product summary
request information :
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Anti-Active-β-Catenin (Anti-ABC) Antibody, clone 8E7
catalog :
05-665
quantity :
100 μg
clonality :
monoclonal
host :
mouse
reactivity :
zebrafish , human
application :
immunohistochemistry - paraffin section, flow cytometry, immunocytochemistry, immunohistochemistry, western blot
publications citing this reagent :
total: 67
  1. Bisson J, Mills B, Paul Helt J, Zwaka T, Cohen E. Wnt5a and Wnt11 inhibit the canonical Wnt pathway and promote cardiac progenitor development via the Caspase-dependent degradation of AKT. Dev Biol. 2015;398:80-96 pubmed publisher
  2. Easter S, Mitchell E, Baxley S, Desmond R, Frost A, Serra R. Wnt5a suppresses tumor formation and redirects tumor phenotype in MMTV-Wnt1 tumors. PLoS ONE. 2014;9:e113247 pubmed publisher
  3. Kim K, Ossipova O, Sokol S. Neural crest specification by inhibition of the ROCK/Myosin II pathway. Stem Cells. 2015;33:674-85 pubmed publisher
  4. Schlupf J, Steinbeisser H. IGF antagonizes the Wnt/β-Catenin pathway and promotes differentiation of extra-embryonic endoderm. Differentiation. 2014;87:209-19 pubmed publisher
  5. Joseph J, Conroy S, Tomar T, Eggens-Meijer E, Bhat K, Copray S, et al. TGF-β is an inducer of ZEB1-dependent mesenchymal transdifferentiation in glioblastoma that is associated with tumor invasion. Cell Death Dis. 2014;5:e1443 pubmed publisher
  6. Jansen S, Holman R, Hedemann I, Frankes E, Elzinga C, Timens W, et al. Prostaglandin E2 promotes MYCN non-amplified neuroblastoma cell survival via β-catenin stabilization. J Cell Mol Med. 2015;19:210-26 pubmed publisher
  7. Bhushan B, Walesky C, Manley M, Gallagher T, Borude P, Edwards G, et al. Pro-regenerative signaling after acetaminophen-induced acute liver injury in mice identified using a novel incremental dose model. Am J Pathol. 2014;184:3013-25 pubmed publisher
  8. Forsdahl S, Kiselev Y, Hogseth R, Mjelle J, Mikkola I. Pax6 regulates the expression of Dkk3 in murine and human cell lines, and altered responses to Wnt signaling are shown in FlpIn-3T3 cells stably expressing either the Pax6 or the Pax6(5a) isoform. PLoS ONE. 2014;9:e102559 pubmed publisher
  9. Antony L, van der Schoor F, Dalrymple S, Isaacs J. Androgen receptor (AR) suppresses normal human prostate epithelial cell proliferation via AR/β-catenin/TCF-4 complex inhibition of c-MYC transcription. Prostate. 2014;74:1118-31 pubmed publisher
  10. Sun T, Co N, Wong N. PFTK1 interacts with cyclin Y to activate non-canonical Wnt signaling in hepatocellular carcinoma. Biochem Biophys Res Commun. 2014;449:163-8 pubmed publisher
more info or order :
product information
Catalog Number :
05-665
Subcategory :
Epigenetics & Nuclear Function
Product Name :
Anti-Active-β-Catenin (Anti-ABC) Antibody, clone 8E7
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P35222
Host Name :
Mouse
Antigen :
Active-β-Catenin (ABC)
Clone :
8E7
Conjugate :
Purified
Isotype :
IgG1κ
Product Description :
Anti-Active-β-Catenin (Anti-ABC) Antibody, clone 8E7
Cross Reactivity :
Human;Mouse;Rat
Background :
Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as α-catenin and β-catenin. α-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. When β-catenin was sequenced it was found to be a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain which is specialized for protein-protein binding. An increase in β-catenin production has been noted in those people who have Basal Cell Carcinoma and leads to the increase in proliferation of related tumors. When β-catenin is not associated with cadherins and α-catenin, it can interact with other proteins such as Catenin Beta Interacting Protein 1 (ICAT) and adenomatosis polyposis coli (APC). Recent evidence suggests that β-catenin plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.
