Gli3 antibody | GeneTex GTX26050 product information

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product summary

company name :

GeneTex

product type :

antibody

product name :

Gli3 antibody

catalog :

GTX26050

quantity :

50.00μg

price :

259 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

citations: 2

Reference
Hunt R, Bragina O, Drews M, Kasak L, Timmusk S, Valkna A, et al. Generation and characterization of mouse monoclonal antibody 5E1 against human transcription factor GLI3. Hybridoma (Larchmt). 2007;26:231-40 pubmed
Hunt R, Bragina O, Drews M, Kasak L, Timmusk S, Valkna A, et al. Generation and characterization of mouse monoclonal antibody 5E1 against human transcription factor GLI3. Hybridoma (Larchmt). 2007;26:131-9 pubmed

product information

Catalog No :

GTX26050

Product Name :

Gli3 antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Product Type :

Primary antibody

Size :

50.00μg

List Price :

259 USD

list of Pubmed id :

17600494,17725385

Isotype :

IgG

Product Description :

Rabbit polyclonal to Gli3

CrossReactivity :

Hu, Ms, Xl

Background :

Gli3 is belongs to the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. Gli3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli3 is also expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in GLI3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS); an autosomal dominant disorder affecting limb and craniofacial development.

Immunogen :

Synthetic peptide: NEDESPGQTYHRERRNA-C conjugated to KLH, corresponding to amino acids 41-57 of Human GLI3.

Buffer :

0.02M Potassium phosphate, 0.15 M NaCl, pH 7.2, contaning 0.1% sodium azide

Uses :

ELISA, ICC/IF, IHC-Fr, IHC-P, WB

Concentration :

1.21mg/ml

General References :

Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Image Legend_4 :

Western Blot of Rabbit anti-Gli-3 antibody (GTX26050). Lane 1: human brain whole cell lysate. Lane 2: human lung whole cell lysate. Lane 3: human spleen whole cell lysate. Lane 4: mouse brain whole cell lysate. Lane 5: mouse lung whole cell lysate. Load: 20 µg per lane. Primary antibody: Gli-3 antibody at 1:500 for overnight at 4°C. Secondary antibody: infrared rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: Isoforms at ~170-190kDa and ~80kDa. Lane 2 shows what may be truncated Gli-3 (~80kDa). Other band(s): The strong band at ~50 kDa is unknown.