product summary
request information :
company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
ELISA/assay
product name :
[pSer9]GSK-3beta ELISA kit
catalog :
ADI-900-123A
quantity :
96 wells
price :
550.00 USD
product information
Catalog Number :
ADI-900-123A
Product Name :
[pSer9]GSK-3beta ELISA kit
Product Type :
Kit
List Price (USD) :
550.00 USD
Product Description :
The [pSer9]GSK-3beta EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours. Save time, money, and precious sample with fully quantitative results and increased sensitivity compared to Western blot analysis.
CrossReactivity :
human, mouse, rat
Background :
Glycogen Synthase Kinase 3beta; (GSK-3beta) is a unique serine/threonine kinase that is inactivated by phosphorylation. In response to insulin binding, PKB/AKT phosphorylates GSK-3beta on serine 9, which prevents GSK-3beta from phosphorylating glycogen synthase. Unphosphorylated glycogen synthase is active and able to synthesize glycogen. GSK-3beta is also unique in that it requires a substrate that has been phosphorylated by a distinct kinase before it can phosphorylate the substrate. This phosphate priming mechanism explains why phosphorylation of serine 9 inactivates GSK-3beta;. The phosphorylated serine binds to the GSK-3beta priming phosphate position and prevents binding of alternative substrates. In addition to insulin signaling, GSK-3beta participates in the Wnt signaling pathway, where it forms a complex with axin, beta-catenin and adenomatous polyposis coli (APC) protein. In the presence of Wnts, GSK-3beta is unable to phosphorylate beta-catenin, which leads to stabilization of beta-catenin. The Wnt pathway inactivates GSK-3beta via the proteins, Dishevelled and FRAT, which disrupt the interaction of GSK-3beta with axin, beta-catenin, and APC. Clinically, there is considerable interest in GSK-3beta inhibitors because they may mimic the effect of insulin or reduce the hyperphosphorylation of Tau that is observed in Alzheimer's Disease.
ALTnames :
Glycogen synthase kinase
Package Size :
96 wells
Sensitivity :
9.0 pg/ml (range 62.5 - 2,000 pg/ml)
Uses :
For the quantitative determination of [pSer9]GSK-3beta in cell lysates of human, mouse, and rat origin.
Application Summary :
ELISA, Colorimetric Detection
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
www.enzolifesciences.com
1-800-942-0430
headquarters: usa
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.