12(S)-HETE ELISA kit | Enzo Life Sciences ADI-900-050 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

ELISA/assay

product name :

12(S)-HETE ELISA kit

catalog :

ADI-900-050

quantity :

96 wells

citations: 14

Reference
Nguyen C, Stadler S, Brenner S, Huttary N, Krieger S, Jäger W, et al. Cancer cell-derived 12(S)-HETE signals via 12-HETE receptor, RHO, ROCK and MLC2 to induce lymph endothelial barrier breaching. Br J Cancer. 2016;115:364-70 pubmed publisher
Yuan Z, Rapoport S. Transient postnatal fluoxetine decreases brain concentrations of 20-HETE and 15-epi-LXA4, arachidonic acid metabolites in adult mice. Prostaglandins Leukot Essent Fatty Acids. 2015;101:9-14 pubmed publisher
Lee A, Cho K, Kim J. MyD88-BLT2-dependent cascade contributes to LPS-induced interleukin-6 production in mouse macrophage. Exp Mol Med. 2015;47:e156 pubmed publisher
Sun L, Xu Y, Han J, Liang H, Wang N, Cheng Y. 12/15-Lipoxygenase metabolites of arachidonic acid activate PPARÎ³: a possible neuroprotective effect in ischemic brain. J Lipid Res. 2015;56:502-14 pubmed publisher
Chu J, Li J, Giannopoulos P, Blass B, Childers W, Abou Gharbia M, et al. Pharmacologic blockade of 12/15-lipoxygenase ameliorates memory deficits, AÎ² and tau neuropathology in the triple-transgenic mice. Mol Psychiatry. 2015;20:1329-38 pubmed publisher
Kiss I, Unger C, Huu C, Atanasov A, Kramer N, Chatruphonprasert W, et al. Lobatin B inhibits NPM/ALK and NF-ÎºB attenuating anaplastic-large-cell-lymphomagenesis and lymphendothelial tumour intravasation. Cancer Lett. 2015;356:994-1006 pubmed publisher
Taylor Fishwick D, Weaver J, Glenn L, Kuhn N, Rai G, Jadhav A, et al. Selective inhibition of 12-lipoxygenase protects islets and beta cells from inflammatory cytokine-mediated beta cell dysfunction. Diabetologia. 2015;58:549-57 pubmed publisher
Tersey S, Maier B, Nishiki Y, Maganti A, Nadler J, Mirmira R. 12-lipoxygenase promotes obesity-induced oxidative stress in pancreatic islets. Mol Cell Biol. 2014;34:3735-45 pubmed publisher
Agbor T, Demma Z, Mrsny R, Castillo A, Boll E, McCormick B. The oxido-reductase enzyme glutathione peroxidase 4 (GPX4) governs Salmonella Typhimurium-induced neutrophil transepithelial migration. Cell Microbiol. 2014;16:1339-53 pubmed publisher
Kretschy N, Teichmann M, Kopf S, Atanasov A, Saiko P, Vonach C, et al. In vitro inhibition of breast cancer spheroid-induced lymphendothelial defects resembling intravasation into the lymphatic vasculature by acetohexamide, isoxsuprine, nifedipin and proadifen. Br J Cancer. 2013;108:570-8 pubmed publisher
Stavniichuk R, Drel V, Shevalye H, Vareniuk I, Stevens M, Nadler J, et al. Role of 12/15-lipoxygenase in nitrosative stress and peripheral prediabetic and diabetic neuropathies. Free Radic Biol Med. 2010;49:1036-45 pubmed publisher
Law I, Xu A, Lam K, Berger T, Mak T, Vanhoutte P, et al. Lipocalin-2 deficiency attenuates insulin resistance associated with aging and obesity. Diabetes. 2010;59:872-82 pubmed publisher
Bernard J, Seweryniak K, Koniski A, Spinelli S, Blumberg N, Francis C, et al. Foxp3 regulates megakaryopoiesis and platelet function. Arterioscler Thromb Vasc Biol. 2009;29:1874-82 pubmed publisher
Mabalirajan U, Aich J, Leishangthem G, Sharma S, Dinda A, Ghosh B. Effects of vitamin E on mitochondrial dysfunction and asthma features in an experimental allergic murine model. J Appl Physiol (1985). 2009;107:1285-92 pubmed publisher

product information

Product ID :

ADI-900-050

Catalog Number :

ADI-900-050

Product Name :

12(S)-HETE ELISA kit

Product Type :

Kit

Size :

96 wells

list of Pubmed id :

26234927, 25838003, 25605873, 25560760, 25444930, 25417214, 25071151, 24617613, 23299527, 20599608

Product Description :

The 12(S)-HETE EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 5 hours. Absorbance is read at 405 nm. Fast, accurate, and reliable results are obtained with ready-to-use liquid color-coded reagents that reduce error.

SPECIES REACTIVITY :

Species independent

Background :

HETEs are byproducts generated by the metabolism of arachidonic acid by lipoxygenases. 12(S)-HETE is the stereospecific hydroxy product from the reduction of 12(S)-hydroperoxy tetraenoic eicosatetraenoic acid [12(S)-HpETE], which itself is a 12-lipoxygenase metabolite of arachidonic acid. 12(S)-HETE is chemotactic and chemokinetic for polymorphonuclear leukocytes and vascular smooth muscle cells. It also acts as a second messenger in angiotensin-II induced aldosterone production. 15-hydroxyeicosatetraenoic acid [15(S)-HETE] is the major hydroxy derivative of arachidonic acid when acted upon by 15-lipoxygenase. It is also the primary monohydroxy acid synthesized by the lipoxygenase activity of Cyclooxygenase-1 upon linoleic acid. 15(S)-HETE acts as a paracrine regulator of smooth muscle and lung neutrophil recruitment, and elevated levels of 15(S)-HETE are associated with asthma, rhinitis, chronic paranasal sinusitis and rheumatoid arthritis.

ALTnames :

12(S)-hydroperoxy tetraenoic eicosatetraenoic acid

Uses :

For the quantitative determination of 12(S)-HETE in culture supernatants and plasma from any species. Cited sample types include serum, renal interstitial fluid and tissue.

Application Summary :

ELISA, Colorimetric detection