PTBP1 Monoclonal Antibody (1) | Invitrogen 32-4800 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

PTBP1 Monoclonal Antibody (1)

catalog :

32-4800

quantity :

100µg

price :

US 309.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, RNA immunoprecipitation, western blot knockout validation

more info or order :

Invitrogen product webpage

citations: 18

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; mouse; loading ...; fig 1c	Jin Z, Liang F, Yang J, Mei W. hnRNP I regulates neonatal immune adaptation and prevents colitis and colorectal cancer. PLoS Genet. 2017;13:e1006672 pubmed publisher
RNA immunoprecipitation; human; loading ...; fig 1d western blot; human; loading ...; fig 1c	Zhang L, Yang Z, Trottier J, Barbier O, Wang L. Long noncoding RNA MEG3 induces cholestatic liver injury by interaction with PTBP1 to facilitate shp mRNA decay. Hepatology. 2017;65:604-615 pubmed publisher
western blot; rat; fig 4 western blot; mouse; fig 7	Llorian M, Gooding C, Bellora N, Hallegger M, Buckroyd A, Wang X, et al. The alternative splicing program of differentiated smooth muscle cells involves concerted non-productive splicing of post-transcriptional regulators. Nucleic Acids Res. 2016;44:8933-8950 pubmed
western blot; human; loading ...; fig 3e	Tang S, Luo S, Ho J, Ly P, Goh E, Roca X. Characterization of the Regulation of CD46 RNA Alternative Splicing. J Biol Chem. 2016;291:14311-23 pubmed publisher
RNA immunoprecipitation; human; fig 1	Marnef A, JÃ¡dy B, Kiss T. Human polypyrimidine tract-binding protein interacts with mitochondrial tRNA(Thr) in the cytosol. Nucleic Acids Res. 2016;44:1342-53 pubmed publisher
western blot; human	Panas M, Kedersha N, McInerney G. Methods for the characterization of stress granules in virus infected cells. Methods. 2015;90:57-64 pubmed publisher
western blot; human; 1:1000; fig 5	Marzese D, Liu M, Huynh J, Hirose H, Donovan N, Huynh K, et al. Brain metastasis is predetermined in early stages of cutaneous melanoma by CD44v6 expression through epigenetic regulation of the spliceosome. Pigment Cell Melanoma Res. 2015;28:82-93 pubmed publisher
western blot; human; fig 6	Ye J, Cardona M, Llovera M, Comella J, Sanchis D. Translation of Myocyte Enhancer Factor-2 is induced by hypertrophic stimuli in cardiomyocytes through a Calcineurin-dependent pathway. J Mol Cell Cardiol. 2012;53:578-87 pubmed publisher
western blot; mouse; 1:2000; fig 2	Shibasaki T, Tokunaga A, Sakamoto R, Sagara H, Noguchi S, Sasaoka T, et al. PTB deficiency causes the loss of adherens junctions in the dorsal telencephalon and leads to lethal hydrocephalus. Cereb Cortex. 2013;23:1824-35 pubmed publisher
western blot; mouse; fig 4	Smith P, Delay C, Girard J, Papon M, Planel E, Sergeant N, et al. MicroRNA-132 loss is associated with tau exon 10 inclusion in progressive supranuclear palsy. Hum Mol Genet. 2011;20:4016-24 pubmed publisher
immunocytochemistry; human; 1:50; fig 5	Borghese F, Michiels T. The leader protein of cardioviruses inhibits stress granule assembly. J Virol. 2011;85:9614-22 pubmed publisher
immunohistochemistry; mouse; fig 2	Suckale J, Wendling O, Masjkur J, Jäger M, Münster C, Anastassiadis K, et al. PTBP1 is required for embryonic development before gastrulation. PLoS ONE. 2011;6:e16992 pubmed publisher
western blot; mouse; fig 2	Smith P, Al Hashimi A, Girard J, Delay C, Hébert S. In vivo regulation of amyloid precursor protein neuronal splicing by microRNAs. J Neurochem. 2011;116:240-7 pubmed publisher
immunocytochemistry; mouse; 1:50; fig 4	Paul S, Michiels T. Cardiovirus leader proteins are functionally interchangeable and have evolved to adapt to virus replication fitness. J Gen Virol. 2006;87:1237-46 pubmed
western blot; human; 1:250; fig 2	Adair R, Liebisch G, Su Y, COLBERG POLEY A. Alteration of cellular RNA splicing and polyadenylation machineries during productive human cytomegalovirus infection. J Gen Virol. 2004;85:3541-53 pubmed
	Pradeepa M, Sutherland H, Ule J, Grimes G, Bickmore W. Psip1/Ledgf p52 binds methylated histone H3K36 and splicing factors and contributes to the regulation of alternative splicing. PLoS Genet. 2012;8:e1002717 pubmed publisher
	Delhaye S, van Pesch V, Michiels T. The leader protein of Theiler's virus interferes with nucleocytoplasmic trafficking of cellular proteins. J Virol. 2004;78:4357-62 pubmed
	Knoch K, Bergert H, Borgonovo B, Saeger H, Altkr ger A, Verkade P, et al. Polypyrimidine tract-binding protein promotes insulin secretory granule biogenesis. Nat Cell Biol. 2004;6:207-14 pubmed publisher

product information

Product Type :

Antibody

Product Name :

PTBP1 Monoclonal Antibody (1)

Catalog # :

32-4800

Quantity :

100µg

Price :

US 309.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

Flow Cytometry: 3-5 µg/1x10^6 cells, Immunocytochemistry: 2 µg/mL, Immunofluorescence: 2 µg/mL, Immunoprecipitation: Assay Dependent, Western Blot: 1-3 µg/mL

Species :

Human, Mouse, Rat

Clone :

Isotype :

IgG1

Storage :

-20°C

Description :

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein, PTB (polypyrimidine tract binding protein; PTBP1), encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar compartment (PNC), a structure localized at the periphery of the nucleolus. Thus far PTB as well as small RNAs (i.e, Y RNAs, MRP RNA, and Rnase P H1 RNA) have been identified in the PNC. Studies conducted to characterize the structure of the PNC, revealed that RNA binding may be required for PTB to be localized in the PNC. Alternatively spliced transcript variants encoding different isoforms have been described.

Immunogen :

Recombinant human PTB protein.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 3-5 µg/1x10^6 cells, Immunocytochemistry: 2 µg/mL, Immunofluorescence: 2 µg/mL, Immunoprecipitation: Assay Dependent, Western Blot: 1-3 µg/mL

Aliases :

HNRNP-I, HNRNPI, HNRPI, PTB, PTB-1, PTB-T, PTB2, PTB3, PTB4, pPTB, 57 kDa RNA-binding protein PPTB-1, RNA-binding protein, heterogeneous nuclear ribonucleoprotein I, heterogeneous nuclear ribonucleoprotein polypeptide I, hnRNP I, polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I), polypyrimidine t

more info or order :

Invitrogen product webpage