Immunogen :
Fusion protein corresponding to residues 1-100 of human β-catenin with a c-terminal histidine tag
Specificity :
Recognizes active form of β-catenin, dephosphorylated on Ser37 or Thr41.
Package Size :
100 μg
Uses :
Flow Cytometry;Immunocytochemistry;Immunohistochemistry;Immunohistochemistry (Paraffin);Western Blotting
Storage :
Maintain for 1 year at 2-8°C from date of shipment. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
publications citing this reagent :
  1. Zahoor M, Cha P, Choi K. Indirubin-3'-oxime, an activator of Wnt/β-catenin signaling, enhances osteogenic commitment of ST2 cells and restores bone loss in high-fat diet-induced obese male mice. Bone. 2014;65:60-8 pubmed publisher
  2. Kang W, Tong J, Chan A, Zhao J, Dong Y, Wang S, et al. Yin Yang 1 contributes to gastric carcinogenesis and its nuclear expression correlates with shorter survival in patients with early stage gastric adenocarcinoma. J Transl Med. 2014;12:80 pubmed publisher
  3. Umazume K, Tsukahara R, Liu L, Fernandez de Castro J, McDonald K, Kaplan H, et al. Role of retinal pigment epithelial cell β-catenin signaling in experimental proliferative vitreoretinopathy. Am J Pathol. 2014;184:1419-28 pubmed publisher
  4. Wang H, Sun W, Ma J, Pan Y, Wang L, Zhang W. Polycystin-1 mediates mechanical strain-induced osteoblastic mechanoresponses via potentiation of intracellular calcium and Akt/β-catenin pathway. PLoS ONE. 2014;9:e91730 pubmed publisher
  5. Guezguez A, Paré F, Benoit Y, Basora N, Beaulieu J. Modulation of stemness in a human normal intestinal epithelial crypt cell line by activation of the WNT signaling pathway. Exp Cell Res. 2014;322:355-64 pubmed publisher
  6. Yukata K, Xie C, Li T, Takahata M, Hoak D, Kondabolu S, et al. Aging periosteal progenitor cells have reduced regenerative responsiveness to bone injury and to the anabolic actions of PTH 1-34 treatment. Bone. 2014;62:79-89 pubmed publisher
  7. Xie Q, Chen L, Shan X, Shan X, Tang J, Zhou F, et al. Epigenetic silencing of SFRP1 and SFRP5 by hepatitis B virus X protein enhances hepatoma cell tumorigenicity through Wnt signaling pathway. Int J Cancer. 2014;135:635-46 pubmed publisher
  8. Li C, Zhang S, Lu Y, Zhang Y, Wang E, Cui Z. The roles of Notch3 on the cell proliferation and apoptosis induced by CHIR99021 in NSCLC cell lines: a functional link between Wnt and Notch signaling pathways. PLoS ONE. 2013;8:e84659 pubmed publisher
  9. Svedlund J, Barazeghi E, Stalberg P, Hellman P, Akerstrom G, Björklund P, et al. The histone methyltransferase EZH2, an oncogene common to benign and malignant parathyroid tumors. Endocr Relat Cancer. 2014;21:231-9 pubmed publisher
  10. Fong J, Jacobs R, Moravec D, Uppada S, Botting G, Nlend M, et al. Alternative signaling pathways as potential therapeutic targets for overcoming EGFR and c-Met inhibitor resistance in non-small cell lung cancer. PLoS ONE. 2013;8:e78398 pubmed publisher
  11. Bilir B, Kucuk O, Moreno C. Wnt signaling blockage inhibits cell proliferation and migration, and induces apoptosis in triple-negative breast cancer cells. J Transl Med. 2013;11:280 pubmed publisher
  12. Li L, Ying J, Tong X, Zhong L, Su X, Xiang T, et al. Epigenetic identification of receptor tyrosine kinase-like orphan receptor 2 as a functional tumor suppressor inhibiting β-catenin and AKT signaling but frequently methylated in common carcinomas. Cell Mol Life Sci. 2014;71:2179-92 pubmed publisher
