol. 2014;21:686-95 pubmed publisher
  • western blot; mouse; 1:1000; fig 2
Chesarino N, McMichael T, Hach J, Yount J. Phosphorylation of the antiviral protein interferon-inducible transmembrane protein 3 (IFITM3) dually regulates its endocytosis and ubiquitination. J Biol Chem. 2014;289:11986-92 pubmed publisher
  • western blot; mouse; 1:500
Olenich S, Audet G, Roberts K, Olfert I. Effects of detraining on the temporal expression of positive and negative angioregulatory proteins in skeletal muscle of mice. J Physiol. 2014;592:3325-38 pubmed publisher
  • western blot; human; fig 3
Shimojo M, Shudo Y, Ikeda M, Kobashi T, Ito S. The small cell lung cancer-specific isoform of RE1-silencing transcription factor (REST) is regulated by neural-specific Ser/Arg repeat-related protein of 100 kDa (nSR100). Mol Cancer Res. 2013;11:1258-68 pubmed publisher
  • western blot; human; 1:10000; fig s1
Shtam T, Kovalev R, Varfolomeeva E, Makarov E, Kil Y, Filatov M. Exosomes are natural carriers of exogenous siRNA to human cells in vitro. Cell Commun Signal. 2013;11:88 pubmed publisher
  • western blot; dog; fig 7
Kyöstilä K, Lappalainen A, Lohi H. Canine chondrodysplasia caused by a truncating mutation in collagen-binding integrin alpha subunit 10. PLoS ONE. 2013;8:e75621 pubmed publisher
  • western blot; human; 1:1000; fig 6
Nguyen T, Galvan V, Huang W, Banwait S, Tang H, Zhang J, et al. Signal transduction in Alzheimer disease: p21-activated kinase signaling requires C-terminal cleavage of APP at Asp664. J Neurochem. 2008;104:1065-80 pubmed
  • western blot; human; fig 3
Li L, Sarver A, Alamgir S, Subramanian S. Downregulation of microRNAs miR-1, -206 and -29 stabilizes PAX3 and CCND2 expression in rhabdomyosarcoma. Lab Invest. 2012;92:571-83 pubmed publisher
Fang F, Rycyzyn M, Clevenger C. Role of c-Myb during prolactin-induced signal transducer and activator of transcription 5a signaling in breast cancer cells. Endocrinology. 2009;150:1597-606 pubmed publisher
product information
Product Type :
Antibody
Product Name :
GAPDH Antibody (ZG003)
Catalog # :
39-8600
Quantity :
100µg
Price :
US 299.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Human, Mouse
Applications :
ELISA: Assay Dependent, Immunocytochemistry: 1-2 ug/ml, Immunofluorescence: 1-2 ug/ml, Immunohistochemistry (Paraffin): 1:20-1:200, Western Blot: 0.5-2 ug/ml
Species :
Human, Mouse
Clone :
ZG003
Isotype :
IgG1, kappa
Storage :
-20°C
Description :
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is an enzyme involved in glycolysis. In addition to this role, it is involved in other cellular processes ranging from membrane fusion, neuronal apoptosis to cancer. GAPDH is expressed at high levels in most tissues and is therefore useful as protein loading control in western blot analysis. Additionally, the antigen is conserved in most eukaryotes.
Immunogen :
Recombinant full-length human GAPDH protein
Format :
Liquid
Applications w/Dilutions :
ELISA: Assay Dependent, Immunocytochemistry: 1-2 ug/ml, Immunofluorescence: 1-2 ug/ml, Immunohistochemistry (Paraffin): 1:20-1:200, Western Blot: 0.5-2 ug/ml
Aliases :
Glyceraldehyde-3-Phosphate Dehydrogenase, GAPD, G3PD, G3PDH
PubMed References :
24951625, 25076423, 25743254
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
www.thermofisher.com
800-678-5599
headquarters: USA