alpha Tubulin Monoclonal Antibody (TU-01) | Invitrogen 13-8000 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

alpha Tubulin Monoclonal Antibody (TU-01)

catalog :

13-8000

quantity :

100µg

price :

US 299.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

TU-01

reactivity :

African green monkey, human, mouse, rat, common platanna

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 34

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:400 western blot; mouse; 1:400	Sasaki M, Yoshimura Miyakoshi M, Sato Y, Nakanuma Y. A possible involvement of endoplasmic reticulum stress in biliary epithelial autophagy and senescence in primary biliary cirrhosis. J Gastroenterol. 2015;50:984-95 pubmed publisher
western blot; human	Hashimoto Y, Shirane M, Matsuzaki F, Saita S, Ohnishi T, Nakayama K. Protrudin regulates endoplasmic reticulum morphology and function associated with the pathogenesis of hereditary spastic paraplegia. J Biol Chem. 2014;289:12946-61 pubmed publisher
western blot; human; fig 5	Sasaki M, Miyakoshi M, Sato Y, Nakanuma Y. A possible involvement of p62/sequestosome-1 in the process of biliary epithelial autophagy and senescence in primary biliary cirrhosis. Liver Int. 2012;32:487-99 pubmed publisher
western blot; human; 1:7500; fig 2, 3	Tong J, Furukawa Y, Sherwin A, Hornykiewicz O, Kish S. Heterogeneous intrastriatal pattern of proteins regulating axon growth in normal adult human brain. Neurobiol Dis. 2011;41:458-68 pubmed publisher
immunoprecipitation; human; fig 5 immunocytochemistry; human; fig 6 western blot; human; fig 4	Tai C, Shen S, Lee W, Liao C, Deng W, Chiou H, et al. Increased cellular apoptosis susceptibility (CSE1L/CAS) protein expression promotes protrusion extension and enhances migration of MCF-7 breast cancer cells. Exp Cell Res. 2010;316:2969-81 pubmed publisher
western blot; human; fig 2	Liao C, Luo S, Tsai C, Tsao T, Chen S, Jiang M. CAS Enhances Chemotherapeutic Drug-Induced p53 Accumulation and Apoptosis: Use of CAS for High-Sensitivity Anticancer Drug Screening. Toxicol Mech Methods. 2008;18:771-6 pubmed publisher
western blot; human; fig 2	Sasaki M, Yamaguchi J, Ikeda H, Itatsu K, Nakanuma Y. Polycomb group protein Bmi1 is overexpressed and essential in anchorage-independent colony formation, cell proliferation and repression of cellular senescence in cholangiocarcinoma: tissue and culture studies. Hum Pathol. 2009;40:1723-30 pubmed publisher
immunocytochemistry; human	Miyajima N, Maruyama S, Nonomura K, Hatakeyama S. TRIM36 interacts with the kinetochore protein CENP-H and delays cell cycle progression. Biochem Biophys Res Commun. 2009;381:383-7 pubmed publisher
western blot; human	Tsao T, Tsai C, Tung J, Chen S, Yue C, Liao C, et al. Function of CSE1L/CAS in the secretion of HT-29 human colorectal cells and its expression in human colon. Mol Cell Biochem. 2009;327:163-70 pubmed publisher
western blot; human; 1 ug/ml; fig 3	Kano S, Miyajima N, Fukuda S, Hatakeyama S. Tripartite motif protein 32 facilitates cell growth and migration via degradation of Abl-interactor 2. Cancer Res. 2008;68:5572-80 pubmed publisher
western blot; human; fig 4	Sasaki M, Ikeda H, Itatsu K, Yamaguchi J, Sawada S, Minato H, et al. The overexpression of polycomb group proteins Bmi1 and EZH2 is associated with the progression and aggressive biological behavior of hepatocellular carcinoma. Lab Invest. 2008;88:873-82 pubmed publisher
immunocytochemistry; human; 1:100; fig 4	Abdulghani J, Gu L, Dagvadorj A, Lutz J, Leiby B, Bonuccelli G, et al. Stat3 promotes metastatic progression of prostate cancer. Am J Pathol. 2008;172:1717-28 pubmed publisher
western blot; human; fig 2	Omura T, Kaneko M, Onoguchi M, Koizumi S, Itami M, Ueyama M, et al. Novel functions of ubiquitin ligase HRD1 with transmembrane and proline-rich domains. J Pharmacol Sci. 2008;106:512-9 pubmed
immunohistochemistry; mouse; fig 1	Sumioka A, Saito Y, Sakuma M, Araki Y, Yamamoto T, Suzuki T. The X11L/X11beta/MINT2 and X11L2/X11gamma/MINT3 scaffold proteins shuttle between the nucleus and cytoplasm. Exp Cell Res. 2008;314:1155-62 pubmed publisher
western blot; human; fig 1	Kaneko M, Yasui S, Niinuma Y, Arai K, Omura T, Okuma Y, et al. A different pathway in the endoplasmic reticulum stress-induced expression of human HRD1 and SEL1 genes. FEBS Lett. 2007;581:5355-60 pubmed