  13. Ghazvini M, Sonneveld P, Kremer A, Franken P, Sacchetti A, Atlasi Y, et al. Cancer stemness in Apc- vs. Apc/KRAS-driven intestinal tumorigenesis. PLoS ONE. 2013;8:e73872 pubmed publisher
  14. Miyazaki T, Miyazaki S, Ashida M, Tanaka T, Tashiro F, Miyazaki J. Functional analysis of Tcl1 using Tcl1-deficient mouse embryonic stem cells. PLoS ONE. 2013;8:e71645 pubmed publisher
  15. Tiwari A, Schneider M, Fiorino A, Haider R, Okoniewski M, Roschitzki B, et al. Early insights into the function of KIAA1199, a markedly overexpressed protein in human colorectal tumors. PLoS ONE. 2013;8:e69473 pubmed publisher
  16. Engert S, Burtscher I, Liao W, Dulev S, Schotta G, Lickert H. Wnt/β-catenin signalling regulates Sox17 expression and is essential for organizer and endoderm formation in the mouse. Development. 2013;140:3128-38 pubmed publisher
  17. Tang Z, Dong Q, Cui Q, Papavassiliou P, Wang E, Wang E. Ataxia-telangiectasia group D complementing gene (ATDC) promotes lung cancer cell proliferation by activating NF-κB pathway. PLoS ONE. 2013;8:e63676 pubmed publisher
  18. Son Y, Pratheeshkumar P, Wang L, Wang X, Fan J, Kim D, et al. Reactive oxygen species mediate Cr(VI)-induced carcinogenesis through PI3K/AKT-dependent activation of GSK-3β/β-catenin signaling. Toxicol Appl Pharmacol. 2013;271:239-48 pubmed publisher
  19. Shao L, Oshima S, Duong B, Advincula R, Barrera J, Malynn B, et al. A20 restricts wnt signaling in intestinal epithelial cells and suppresses colon carcinogenesis. PLoS ONE. 2013;8:e62223 pubmed publisher
  20. Ludwig K, Tse E, Wang J. Colon cancer cells adopt an invasive phenotype without mesenchymal transition in 3-D but not 2-D culture upon combined stimulation with EGF and crypt growth factors. BMC Cancer. 2013;13:221 pubmed publisher
  21. Wang Y, Huang C, Reddy Chintagari N, Bhaskaran M, Weng T, Guo Y, et al. miR-375 regulates rat alveolar epithelial cell trans-differentiation by inhibiting Wnt/β-catenin pathway. Nucleic Acids Res. 2013;41:3833-44 pubmed publisher
  22. Chen J, Chan A, To K, Chen W, Zhang Z, Ren J, et al. SIRT2 overexpression in hepatocellular carcinoma mediates epithelial to mesenchymal transition by protein kinase B/glycogen synthase kinase-3β/β-catenin signaling. Hepatology. 2013;57:2287-98 pubmed publisher
  23. Han J, Soletti R, Sadarangani A, Sridevi P, Ramirez M, Eckmann L, et al. Nuclear expression of β-catenin promotes RB stability and resistance to TNF-induced apoptosis in colon cancer cells. Mol Cancer Res. 2013;11:207-18 pubmed publisher
  24. Thu K, Radulovich N, Becker-Santos D, Pikor L, Pusic A, Lockwood W, et al. SOX15 is a candidate tumor suppressor in pancreatic cancer with a potential role in Wnt/β-catenin signaling. Oncogene. 2014;33:279-88 pubmed publisher
  25. Latasa M, Salis F, Urtasun R, Garcia-Irigoyen O, Elizalde M, Uriarte I, et al. Regulation of amphiregulin gene expression by β-catenin signaling in human hepatocellular carcinoma cells: a novel crosstalk between FGF19 and the EGFR system. PLoS ONE. 2012;7:e52711 pubmed publisher
  26. Li Q, Kannan A, Das A, Demayo F, Hornsby P, Young S, et al. WNT4 acts downstream of BMP2 and functions via β-catenin signaling pathway to regulate human endometrial stromal cell differentiation. Endocrinology. 2013;154:446-57 pubmed publisher
  27. Liu L, Alonso V, Guo L, Tourkova I, Henderson S, Almarza A, et al. Na+/H+ exchanger regulatory factor 1 (NHERF1) directly regulates osteogenesis. J Biol Chem. 2012;287:43312-21 pubmed publisher