western blot; human; 1:7500; fig 3	Tong J, Hornykiewicz O, Furukawa Y, Kish S. Marked dissociation between high noradrenaline versus low noradrenaline transporter levels in human nucleus accumbens. J Neurochem. 2007;102:1691-1702 pubmed publisher
western blot; mouse; fig 3	Li P, Waters R, Redfern S, Zhang M, Mao L, Annex B, et al. Oxidative phenotype protects myofibers from pathological insults induced by chronic heart failure in mice. Am J Pathol. 2007;170:599-608 pubmed
western blot; mouse	Nakaya T, Suzuki T. Role of APP phosphorylation in FE65-dependent gene transactivation mediated by AICD. Genes Cells. 2006;11:633-45 pubmed
western blot; mouse; fig 2	Akimoto T, Pohnert S, Li P, Zhang M, Gumbs C, Rosenberg P, et al. Exercise stimulates Pgc-1alpha transcription in skeletal muscle through activation of the p38 MAPK pathway. J Biol Chem. 2005;280:19587-93 pubmed
western blot; human; 1:1000	Trushina E, Dyer R, Badger J, Ure D, Eide L, Tran D, et al. Mutant huntingtin impairs axonal trafficking in mammalian neurons in vivo and in vitro. Mol Cell Biol. 2004;24:8195-209 pubmed
western blot; mouse	Tsunematsu R, Nakayama K, Oike Y, Nishiyama M, Ishida N, Hatakeyama S, et al. Mouse Fbw7/Sel-10/Cdc4 is required for notch degradation during vascular development. J Biol Chem. 2004;279:9417-23 pubmed
immunocytochemistry; African green monkey; fig 4	Ruddy M, Wong G, Liu X, Yamamoto H, Kasayama S, Kirkwood K, et al. Functional cooperation between interleukin-17 and tumor necrosis factor-alpha is mediated by CCAAT/enhancer-binding protein family members. J Biol Chem. 2004;279:2559-67 pubmed
western blot; mouse; 1:1000; fig 6	Laurent V, Jaubert Miazza L, Desjardins R, Day R, Lindberg I. Biosynthesis of proopiomelanocortin-derived peptides in prohormone convertase 2 and 7B2 null mice. Endocrinology. 2004;145:519-28 pubmed
western blot; mouse; fig 7	Cheng S, Shao J, Charlton Kachigian N, Loewy A, Towler D. MSX2 promotes osteogenesis and suppresses adipogenic differentiation of multipotent mesenchymal progenitors. J Biol Chem. 2003;278:45969-77 pubmed
western blot; human; fig 2b	Kudo Y, Kitajjma S, Sato S, Miyauchi M, Ogawa I, Takata T. Establishment of an oral squamous cell carcinoma cell line with high invasive and p27 degradation activities from a lymph node metastasis. Oral Oncol. 2003;39:515-20 pubmed
immunoprecipitation; common platanna; fig 2	Lessman C, Kim H. Soluble tubulin complexes in oocytes of the common leopard frog, Rana pipiens, contain gamma-tubulin. Mol Reprod Dev. 2001;60:128-36 pubmed
immunocytochemistry; rat; fig 2	Iijima K, Ando K, Takeda S, Satoh Y, Seki T, Itohara S, et al. Neuron-specific phosphorylation of Alzheimer's beta-amyloid precursor protein by cyclin-dependent kinase 5. J Neurochem. 2000;75:1085-91 pubmed
immunocytochemistry; rat; fig 2	Ando K, Oishi M, Takeda S, Iijima K, Isohara T, Nairn A, et al. Role of phosphorylation of Alzheimer's amyloid precursor protein during neuronal differentiation. J Neurosci. 1999;19:4421-7 pubmed
	Velez D, Tsui B, Goshia T, Chute C, Han A, Carter H, et al. 3D collagen architecture induces a conserved migratory and transcriptional response linked to vasculogenic mimicry. Nat Commun. 2017;8:1651 pubmed publisher
	Wallace N, Robinson K, Galloway D. Beta human papillomavirus E6 expression inhibits stabilization of p53 and increases tolerance of genomic instability. J Virol. 2014;88:6112-27 pubmed publisher
	Kaneko Oshikawa C, Nakagawa T, Yamada M, Yoshikawa H, Matsumoto M, Yada M, et al. Mammalian E4 is required for cardiac development and maintenance of the nervous system. Mol Cell Biol. 2005;25:10953-64 pubmed
	Baghdoyan S, Lamartine J, Castel D, Pitaval A, Roupioz Y, Franco N, et al. Id2 reverses cell cycle arrest induced by {gamma}-irradiation in human HaCaT keratinocytes. J Biol Chem. 2005;280:15836-41 pubmed
	Tian F, Byfield S, Parks W, Stuelten C, Nemani D, Zhang Y, et al. Smad-binding defective mutant of transforming growth factor beta type I receptor enhances tumorigenesis but suppresses metastasis of breast cancer cell lines. Cancer Res. 2004;64:4523-30 pubmed
	Akimoto T, Ribar T, Williams R, Yan Z. Skeletal muscle adaptation in response to voluntary running in Ca2+/calmodulin-dependent protein kinase IV-deficient mice. Am J Physiol Cell Physiol. 2004;287:C1311-9 pubmed