  28. Urdinguio R, Fernandez A, Moncada-Pazos A, Huidobro C, Rodriguez R, Ferrero C, et al. Immune-dependent and independent antitumor activity of GM-CSF aberrantly expressed by mouse and human colorectal tumors. Cancer Res. 2013;73:395-405 pubmed publisher
  29. Wei W, Sun H, Ting K, Zhang L, Lee H, Li G, et al. Inhibition of cardiomyocytes differentiation of mouse embryonic stem cells by CD38/cADPR/Ca2+ signaling pathway. J Biol Chem. 2012;287:35599-611 pubmed publisher
  30. Son Y, Wang L, Poyil P, Budhraja A, Hitron J, Zhang Z, et al. Cadmium induces carcinogenesis in BEAS-2B cells through ROS-dependent activation of PI3K/AKT/GSK-3β/β-catenin signaling. Toxicol Appl Pharmacol. 2012;264:153-60 pubmed publisher
  31. Tran T, Shi X, Zaia J, Ai X. Heparan sulfate 6-O-endosulfatases (Sulfs) coordinate the Wnt signaling pathways to regulate myoblast fusion during skeletal muscle regeneration. J Biol Chem. 2012;287:32651-64 pubmed
  32. Ng F, Toh T, Ting E, Koh G, Sandanaraj E, Phong M, et al. Progenitor-like traits contribute to patient survival and prognosis in oligodendroglial tumors. Clin Cancer Res. 2012;18:4122-35 pubmed
  33. Clinkenbeard E, Butler J, Spear B. Pericentral activity of alpha-fetoprotein enhancer 3 and glutamine synthetase upstream enhancer in the adult liver are regulated by β-catenin in mice. Hepatology. 2012;56:1892-901 pubmed publisher
  34. Kohan-Ghadr H, Smith L, Arnold D, Murphy B, Lefebvre R. Aberrant expression of E-cadherin and β-catenin proteins in placenta of bovine embryos derived from somatic cell nuclear transfer. Reprod Fertil Dev. 2012;24:588-98 pubmed publisher
  35. Law P, Ching A, Chan A, Wong Q, Wong C, To K, et al. MiR-145 modulates multiple components of the insulin-like growth factor pathway in hepatocellular carcinoma. Carcinogenesis. 2012;33:1134-41 pubmed publisher
  36. Vinuesa A, Sancho R, García-Limones C, Behrens A, Ten Dijke P, Calzado M, et al. Vanilloid receptor-1 regulates neurogenic inflammation in colon and protects mice from colon cancer. Cancer Res. 2012;72:1705-16 pubmed publisher
  37. Pourreyron C, Reilly L, Proby C, Panteleyev A, Fleming C, McLean K, et al. Wnt5a is strongly expressed at the leading edge in non-melanoma skin cancer, forming active gradients, while canonical Wnt signalling is repressed. PLoS ONE. 2012;7:e31827 pubmed publisher
  38. Jacob F, Ukegjini K, Nixdorf S, Ford C, Olivier J, Caduff R, et al. Loss of secreted frizzled-related protein 4 correlates with an aggressive phenotype and predicts poor outcome in ovarian cancer patients. PLoS ONE. 2012;7:e31885 pubmed publisher
  39. Yoshioka S, King M, Ran S, Okuda H, Maclean J, McAsey M, et al. WNT7A regulates tumor growth and progression in ovarian cancer through the WNT/β-catenin pathway. Mol Cancer Res. 2012;10:469-82 pubmed publisher
  40. Kodithuwakku S, Pang R, Ng E, Cheung A, Horne A, Ho P, et al. Wnt activation downregulates olfactomedin-1 in Fallopian tubal epithelial cells: a microenvironment predisposed to tubal ectopic pregnancy. Lab Invest. 2012;92:256-64 pubmed publisher
  41. Ceballos M, Parody J, Alvarez M, Ingaramo P, Carnovale C, Carrillo M. Interferon-α2b and transforming growth factor-β1 treatments on HCC cell lines: Are Wnt/β-catenin pathway and Smads signaling connected in hepatocellular carcinoma?. Biochem Pharmacol. 2011;82:1682-91 pubmed publisher
  42. Wang D, Dai C, Li Y, Liu Y. Canonical Wnt/β-catenin signaling mediates transforming growth factor-β1-driven podocyte injury and proteinuria. Kidney Int. 2011;80:1159-69 pubmed publisher
  43. Luan L, Ding T, Stinnett A, Reese J, Paria B. Adherens junction proteins in the hamster uterus: their contributions to the success of implantation. Biol Reprod. 2011;85:996-1004 pubmed publisher
  44. Kato H, Gruenwald A, Suh J, Miner J, Barisoni-Thomas L, Taketo M, et al. Wnt/β-catenin pathway in podocytes integrates cell adhesion, differentiation, and survival. J Biol Chem. 2011;286:26003-15 pubmed publisher
  45. Liu J, Wang Z, Tang J, Tang R, Shan X, Zhang W, et al. Hepatitis C virus core protein activates Wnt/β-catenin signaling through multiple regulation of upstream molecules in the SMMC-7721 cell line. Arch Virol. 2011;156:1013-23 pubmed publisher
  46. Mödder U, Oursler M, Khosla S, Monroe D. Wnt10b activates the Wnt, notch, and NFκB pathways in U2OS osteosarcoma cells. J Cell Biochem. 2011;112:1392-402 pubmed publisher
  47. Griffin C, Curtis C, Davis R, Muthukumar V, Magnuson T. The chromatin-remodeling enzyme BRG1 modulates vascular Wnt signaling at two levels. Proc Natl Acad Sci U S A. 2011;108:2282-7 pubmed publisher
  48. Blanchart A, Martín-López E, de Carlos J, Lopez-Mascaraque L. Peripheral contributions to olfactory bulb cell populations (migrations towards the olfactory bulb). Glia. 2011;59:278-92 pubmed publisher
  49. Lussier C, Brial F, Roy S, Langlois M, Verdu E, Rivard N, et al. Loss of hepatocyte-nuclear-factor-1alpha impacts on adult mouse intestinal epithelial cell growth and cell lineages differentiation. PLoS ONE. 2010;5:e12378 pubmed publisher
  50. Winslow B, Burke A. Atypical molecular profile for joint development in the avian costal joint. Dev Dyn. 2010;239:2547-57 pubmed publisher
  51. Behr B, Longaker M, Quarto N. Differential activation of canonical Wnt signaling determines cranial sutures fate: a novel mechanism for sagittal suture craniosynostosis. Dev Biol. 2010;344:922-40 pubmed publisher
  52. Bosco E, Nakai Y, Hennigan R, Ratner N, Zheng Y. NF2-deficient cells depend on the Rac1-canonical Wnt signaling pathway to promote the loss of contact inhibition of proliferation. Oncogene. 2010;29:2540-9 pubmed publisher
  53. Lambertini E, Lisignoli G, Torreggiani E, Manferdini C, Gabusi E, Franceschetti T, et al. Slug gene expression supports human osteoblast maturation. Cell Mol Life Sci. 2009;66:3641-53 pubmed publisher
  54. Warr N, Siggers P, Bogani D, Brixey R, Pastorelli L, Yates L, et al. Sfrp1 and Sfrp2 are required for normal male sexual development in mice. Dev Biol. 2009;326:273-84 pubmed publisher
  55. Jia L, Miao C, Cao Y, Duan E. Effects of Wnt proteins on cell proliferation and apoptosis in HEK293 cells. Cell Biol Int. 2008;32:807-13 pubmed publisher
  56. Welters H, Oknianska A, Erdmann K, Ryffel G, Morgan N. The protein tyrosine phosphatase-BL, modulates pancreatic beta-cell proliferation by interaction with the Wnt signalling pathway. J Endocrinol. 2008;197:543-52 pubmed publisher
  57. human (WB)
    Ray S, Xu F, Wang H, Das S. Cooperative control via lymphoid enhancer factor 1/T cell factor 3 and estrogen receptor-alpha for uterine gene regulation by estrogen. Mol Endocrinol. 2008;22:1125-40 pubmed publisher
more info or order :
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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