product information

Product Type :

Antibody

Product Name :

alpha Tubulin Monoclonal Antibody (TU-01)

Catalog # :

13-8000

Quantity :

100µg

Price :

US 299.00

Clonality :

Monoclonal

Purity :

Ammonium sulfate precipitation, Caprylic acid precipitation, sequential chromatography

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

ELISA: Assay Dependent, Immunocytochemistry: 1:250, Immunofluorescence: 1:250, Immunohistochemistry (Frozen): 1:10-1:100, Immunohistochemistry (Paraffin): 1:10-1:100, Western Blot: 1:250

Species :

Human, Mouse

Clone :

TU-01

Isotype :

IgG1

Storage :

-20°C

Description :

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. [provided by RefSeq, Jul 2008]

Immunogen :

Reacts with peptides that correspond to aa 65-79 in the N-terminus of alpha-tubulin

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay Dependent, Immunocytochemistry: 1:250, Immunofluorescence: 1:250, Immunohistochemistry (Frozen): 1:10-1:100, Immunohistochemistry (Paraffin): 1:10-1:100, Western Blot: 1:250

Aliases :

B-ALPHA-1, LIS3, TUBA3, alpha-tubulin 3, hum-a-tub1, hum-a-tub2, tubulin B-alpha-1, tubulin alpha-1A chain, tubulin alpha-3 chain, tubulin, alpha 3, tubulin, alpha, brain-specific

PubMed References :

10341243, 10936190, 11550276, 12747977, 12925529, 14576186, 14600152, 14672936, 15229108, 15231662, 15340079, 15691830, 15767263, 16314518, 16716194, 17255328, 17484728, 17967421, 18201694, 18344614, 18483213, 18591938, 18632609, 19224336, 19232519, 19695678, 20020938, 20688056, 21029775, 22098537, 24648447

more info or order :

Invitrogen product webpage